Effect of cultivation conditions on glycerophosphate oxidase production by a mutant strain of Aerococcus viridans.

The effect of different concentrations of carbon source (lactose) and inducer (glycerol) on biomass and glycerophosphate oxidase (GPO) production by mutant strain of Aerococcus viridans 1509 was tested. The best combination of lactose and glycerol concentrations for good enzyme productivity was 0.5% lactose and 2.5% glycerol. Further improvement of GPO levels was achieved after scaling-up of the bioprocess and cultivation of the cells in a 3 liter laboratory bioreactor. Using 4.5 x 10(-5) m3 s-1 air flow rate during growth, GPO activity increased 20-times in comparison with cultivation in flasks.