Functional interaction of Yaf2 with the central region of MycN.

MYCN is often amplified in advanced-stage neuroblastomas with the consequence of enhanced MycN protein expression. By employing the yeast two-hybrid system we found that Yaf2 binds to the central region of MycN. Binding was also seen in vitro and in vivo. Ectopically expressed Yaf2, like MycN, is localized in the nuclei of neuroblastoma cells. Endogenous Yaf2 is expressed in all three tested neuroblastoma cell lines, all of which also express MycN. Yaf2 was able to enhance MycN-mediated transactivation from an E-box promoter, deletion of the Yaf2 binding region in MycN abrogates this effect. Thus, the binding of Yaf2 to the central region of MycN is functional in mammalian cells.

DAP-5 is involved in MycN/IFNgamma-induced apoptosis in human neuroblastoma cells.

Death associated protein-5 (DAP-5) is a ubiquitously expressed member of the translation initiation factor eIF4G family that lacks the eIF4E binding site. A dominant negative fragment of DAP-5 protects HeLa cells from IFNgamma-induced cell death. By employing a functional approach we examined the role of DAP-5 in human neuroblastoma cells that are sensitized for IFNgamma-induced apoptosis by tetracycline controlled MYCN expression. DAP-5 fragment transcribed at high levels and translated into a functional miniprotein of 28 kDa protected neuroblastoma cells from IFNgamma-induced apoptosis. Reduced serum levels were toxic to cells constitutively expressing DAP-5 fragment suggesting that DAP-5 protein is essential for both viability and death of human neuroblastoma cells.

Elizabeth Blackwell--the forgotten herbalist?

This article looks at the work of Elizabeth Blackwell (1707-1758), by all accounts the first British female herbalist. To raise funds to free her husband from debtors prison, she produced her hand drawn, engraved and coloured 'Curious Herbal' in 1735. Using the copies of the herbal in the British Library, the article will look at the circumstances surrounding production of the herbal, her influences and sources used, including the people who recommended production of the book to the Society of Apothecaries and some of the current projects to put her name back on the map.

Data security and patient confidentiality: the manager's role.

The maintenance of patient confidentiality is of utmost importance in the doctor patient relationship. With the advent of networks such as the National Health Service Wide Area Network in the UK, the potential to transmit identifiable clinical data will become greater. Links between general practitioners (GPs) and hospitals will allow the rapid transmission of data which if intercepted could be potentially embarrassing to the patient concerned. In 1994 the British Medical Association launched a draft bill on privacy and confidentiality and in association with this bill it is pushing for encryption of all clinical data across electronic networks. The manager's role within an acute hospital, community units and general practice, is to ensure that all employees are aware of the principles of data protection, security of hospital computer systems and that no obvious breaches of security can occur at publicly accessible terminals. Managers must be kept up to date with the latest developments in computer security such as digital signatures and be prepared to instigate these developments where practically possible. Managers must also take responsibility for the monitoring of access to terminals and be prepared to deal severely with staff who breach the code of confidentiality. Each manager must be kept informed of employees status with regard to their 'need to know' clearance level and also to promote confidentiality of patient details throughout the hospital. All of the management team must be prepared to train new staff in the principles of data security as they join the organisation and recognise their accountability if the programme fails. Data security and patient confidentiality is a broad responsibility in any healthcare organisation, with the Chief Executive accountable. In family practice, the partners are responsible and accountable. The British Medical Association believes as a matter of policy, that allowing access to personal health data without the patients consent, except in a legally allowable situation, should be a statutory offence.

Computerized information sources in toxicology and their use in a poisons centre.

The increasing volume of calls received by a poisons centre, such as the National Poisons Unit, Guy's Hospital, London is placing the present information services under escalating pressure. Recent developments in the technologies used to store and manipulate data have resulted in a number of public and private computerized systems being made available. In order to maximize the benefit of the new technologies to a Poisons Centre Information Service it would be desirable to create an integrated system to link the currently unrelated systems. Such an integrated system would be capable of retrieving bibliographic information from bibliographic files similar to MEDLINE and EMBASE and data from source information files such as RTECS and ECDIN, with the user only having to make one enquiry. This paper examines the current position with regard to computerized information sources in toxicology and discusses how an integrated system might improve the efficiency of a poisons centre's information retrieval service and outlines some of the obstacles to the development of such a system.