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1.
Vet J ; 216: 207-9, 2016 Oct.
Article in English | MEDLINE | ID: mdl-27687954

ABSTRACT

Senecavirus A (SV-A) may cause vesicular disease and neonatal mortality in pigs, and was first detected in Brazil in 2015. Samples including tissues and serum from pigs with suspected vesicular diseases were collected from January to August in 2015 from farms in the states of Minas Gerais, Santa Catarina, Goiás and Rio Grande do Sul, Brazil, and tested for the presence of SV-A by reverse transcriptase PCR. All samples were negative for foot and mouth disease virus, as well as 13 other infectious agents associated with vesicular diseases in pigs. SV-A was detected by PCR in 65/265 (24.5%) specimens. A 530 base pair fragment sequenced from the VP1 protein coding region indicated a high genetic distance from SV-A in other countries, but a common origin among the Brazilian isolates.


Subject(s)
Picornaviridae Infections/veterinary , Picornaviridae/physiology , Swine Diseases/epidemiology , Viral Fusion Proteins/genetics , Amino Acid Sequence , Animals , Brazil/epidemiology , Phylogeny , Picornaviridae/genetics , Picornaviridae Infections/epidemiology , Picornaviridae Infections/virology , Reverse Transcriptase Polymerase Chain Reaction/veterinary , Sequence Analysis, RNA/veterinary , Swine , Swine Diseases/virology , Swine Vesicular Disease/virology
2.
J Vet Diagn Invest ; 24(2): 355-8, 2012 Mar.
Article in English | MEDLINE | ID: mdl-22379052

ABSTRACT

Vaccinia virus (VACV) has been associated with zoonotic exanthemic outbreaks affecting bovids and human beings, with significant public health and economic impacts. Rapid and reliable diagnostic methods are needed to detect and epidemiologically monitor antibodies to VACV. The current study describes the development of an immunoperoxidase monolayer assay (IPMA) for detection of total VACV antibodies in bovine serum. The assay was validated by comparison with a plaque reduction neutralization test (PRNT). Kappa index of agreement, diagnostic sensitivity, specificity, and accuracy of the IPMA were -1.008, 100%, 96%, and 98%, respectively, when compared with PRNT on 148 field bovine sera. Repeatability tests on 32 field-positive serum samples revealed that intraclass coefficient correlation was 0.86. In experimentally infected cattle, VACV antibodies were detectable by IPMA 4 days postinfection, which was more than 2 weeks earlier than with the PRNT, indicating that IPMA could be a more sensitive test than the latter. In 4 naturally VACV-diseased cows monitored for 13 months, IPMA could detect VACV antibodies up to 13 months, a longer time than PRNT. The IPMA is simpler to produce and perform when compared with PRNT and is time saving and suitable for large-scale surveys of VACV infection in bovine.


Subject(s)
Antibodies, Viral/blood , Cattle Diseases/virology , Immunoenzyme Techniques/veterinary , Vaccinia virus/isolation & purification , Vaccinia/veterinary , Animals , Brazil , Cattle , Female , Immunoenzyme Techniques/methods , Neutralization Tests/veterinary , Reproducibility of Results , Sensitivity and Specificity , Vaccinia/blood , Vaccinia/virology
3.
Foodborne Pathog Dis ; 6(9): 1141-6, 2009 Nov.
Article in English | MEDLINE | ID: mdl-19888798

ABSTRACT

The vaccinia virus (VACV), which causes exanthemous lesions in dairy cattle and humans, has been associated with several bovine vaccinia outbreaks in Brazil. Currently, no data are available about the safety of milk produced in VACV-affected areas. In this study, 47 milk samples were collected during bovine vaccinia outbreaks and submitted to viral isolation, DNA detection, and nucleotide sequencing of the conserved tk gene. The appearance of characteristic white pocks on the chorioallantoic membranes of chicken eggs, in association with viral cytopathic effects in chicken embryo fibroblasts and phylogenetic data, strongly suggest milk contamination by VACV. This is the first report of VACV detection in and isolation from milk.


Subject(s)
Cattle Diseases/epidemiology , Disease Outbreaks/veterinary , Milk/virology , Vaccinia virus/isolation & purification , Vaccinia/veterinary , Animals , Biological Assay , Brazil/epidemiology , Cattle , Cattle Diseases/transmission , Cattle Diseases/virology , Chick Embryo , DNA, Viral/metabolism , Intercellular Signaling Peptides and Proteins , Peptides/genetics , Peptides/metabolism , Phylogeny , Polymerase Chain Reaction , Thymidine Kinase/genetics , Thymidine Kinase/metabolism , Vaccinia/epidemiology , Vaccinia/transmission , Vaccinia/virology , Vaccinia virus/metabolism , Virion/isolation & purification
4.
Emerg Infect Dis ; 11(12): 1935-8, 2005 Dec.
Article in English | MEDLINE | ID: mdl-16485483

ABSTRACT

Passatempo virus was isolated during a zoonotic outbreak. Biologic features and molecular characterization of hemagglutinin, thymidine kinase, and vaccinia growth factor genes suggested a vaccinia virus infection, which strengthens the idea of the reemergence and circulation of vaccinia virus in Brazil. Molecular polymorphisms indicated that Passatempo virus is a different isolate.


Subject(s)
Phylogeny , Vaccinia virus/classification , Vaccinia virus/isolation & purification , Vaccinia/epidemiology , Vaccinia/virology , Animals , Brazil/epidemiology , Cattle , Cattle Diseases/pathology , Cattle Diseases/virology , Disease Outbreaks , Humans , Occupational Exposure , Vaccinia/veterinary , Vaccinia virus/genetics , Vaccinia virus/pathogenicity , Zoonoses
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