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Mutations and haplotype diversity in 70 Portuguese G6PD-deficient individuals: an overview on the origin and evolution of mutated alleles.

Manco, Licínio; Gonçalves, Paula; Antunes, Patrícia; Maduro, Filomena; Abade, Augusto; Ribeiro, M Letícia.

Haematologica ; 92(12): 1713-4, 2007 Dec.

Article in English | MEDLINE | ID: mdl-18056001

ABSTRACT

G6PD deficiency mutational profile and haplotype diversity using 6 RFLPs (FokI/PvuII/BspHI/PstI/BclI/NlaIII) and a (CTT)(n) microsatellite, were investigated in 70 G6PD-deficient Portuguese individuals. All but one G6PD A-(376G/202A) variants (44/45) have a single haplotype (+/+/-/+/-/+/195). G6PD Betica(376G/968C) alleles (n=10) have a single RFLP haplotype (+/-/-/+/-/+) and 4 different (CTT)(n) repeats. Age estimates based on microsatellite variation suggest that Betica mutation arose 900 generations ago. G6PD SantaMaria(376G/542T) allele was found on haplotype (+/-/-/+/-/+/201) and 10 G6PD variants on RFLP haplotypes (-/-/+/+/-/-), (-/-/+/+/-/+) and (-/-/+/+/+/+).

Subject(s)

Alleles , Evolution, Molecular , Glucosephosphate Dehydrogenase Deficiency/genetics , Glucosephosphate Dehydrogenase/genetics , Microsatellite Repeats/genetics , Point Mutation , Polymorphism, Restriction Fragment Length , Female , Haplotypes , Humans , Male , Portugal

ABSTRACT

Subject(s)

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