ABSTRACT
OBJECTIVE: This study was undertaken to assess whether fine needle aspirates from non-Hodgkin's lymphoma (NHL) could be used for growth fraction analysis with proliferating cell nuclear antigen (PCNA) staining and if there was a relationship between the growth fraction and cytomorphologic classification according to the Kiel classification. STUDY DESIGN: The study group consisted of 40 patients with NHL diagnosed by fine needle aspiration (FNA) cytology. The cytologic classification of the lymphomas was made by two cytopathologists on May-Grünwald-Giemsa-stained slides using the Kiel classification. There were 27 cases of low and 13 of high grade lymphoma. The estimation of the growth fraction was made by PCNA immunoreactivity. The PCNA index was quantitated in smears by counting an average of 1,000 cells, and the count was correlated with the cytomorphologic classification. RESULTS: There was a strong correlation between the PCNA index and lymphoma grading. High grade lymphomas exhibited a mean PCNA positivity of 74.0%, which was significantly higher (P < .001) than that of low grade lymphomas (17.6%). CONCLUSION: Our study showed that PCNA evaluation is suitable for smears obtained by FNA on NHL, correlates with increasing grades of lymphoma according to the Kiel classification and may offer a method of monitoring treatment of lymphoma.
Subject(s)
Lymphoma, Non-Hodgkin/pathology , Proliferating Cell Nuclear Antigen/analysis , Adolescent , Adult , Aged , Biopsy, Needle , Cell Division , Child , Female , Humans , Immunoenzyme Techniques , Lymphoma, Non-Hodgkin/immunology , Male , Middle Aged , PhenotypeSubject(s)
Carcinoma/secondary , Lung Neoplasms/secondary , Thyroid Neoplasms/pathology , Biopsy, Needle , Carcinoma/chemistry , Fatal Outcome , Female , Humans , Immunohistochemistry , Keratins/analysis , Lung Neoplasms/chemistry , Lymphatic Metastasis , Middle Aged , Thyroglobulin/analysis , Thyroid Neoplasms/chemistryABSTRACT
1. Fine needle aspirates from ten patients with high-grade malignant non-Hodgkin's lymphomas were analyzed by cytomorphology and immunocytochemistry. 2. The following morphologic diagnoses were made: lymphoblastic lymphoma (3 cases), Burkitt's lymphoma (3 cases), mixed small and large cell lymphomas with predominance of large cells (2 cases), and centroblastic lymphoma (2 cases). Immunocytochemistry showed a B-cell phenotype in five cases and a T-cell phenotype in four. One case of lymphoblastic lymphoma was negative for the T and B cell markers used. 3. The results of histological and immunohistochemical analyses performed on surgical biopsies from 8 patients confirmed the morphological diagnosis in all cases. Two cases of Burkitt's lymphoma were submitted only to cytological and immunological diagnosis. 4. The high diagnostic accuracy of combined cytomorphology and immunocytochemical assessment of fine needle aspirate samples validates the use of the technique in the diagnostic work-up of high-grade non-Hodgkin's lymphomas.
Subject(s)
Lymphoma, Non-Hodgkin/pathology , Adolescent , Adult , Aged , Biopsy, Needle , Child , Child, Preschool , Cytodiagnosis , Diagnosis, Differential , Female , Humans , Immunohistochemistry , Lymph Nodes/metabolism , Lymph Nodes/pathology , Lymphoma, Non-Hodgkin/diagnosis , Lymphoma, Non-Hodgkin/metabolism , Male , Middle Aged , Staining and Labeling/methodsABSTRACT
Fine needle aspirates from ten patients with high-grade malignant non-Hodgkin's lynmphomas were analyzed by cytomorphology and immunocytochemistry. The following morphologic diagnoses were made: lymphoblastic lymphoma (3 cases), Burkitt's lymphoma (3 cases), mixed small and large cell lymphomas with predominance of large cells (2 cases), and centroblastic lymphoma (2 cases). Immunocytochemistry showed a B-cell phenotype in five cases and a T-cell phenotype in four. One case of lymphoblastic lymphoma was negative for the T and B cell markers used.The results of histological and immunohistochemical analyses performed on surgical biopsies from 8 patients confirmed the morphological diagnosis in all cases. Two cases of Burkitt's lymphoma were submitted only to cytological and immunological diagnosis. The high diagnostic accuracy of combined cytomorphology and immunocytochemical assessmentof fine needle aspirate samples validates the use of the technique in the diagnostic work-up of high-grade non Hodgkin's lymphomas
Subject(s)
Burkitt Lymphoma , Histocytochemistry , Immunologic Tests , Lymphoma, Non-Hodgkin/diagnosis , Lymphoma , SuctionABSTRACT
Trata-se de apresentaçäo de um caso de criptosporidiose intestinal diagnosticada histologicamente em material de autópsia. A paciente era uma criança de 5 meses, internada com diarréia aguda grave, associada a pneumonia por Pneumocystis carinii, sialadenite citomegálica e candidíase oral e cutánea. A presença de infecçöes oportunísticas múltiplas indicaram o diagnóstico de imunodeficiência. Cryptosporidium sp é uma possibilidade etiológica para diarréias agudas em pacientes imunodeprimidos ou imunocompetentes e deve ser procurado em material de autópsia quando näo diagnosticado "in vivo"
