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1.
Mar Environ Res ; 76: 128-37, 2012 May.
Article in English | MEDLINE | ID: mdl-22014762

ABSTRACT

In flowering plants, floral homeotic MADS-box genes, which constitute a large multigene family, play important roles in the specification of floral organs as defined by the ABCDE model. In this study, a MADS-box gene, ZjMADS1, was isolated and characterized from the marine angiosperm Zostera japonica. The predicted length of the ZjMADS1 protein was 246 amino acids (AA), and the AA sequence was most similar to those of the SEPALLATA (SEP) subfamily, corresponding to E-function genes. Southern blot analysis suggested the presence of two SEP3-like genes in the Z. japonica genome. ZjMADS1 mRNA levels were extremely high in the spadices, regardless of the developmental stage, compared to other organs from the reproductive and vegetative shoots. These results suggest that the ZjMADS1 gene may be involved in spadix development in Z. japonica and act as an E-function gene in floral organ development in marine angiosperms.


Subject(s)
Gene Expression Regulation, Plant , MADS Domain Proteins/genetics , MADS Domain Proteins/metabolism , Zosteraceae/genetics , Zosteraceae/metabolism , Amino Acid Sequence , Flowers/growth & development , Gene Dosage , Gene Order , MADS Domain Proteins/chemistry , Molecular Sequence Data , Phylogeny , Plant Proteins/chemistry , Plant Proteins/genetics , Plant Proteins/metabolism , Sequence Alignment , Sequence Homology, Amino Acid , Zosteraceae/classification
2.
Mar Biotechnol (NY) ; 5(2): 194-200, 2003.
Article in English | MEDLINE | ID: mdl-12876656

ABSTRACT

The effect of the water-soluble UV-absorbing substance (UVAS) extracted from the marine red alga Porphyra yezoensis Ueda on UV-dependent thymine photodimer production was investigated. The T<>T pyrimidine-pyrimidone 6-4 dimer and the cyclobutane cis-syn T<>T 5-6 dimer produced by UV irradiation with a xenon lamp were analyzed by reverse-phase high-performance liquid chromatography. Although the dimer production was reduced when the irradiation was filtered through a UVAS solution, it decreased more when thymine was mixed with UVAS. Furthermore, UVAS inhibited the degradation of UV-irradiated thymine. The inhibitory effect of UVAS was significantly greater than that of exogenously added adenine or guanine, which forms complementary base pairs with thymine. These data suggest that in addition to its filtering effect against UV radiation, UVAS also protects thymine by a direct molecule-to-molecule energy transfer process. The protective function of UVAS against UV irradiation is advantageous for this alga under strong UV irradiation.


Subject(s)
Plant Extracts/pharmacology , Plant Proteins/pharmacology , Pyrimidine Dimers/biosynthesis , Rhodophyta/chemistry , Ultraviolet Rays , Absorption , DNA Damage/drug effects , Dimerization , Photochemistry , Plant Extracts/isolation & purification , Plant Extracts/radiation effects , Plant Proteins/isolation & purification , Plant Proteins/radiation effects , Purines/radiation effects , Pyrimidine Dimers/antagonists & inhibitors , Rhodophyta/metabolism
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