ABSTRACT
The inhibitory effect of tazarotene, an acetylenic retinoid, on human dermal fibroblast in vitro was compared to that of all-trans-retinoic acid. The proliferation of fibroblasts was inhibited by both retinoids at the concentration of 1 microM after 5 days of culture. Synthesis of DNA and collagen was inhibited by both retinoids concentration-dependently up to 10 microM, although tazarotene was weaker in the inhibition of collagen synthesis. These results suggest the possible usefulness of tazarotene in the treatment of fibrotic diseases.
Subject(s)
Dermatologic Agents/pharmacology , Fibroblasts/drug effects , Nicotinic Acids/pharmacology , Retinoids/pharmacology , Skin/drug effects , Bromodeoxyuridine/metabolism , Cell Division/drug effects , Cells, Cultured , Collagen/biosynthesis , DNA/biosynthesis , Dermatologic Agents/metabolism , Fibroblasts/cytology , Fibroblasts/metabolism , Fibrosis , Humans , Nicotinic Acids/metabolism , Receptors, Retinoic Acid/classification , Receptors, Retinoic Acid/drug effects , Receptors, Retinoic Acid/metabolism , Retinoids/metabolism , Skin/cytology , Skin/metabolism , Tretinoin/pharmacologyABSTRACT
Two cases of oral leukoplakia were studied immunohistologically and ultrastructurally. By means of avidin-biotin peroxidase complex technique using papillomavirus specific antibody, distinct nuclear staining of epithelial cells of middle and upper layers was observed in both cases. Further evidence for the presence of human papillomavirus was obtained by the electron micrograph, which revealed virus-like particles (30-40 nm in diameter) within the nucleus.
