Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 6 de 6
Filter
Add more filters










Database
Language
Publication year range
1.
J Sci Food Agric ; 99(13): 5680-5686, 2019 Oct.
Article in English | MEDLINE | ID: mdl-31150113

ABSTRACT

BACKGROUND: Poultry meat has high levels of histidine-containing dipeptides (HCD) and consumption of meat rich in HCD may elicit certain health benefits. The aim of this work was to compare the HCD content (anserine and carnosine) in the breast and thigh muscles of two broiler strains differing in growth rate, feeding regime, and age at slaughter. A 3 (production system) × 2 (sex) × 2 (age at slaughter) full factorial arrangement was applied with fast-growing Ross 308 chicks fed ad libitum (ROSS-AL), slow-growing Sasso T451 chicks fed ad libitum (SASSO-AL), and Ross 308 chicks given limited feeding (ROSS-LIM). At the age of 40 and 62 days, eight birds per production system × sex combination were randomly selected for sampling of the breast and thigh muscle. Muscle HCD content was determined by high-performance liquid chromatography (HPLC). RESULTS: Across treatments, levels of anserine were 2.5- and 1.9-fold higher than carnosine in breast and thigh muscle respectively (P < 0.001), and levels of anserine and carnosine were 2.2- and 2.8-fold higher respectively in breast versus thigh muscle (P < 0.001). In breast muscle, SASSO-AL had higher levels of HCD than ROSS-AL and ROSS-LIM (P < 0.001). Considering different market meat types, breast muscle of 62-day-old SASSO-AL birds had more than threefold higher content of HCD compared to thigh muscle of 40-day-old ROSS-AL birds (P < 0.001). CONCLUSION: Large differences in muscle HCD content were found, varying according to type of muscle and broiler. © 2019 Society of Chemical Industry.


Subject(s)
Anserine/analysis , Carnosine/analysis , Chickens/metabolism , Histidine/metabolism , Muscle, Skeletal/chemistry , Animal Feed/analysis , Animals , Anserine/metabolism , Carnosine/metabolism , Chickens/growth & development , Female , Male , Meat/analysis , Muscle, Skeletal/metabolism
2.
Poult Sci ; 98(9): 3811-3817, 2019 Sep 01.
Article in English | MEDLINE | ID: mdl-31065709

ABSTRACT

The grains that form the basis of most commercial chicken diets are rich in cellulose, an unbranched ß-1,4-linked D-glucopyranose polymer, used as a structural molecule in plants. Although it is a predominant polysaccharide in cereal hulls, it is considered an inert non-fermentable fiber. The aim of the current study was to analyze the effect of in-feed supplementation of cellulose on the gut microbiota composition of broilers. Administration of cellulose to chickens, on top of a wheat-based diet, changed the caecal microbiota composition, as determined using pyrosequencing of the 16S rRNA gene. At day 26, a significantly (P < 0.01) higher relative abundance of the Alistipes genus was observed in the caeca of broilers fed the cellulose-supplemented diet, compared to animals fed the control diet. An in vitro batch fermentation assay showed a significant (P < 0.01) growth stimulation of Alistipes finegoldii in the presence of cellulose. In conclusion, in-feed supplementation of cellulose alters the microbiota composition at the level of the phylum Bacteroidetes, specifically the Alistipes genus. This suggests that cellulose is not essentially inert but can alter the gut micro-environment.


Subject(s)
Cecum/drug effects , Cellulose/metabolism , Chickens/physiology , Gastrointestinal Microbiome/drug effects , Animal Feed/analysis , Animals , Cecum/microbiology , Cellulose/administration & dosage , Chickens/microbiology , Diet/veterinary , Dietary Supplements/analysis , Male
4.
Poult Sci ; 97(9): 3138-3148, 2018 Sep 01.
Article in English | MEDLINE | ID: mdl-29796659

ABSTRACT

Viscosity of intestinal contents is known to affect digestion and absorption of nutrients. In most poultry studies, intestinal viscosity has been measured only after complete removal of solid particles by centrifugation. Centrifugation may however remove particles that contribute to viscosity, hence giving rise to an underestimation of viscosity. Two viscosity measurement techniques, one including a centrifugation step (Brookfield) and the other without (Haake), were compared in-vitro to assess whether both techniques result in similar conclusions regarding viscosity in feedstuffs. Two sets of feedstuff preparations were used. The first set was prepared with different combinations of milled feedstuffs in order to have a wide range of viscosity: 100% corn, 25% corn + 75% wheat, 100% wheat, 90% wheat + 10% rye, all mixed with distilled water. In the second set, barley was incubated with different beta-glucanases, and soybean and sunflower meal were incubated with different pectinases, again all mixed with distilled water. Viscosity was assessed using both techniques (Haake and Brookfield equipments) at six different time points. To evaluate the extent of agreement between the two methods, the Lin's concordance correlation coefficient (CCC) was assessed using the percentage of increase in viscosity within each method, based on pairwise feedstuffs comparison (first set), or relative to the feedstuff without enzyme (second set). The rate of the agreement between the two methods was substantial for the first set of feedstuffs (66%) and for the barley diets incubated with beta-glucanases (69%), whereas the CCC score for the soybean meal diets was very poor (2%) and fair for the sunflower meal diets, incubated with pectinases (32%). The lack of agreement for the latter can be explained by the limited variation in viscosity in these low-viscous mixtures. Although the two techniques are considerably different (e.g., with or without preceding particle removal), they seem to render similar conclusions when applied to poultry feedstuffs to identify distinct differences under the tested circumstances.


Subject(s)
Animal Feed/analysis , Animal Husbandry/methods , Gastrointestinal Contents/chemistry , Animal Husbandry/instrumentation , Animals , Centrifugation , Poultry , Viscosity
5.
Physiol Behav ; 104(3): 469-73, 2011 Sep 01.
Article in English | MEDLINE | ID: mdl-21605575

ABSTRACT

The concentration of glucocorticoid metabolites (GCM) in rabbit faeces has been suggested as a non-invasive indicator of stress. In the present study, GCM concentrations were measured in faeces of fattening rabbits kept in groups of eight, at seven different stocking densities (between 5 and 20 animals/m(2)), with or without environmental enrichment (a wooden structure used mainly for gnawing and resting). Transport (30 min) was used as an acute novel stressor to assess the glucocorticoid response to stress under the different housing conditions. GCM concentrations were elevated post-transport (P<0.001). Whilst cage size had no influence on GCM, enrichment reduced GCM concentrations before as well as after transport (P=0.007 in both cases). Effects of cage size and enrichment on growth characteristics were negligible, whilst enrichment decreased cage manipulation and social contact. The results indicate that even short transport durations may be stressful for rabbits, and that enrichment may decrease housing stress. They suggest that measuring baseline GCM concentrations in faeces is a useful tool to evaluate chronic stress in rabbits, whilst measuring the response to a novel stressor did not provide additional insight.


Subject(s)
Behavior, Animal/physiology , Environment , Feces/chemistry , Glucocorticoids/metabolism , Age Factors , Animals , Female , Housing, Animal , Male , Rabbits , Radioimmunoassay
SELECTION OF CITATIONS
SEARCH DETAIL
...