Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 8 de 8
Filter
Add more filters










Database
Language
Publication year range
1.
Vet Rec ; 171(25): 645, 2012.
Article in English | MEDLINE | ID: mdl-23136309

ABSTRACT

The efficacy of chlortetracycline (CTC) in-feed medication to treat pigs with clinical respiratory disease was investigated in a farrow-to-finish pig herd infected with Mycoplasma hyopneumoniae, and with clinical respiratory disease in growing pigs. In total, 533 pigs were included. The animals were vaccinated against M hyopneumoniae and porcine circovirus type 2 at weaning. At onset of clinical respiratory disease, they were randomly allocated to one of the following treatment groups: chlortetracycline 1 (CTC1) (two consecutive weeks, 500 ppm), chlortetracycline 2 (CTC2) (two non-consecutive weeks, with a non-medicated week interval in between, 500 ppm) or tylosin (T) (three consecutive weeks, 100 ppm). Performance (daily weight gain, feed conversion ratio), pneumonia lesions at slaughter and clinical parameters (respiratory disease score) were assessed. Only numeric differences in favour of the CTC2 group were obtained for the performance and the clinical parameters. The prevalence of pneumonia lesions was 20.5, 13.1 and 23.0 per cent (P<0.05) for the CTC1, CTC2 and T groups, respectively. The study demonstrated that CTC, when administered at onset of clinical respiratory disease via the feed at a dose of 500 ppm during two alternative weeks, was able to decrease the prevalence of pneumonia lesions, and numerically reduce performance losses and clinical signs.


Subject(s)
Anti-Bacterial Agents/administration & dosage , Chlortetracycline/administration & dosage , Mycoplasma hyopneumoniae , Pneumonia of Swine, Mycoplasmal/drug therapy , Swine Diseases/drug therapy , Animal Feed , Animals , Anti-Bacterial Agents/therapeutic use , Chlortetracycline/therapeutic use , Disease Outbreaks/prevention & control , Disease Outbreaks/veterinary , Dose-Response Relationship, Drug , Pneumonia of Swine, Mycoplasmal/prevention & control , Random Allocation , Swine , Swine Diseases/prevention & control , Treatment Outcome , Tylosin/administration & dosage , Tylosin/therapeutic use , Weight Gain
2.
Vet J ; 177(1): 104-9, 2008 Jul.
Article in English | MEDLINE | ID: mdl-17512765

ABSTRACT

Although insufficient milk production in lactating sows may cause tremendous economic losses, reliable methods for estimating milk production in sows under field conditions are not available. This study aimed to investigate whether urine parameters could be used to predict milk production in sows. The milk production of 18 sows was determined during early and mid-lactation. Morning (a.m.) and afternoon (p.m.) urinary levels of potassium (K), sodium (Na), calcium (Ca), magnesium (Mg), lactose and creatinine were analysed. The absolute concentrations, the ratios relative to creatinine, and the fractional excretions of all elements in urine were not significantly associated with milk production. The p.m./a.m. ratios of K, Na and Ca concentrations in urine (K(R), Na(R), and Ca(R)) were significant predictors for milk production, but only during mid-lactation. The total variation in milk production (r(2) value) explained by K(R), Na(R), Ca(R) amounted to 72%, 55%, 42%, respectively. Analysis of minerals and especially K in the a.m. and p.m. urine of sows during mid-lactation provided an acceptable indication of milk production. Further research is necessary to investigate whether the present results can be used to estimate milk production in hypogalactic sows under field conditions.


Subject(s)
Lactation/urine , Milk/metabolism , Swine , Urine/chemistry , Animals , Biomarkers/urine , Calcium/urine , Circadian Rhythm , Female , Lactation/physiology , Pilot Projects , Potassium/urine , Sodium/urine , Urinalysis/methods , Urinalysis/veterinary
3.
Theriogenology ; 68(6): 896-901, 2007 Oct 01.
Article in English | MEDLINE | ID: mdl-17719623

ABSTRACT

The aim of the present study was to assess the effects of porcine circovirus type 2 (PCV2) on porcine embryos and their receptor sows during the first 21 days of pregnancy. Hatched blastocysts exposed to 10(5.0) TCID50 PCV2 per ml (strain 1121, fifth passage PK15) and negative control embryos were transferred to PCV2-immune receptor sows at the 7th day of the cycle. Two weeks after transfer (D21), the receptor sows were euthanized and embryos were recovered. They were assessed macroscopically for viability and examined for viral antigen-positive cells by immunoperoxidase staining. The embryonic survival rate of the PCV2-exposed embryos (6.4%, 7 viable embryos out of 110 transferred) was significantly lower than the survival rate of the negative control embryos (65.4%, 34 viable embryos out of 52 transferred). All of the non-viable PCV2-exposed embryos (n=9) displayed immunohistochemical positive signals for PCV2-antigen in degenerated tissues. In the PCV2-exposed embryos that were categorized as viable at D21, small clusters (n=4) or no PCV2-positive cells (n=3) were detected. The pregnancy results of the receptor sows that received PCV2-exposed embryos (1/5) were considerably different from the negative control receptors (2/2), with 3 out of 5 sows displaying a regular return to oestrus. In conclusion, it can be stated that PCV2 can replicate in embryos and might lead to embryonic death. In a small proportion of embryos, PCV2 exposure does not have a detrimental effect on embryo development before D21.


Subject(s)
Circoviridae Infections/embryology , Circoviridae Infections/veterinary , Circovirus/physiology , Gestational Age , Pregnancy Complications, Infectious/veterinary , Swine/embryology , Swine/virology , Animals , Circoviridae Infections/diagnosis , Circoviridae Infections/immunology , Circovirus/immunology , Embryo Transfer , Embryonic Development/physiology , Female , Male , Pregnancy , Serologic Tests , Titrimetry
4.
Biol Reprod ; 76(3): 415-23, 2007 Mar.
Article in English | MEDLINE | ID: mdl-17123941

ABSTRACT

In the present study, the in vitro interaction of embryos with pseudorabies virus (PRV) and porcine reproductive and respiratory syndrome virus (PRRSV) was investigated by viral antigen detection and by evaluating the expression of virus receptors, namely, poliovirus receptor-related 1 (PVRL1; formerly known as nectin 1) for PRV and sialoadhesin for PRRSV. Embryonic cells of zona pellucida intact embryos incubated with PRV remained negative for viral antigens. Also, no antigen-positive cells could be detected after PRV incubation of protease-treated embryos, since the protease disrupted the expression of PRVL1. However, starting from the five-cell-stage onwards, viral antigen-positive cells were detected after subzonal microinjection of PRV. At this stage, the first foci of PVRL1, also a known cell adhesion molecule, were expressed. At the expanded blastocyst stage, a lining pattern of PVRL1 in the apicolateral border of trophectoderm cells was present, whereas the expression in the inner cell mass was low. Furthermore, PVRL1-specific monoclonal antibody CK41 significantly blocked PRV infection of trophectoderm cells of hatched blastocysts, while the infection of the inner cell mass was only partly inhibited. Viral antigen-positive cells were never detected after PRRSV exposure of preimplantation embryos up to the hatched blastocyst stage. Also, expression of sialoadhesin in these embryonic stages was not detected. We conclude that the use of protease to investigate the virus embryo interaction can lead to misinterpretation of results. Results also show that blastomeres of five-cell embryos up to the hatched blastocysts can become infected with PRV, but there is no risk of a PRRSV infection.


Subject(s)
Blastocyst/physiology , Blastocyst/virology , Herpesvirus 1, Suid/pathogenicity , Porcine respiratory and reproductive syndrome virus/pathogenicity , Receptors, Virus/metabolism , Animals , Antibodies, Monoclonal/pharmacology , Blastocyst/drug effects , Blastomeres/virology , Cell Adhesion Molecules/metabolism , Disease Susceptibility , Female , Male , Membrane Glycoproteins/metabolism , Nectins , Porcine Reproductive and Respiratory Syndrome/embryology , Porcine Reproductive and Respiratory Syndrome/virology , Pregnancy , Pseudorabies/embryology , Pseudorabies/virology , Receptors, Immunologic/metabolism , Sialic Acid Binding Ig-like Lectin 1 , Swine , Swine Diseases/virology
5.
Article in English | MEDLINE | ID: mdl-15525358

ABSTRACT

The present study investigated risk factors for mortality in grow-finishing pigs of 137 pig herds belonging to one integration company during a period of 2.5 years. Mortality data, expressed as the number of dead pigs divided by the number of pigs placed in the fattening unit were investigated retrospectively. The following potential risk factors were evaluated: type of pig herd, season and year of placement in the fattening unit, pig density in the municipality, management practices (density of the pigs in the barn, origin of the pigs), housing conditions and feeding practices. The overall average mortality percentage was 4.70%. Three variables in a multivariable regression model were significantly associated with mortality: season of placement in the fattening unit, origin of the piglets and duration of the fattening period. Pigs placed in October, November and December, were at higher risk than pigs placed in other months. Herds that purchased pigs from a merchant, used pigs from other herds with an excess of piglets for filling their fattening units or herds that purchased pigs from more than five origin herds also suffered higher mortality. The mortality also increased in case of longer duration of the fattening period. This study documented for the first time that in addition to seasonal effects and a longer duration of the fattening period, purchasing feeder pigs from one or a limited number of herds is of crucial importance to achieve low mortality in the grow-finishing pigs.


Subject(s)
Meat , Swine Diseases/mortality , Animal Husbandry , Animals , Belgium/epidemiology , Housing, Animal , Retrospective Studies , Risk Factors , Seasons , Swine
6.
Vet J ; 168(3): 317-22, 2004 Nov.
Article in English | MEDLINE | ID: mdl-15501150

ABSTRACT

Urolithiasis in sows and neonatal pigs is well-known, but information on its occurrence and impact in finishing pigs is sparse. This study reports three outbreaks of urolithiasis in finishing pigs. In one herd, no symptoms were observed, whereas in the other herds the presence of calculi caused obstruction of the urinary tract resulting in death. Using infra-red spectroscopy, the predominant mineral-type found in the uroliths was calcium carbonate (calcite). Only small amounts of calcium oxalate (< 1%) could be detected. A high urinary pH, small abnormalities in the mineral composition of the feed and insufficient drinking water were the most important risk factors identified. To prevent urolithiasis, it is important to ensure adequate water intake, to provide a balanced mineral diet, and to avoid urinary tract infections.


Subject(s)
Swine Diseases/epidemiology , Urinary Calculi/veterinary , Animals , Belgium/epidemiology , Calcium Carbonate/analysis , Calcium Carbonate/urine , Disease Outbreaks/veterinary , Fatal Outcome , Female , Male , Swine , Swine Diseases/pathology , Urinary Calculi/epidemiology , Urinary Calculi/pathology
7.
Theriogenology ; 61(1): 91-101, 2004 Jan 01.
Article in English | MEDLINE | ID: mdl-14643864

ABSTRACT

The aim of the present study was to determine if porcine circovirus type 2 (PCV2) is able to infect embryonic cells of in vivo produced porcine embryos with and without zona pellucida (ZP). ZP-intact and ZP-free morulae (6-day post-insemination) and early blastocysts (7-day post-insemination), and hatched blastocysts (8-day post-insemination) were exposed to 10(5.0) TCID50 PCV2 per ml (strain 1121, fifth passage PK15). At 48 h post-incubation, the percentage of infected embryos and the percentage of viral antigen-positive cells per embryo were determined by indirect immunofluorescence (IF). Significantly different percentages of infected embryos were detected: 15% for ZP-free morulae, 50% for ZP-free early blastocysts and 100% for hatched blastocysts. The percentage of cells that expressed viral antigens was similar for the three stages of development. PCV2 exposure did not affect the in vitro development of the embryos during the 48 h study period. All ZP-intact embryos remained negative for viral antigens. In an additional experiment the diameter of the channels in the porcine ZP was determined. After incubation of early blastocysts with fluorescent microspheres of three different sizes, beads with a diameter of 20 nm and beads with a diameter of 26 nm crossed the zona whereas beads with a diameter of 200 nm did not. In conclusion, it can be stated that PCV2 is able to replicate in in vivo produced ZP-free morulae and blastocysts and that the susceptibility increases during development. The ZP forms a barrier to PCV2 infection, but based on the size of the channels in the ZP the possibility that PCV2 particles cross the ZP cannot be excluded.


Subject(s)
Circoviridae Infections/veterinary , Circovirus , Embryo, Mammalian/virology , Swine Diseases/virology , Swine/embryology , Animals , Antigens, Viral/analysis , Blastocyst/virology , Cell Membrane Permeability , Circoviridae Infections/virology , Circovirus/immunology , Circovirus/isolation & purification , Embryonic and Fetal Development , Fluorescent Antibody Technique, Indirect , Fluorescent Dyes , Microspheres , Morula/virology , Time Factors , Zona Pellucida/physiology , Zona Pellucida/ultrastructure , Zona Pellucida/virology
8.
Theriogenology ; 60(8): 1435-43, 2003 Nov.
Article in English | MEDLINE | ID: mdl-14519465

ABSTRACT

Addition of prostaglandin F2alpha (PGF2alpha) to extended boar semen has been shown to slightly increase reproductive parameters in sows such as the conception rate and the total number of piglets born alive. The mechanisms by which PGF2alpha affect these parameters have not yet been elucidated, but it is possible that the sperm transport after insemination is increased. This study investigated whether the sperm motility from 20 Piétrain boars improved when PGF2alpha (Dinolytic; 5 mg PGF2alpha/ml) was added to diluted semen. Different amounts of PGF2alpha (0, 0.5, 1 and 2 ml/100 ml) were tested and the motility was evaluated immediately after addition of PGF2alpha, after 30 min, 2 h, and 24 h. Two computer-assisted semen analysis (CASA) systems, namely the Sperm Quality Analyzer (SQA-IIC) and the Hamilton Thorne (HTR Ceros 12.1) were used to assess the motility parameters. With the SQA-IIC, sperm motility index values of the treated groups were only slightly higher (P>0.05) compared to the negative control group. The different motility parameters measured with the HTR Ceros 12.1 were similar between the treatment groups, except for beat cross frequency, which was higher in the control group (1.5-5%; P<0.001). This study documented that the addition of 2.5, 5 or 10 mg PGF2alpha to 100 ml diluted boar sperm does not increase any sperm motility parameter. Further research is necessary to elucidate mechanisms by which PGF2alpha in diluted semen may improve the reproductive performance in swine farms.


Subject(s)
Dinoprost/pharmacology , Sperm Motility/drug effects , Swine , Animals , Computers , Kinetics , Male , Semen/cytology
SELECTION OF CITATIONS
SEARCH DETAIL
...