Plasma membrane depolarization precedes photosynthesis damage and long-term leaf bleaching in (E)-chalcone-treated Arabidopsis shoots.

The plant phenolic compound (E)-chalcone has been previously found to induce noticeable seedling size reduction and progressive de-greening (bleaching) in shoots of Arabidopsis thaliana seedlings. In this work, we demonstrate that this progressive de-greening occurring on Arabidopsis shoots after (E)-chalcone treatment, is directly linked to early plasma membrane depolarization and dramatic effects on chloroplasts structure and function. Later effects in chalcone-treated seedlings included ROS accumulation, pigment degradation, reduced photosynthetic activity, bleaching, and eventually cell death. De-greening and pigment degradation induced by (E)-chalcone were partially reversed when NaCl was added together with chalcone, which could be related to restoration of altered pH gradients. All these results suggest that rapid alteration of plasma membrane potential after chalcone treatment is a major component of the mode of action of (E)-chalcone on Arabidopsis metabolism.

[Colitis caused by cytomegalovirus in acquired immunodeficiency syndrome. Report of 2 cases of autopsy]. / Colitis por citomegalovirus en el síndrome de inmunodeficiencia adquirida. Presentación de 2 casos de autopsia.

Two autopsy cases of CMV colitis in AIDS are reported. Intestinal manifestations lasted 2 and 5 months in each case and included: diarrhea and abdominal colic pain. The causes of death were: disseminated CMV infection in case 1 and miliary tuberculosis in case 2. In case 1 small ulcers were apparent in the ileum and the cecum. Microscopic foci of necrosis were also observed. Case showed: sigmoid dilatation with mucosal hemorrhages, small acute ulcers and submucosal fibrosis in the transverse portion of the colon. In both cases cytomegalic inclusion bodies within the endothelial cells of the capillaries at the bottom and the margin of the ulcers were observed. We compare our findings with those in other recently published cases.

Colitis por citomegalovirus en el síndrome de inmunodeficiencia adquirida. Presentación de 2 casos de autopsia / [Colitis caused by cytomegalovirus in acquired immunodeficiency syndrome. Report of 2 cases of autopsy]

Two autopsy cases of CMV colitis in AIDS are reported. Intestinal manifestations lasted 2 and 5 months in each case and included: diarrhea and abdominal colic pain. The causes of death were: disseminated CMV infection in case 1 and miliary tuberculosis in case 2. In case 1 small ulcers were apparent in the ileum and the cecum. Microscopic foci of necrosis were also observed. Case showed: sigmoid dilatation with mucosal hemorrhages, small acute ulcers and submucosal fibrosis in the transverse portion of the colon. In both cases cytomegalic inclusion bodies within the endothelial cells of the capillaries at the bottom and the margin of the ulcers were observed. We compare our findings with those in other recently published cases.

Colitis por citomegalovirus en el síndrome de inmunodeficiencia adquirida. Presentación de 2 casos de autopsia. / [Colitis caused by cytomegalovirus in acquired immunodeficiency syndrome. Report of 2 cases of autopsy]

Two autopsy cases of CMV colitis in AIDS are reported. Intestinal manifestations lasted 2 and 5 months in each case and included: diarrhea and abdominal colic pain. The causes of death were: disseminated CMV infection in case 1 and miliary tuberculosis in case 2. In case 1 small ulcers were apparent in the ileum and the cecum. Microscopic foci of necrosis were also observed. Case showed: sigmoid dilatation with mucosal hemorrhages, small acute ulcers and submucosal fibrosis in the transverse portion of the colon. In both cases cytomegalic inclusion bodies within the endothelial cells of the capillaries at the bottom and the margin of the ulcers were observed. We compare our findings with those in other recently published cases.