[Antagonistic activity of bacteria from Bacillus genus against dermatophyte fungi].

Antagonistic properties of the strain Bacillus subtilis IB-54 with respect to dermatophyte fungi Trichophyton rubrum, T. mentagrophytes var. gypseum, Microsporum canis was studied. The studied strains of bacilli effectively inhibited growth and development of dermatophytes when were cultivated on the media containing different carbon sources. Experiments on laboratory animals showed that B. subtilis IB-54 displayed no virulence, toxicity, and toxigenicity and can be considered as perspective object for development of antimycotic drugs.

[Detection of cytomegalovirus antibody activity in intravenous preparations of immunoglobulins with parallel-line method].

The study demonstrated a possibility to detect specific anti-CMV antibody activity in intravenous preparations of immunoglobulins by qualitative immunoenzyme analysis using parallel-line method. Independently of production technology, specificity, and the activity level, the preparations had a dose-response curve that was parallel to that of a standard sample with five-point linearity at double-step dilution. This makes it possible to use the test system DS-ELISA-ANTI-CMV-G to detect anti-CMV antibody activity in ready-for-use preparations.

[Substantiation of the composition, technology and standardization of the original medicinal forms of interferon and bactisporin].

New medicinal forms of immunobiological preparations--human lekocytic interferon and bactisporin--have been developed for local application in dentistry, surgery, gynecology. As the basis for these preparations, the pharmacopeial collagen solution has been used. The results obtained in this investigation indicate that the preparations are safe and possess wound-healing properties.

[Early diagnostics of hemorrhagic fever with renal syndrome on the basis of the use of Pumala virus recombinant nucleocapsid protein]. / Ranniaia diagnostika gemorragicheskoi likhoradki s pochechnym sindromom na osnove ispol'zovaniia rekombinantnogo nukleokapsidnogo belka virusa Pumala.

Pumala virus recombinant nucleocapsid protein was used for the early diagnosis of haemorrhagic fever with renal syndrome. Specific IgM in the sera of patients could be determined by the IEA technique as early as on days 2-3 from the onset of the disease. The diagnostic effectiveness of the test-system was 95% and its specificity was 98%.

[Humoral immunity of persons inoculated with cultured antirabies vaccines according to abridged schedules]. / Izuchenie gumoral'nogo immuniteta u liudei, privitykh kul'tural'nymi antirabicheskimi vaktsinami po ukorochennym skhemam.

The production of virus-neutralizing antibodies in high titers has been found to occur in persons immunized with tissue-culture rabies vaccines, concentrated and non concentrated, intramuscularly in a dose of 1 ml in accordance with reduced immunization schedules.

[Specific activity of an UV-inactivated antirabies vaccine made from brain tissue administered in a shortened schedule]. / Spetsificheskaia aktivnost' inaktivirovannoi UF-luchami antirabicheskoi vaktsiny iz tkani mozga pri sokrashchennoi skheme vvedeniia.

The results obtained in the study of the specific potency of rabies vaccine prepared from sheep brain tissue and inactivated by UV irradiation indicate that, even in the presence of the lowest immunogenicity index (0.5), 5-6 injections of the vaccine, made not daily, but at interval of 3 and 7 days, induced the production of antibodies in the titers not lower than those resulting from 14-20 daily injections of the same vaccine or Fermi vaccine. The preparation inactivated by UV irradiation should be introduced for therapy according to the shortened immunization schedule with intervals, taking into account the immunogenicity index.