Changes in emulsifying properties of caseinate-Soy soluble polysaccharides conjugates by ultrasonication.

This research aimed to assess the impact of ultrasonication on the emulsifying ability of a conjugate system composed of sodium caseinate and soluble soy polysaccharides. The study analyzed the characteristics of the particles and evaluated the emulsions produced using nanoconjugates. The results showed that ultrasonication improved the contact angle (63.7°) and decreased particle size (75 nm), resulting in more effective emulsifying efficiency. At a 2 % concentration of the nanoconjugates, stable emulsions with a 50 % oil content were successfully formed through complete coverage of the droplets' surface, and no oil release was observed. Moreover, the emulsions' creaming index remained below 25 % even after 60 days of storage. The stability of the nanoconjugate-based emulsions depended on the concentration of nanoconjugates, with an optimal concentration of 4 %. These findings suggest that the nanoconjugates have great potential as a natural stabilizer for emulsion-based products.

Development of a whey-based beverage with enhanced levels of conjugated linoleic acid (CLA) as facilitated by endogenous walnut lipase.

In this study, optimization of fermentation conditions, and applying endogenous walnut lipase were investigated for the manufacture of a fermented, whey-based beverage containing conjugated linoleic acid (CLA). Among different commercial starter and probiotic cultures, the culture containing Lactobacillus delbrueckii subsp. bulgaricus and Streptococcus thermophilus showed high potency for CLA synthesis. The fermentation time and the type of walnut oil (lipolyzed or non-lipolyzed) had significant effects on CLA production, as the highest CLA content (36 mg/g fat) was synthesized in the sample containing 1 % lipolyzed walnut oil fermented at 42 °C for 24 h. Moreover, fermentation time had the highest contribution on viable cell counts, proteolysis, DPPH scavenging activity, and final pH. A significant and positive correlation between cell counts and CLA content was also observed (r = +0.823, p < 0.05). This study establishes a cost effective approach for converting cheese whey to a value added beverage enriched with CLA.

Fabrication of active whey Protein isolate/oleic acid emulsion based film as a promising bio-material for cheese packaging.

Emulsion is a colloidal dispersion for delivering natural antimicrobial, antioxidant, and bioactive compounds to improve the product's quality. The present study aimed to develop the active emulsion film based on whey protein isolate (WPI) by adding oleic acid (OA) (0, 10, and 20% w/w) and green tea extract (GTE) (0 and 0.5% w/v) for cheese packaging. Results showed that the opacity, flexibility, and water barrier properties of WPI hydrogel-based film were significantly increased by adding 10% OA. Active release from emulsion-based films was governed by pH and the nature of food model systems. The minimum release occurred in the acidic food model system. After 5 h exposure in the acid food model system, the obtained release from the active film containing OA 0, 10, and 20% was 52.39, 48.97, and 57.24% of incorporated GTE, respectively. The log reduction value (LRV) of active emulsion film against bacteria was significantly affected by the food model system. Moreover, packed lactic coagulated cheese delivered more phenolic compound of GTE than processed spread cheese. Korsmeyer-Peppas model and Weibull with lag were suggested as appropriate models to forecast the release kinetic of GTE.

Application of multi-criteria decision-making for optimizing the formulation of functional cookies containing different types of resistant starches: A physicochemical, organoleptic, in-vitro and in-vivo study.

This research aimed to develop a healthy cookie formulation containing different types of resistant starch, through the application of TOPSIS approach, as a potent feature of MCDM methodologies. Physicochemical investigations reveled that a harder, denser and less sticky dough was produced by the addition of both types of RS. The baking of these doughs resulted in the production of crumblier cookies of less spread ratio, lower porous crumb and whiter surface/crumb. Moreover, in-vitro digestibility of the cookies demonstrated that the baking process can adversely reduce the resistance of RS4 to the enzymolysis reactions. This phenomenon was further corroborated by in-vivo studies where the RS4 enriched cookies were less capable in reducing the postprandial blood glucose. TOPSIS, through successful solving of the multiple criteria decision 9 (alternatives) × 15 (evaluated attributes) matrix suggested that the cookie containing 15% RS is the best alternative in all aspects, possessing acceptable physicochemical/organoleptic attributes, and in-vivo/in-vitro dietary fiber.

Improving the emulsifying properties of sodium caseinate through conjugation with soybean soluble polysaccharides.

This study was conducted to investigate the impact on the techno-functionality over sodium caseinate (NaCS) when are conjugated with soluble soybean polysaccharides (SSPS). NaCS/SSPS conjugates were prepared through the Maillard reaction using dry heating. The formation of covalent binding between NaCS and SSPS and structural changes of NaCS during glycation were confirmed via SDS-PAGE and ATR-FTIR. A positive correlation was observed between the increase in the browning index of samples and Amadori compounds formation over time, based on the colorimetric results. Emulsions stabilization using conjugates with a higher NaCS/SSPS ratio led to a decreasing trend in the droplets' size and creaming index. Meanwhile, higher viscosity and shear-thinning behavior were observed in conjugate-based emulsions. Finally, conjugates prepared with the NaCS/SSPS ratio of 9/1 at an incubation time of 24 h presented a higher pH and thermal stability and better performance in emulsion stabilization in comparison with each of the biopolymers alone.

Development and characterization of a novel multifunctional film based on wheat filter flour incorporated with carvacrol: Antibacterial, antifungal, and insecticidal potentials.

Wheat filter flour is a by-product derived from the modern wheat milling process. In this study, the influence of plasticizer type (glycerol (G) and sorbitol (S)) and content (25, 35, and 45 g/100 g polymer) on the wheat filter flour-based film was evaluated. Regardless of plasticizer type, increasing the plasticizer content enhanced moisture content, water solubility, and water vapor permeability of film samples. The S-plasticized films presented the greatest tensile strength and the lowest EAB%. The scanning electron microscope observations confirmed the uniform structure of G-plasticized film. Moreover, antimicrobial and physico-mechanical properties of G-plasticized (25%) film were evaluated at the presence of carvacrol (5 and 10 g/100 g polymer). The considerable improvement was achieved in water affinity (14.2%) and flexibility (8.6%) by incorporating 10% carvacrol in G-plasticized films. The greatest inhibitory properties of active wheat filter flour films were observed against Aspergillus niger. By increasing the carvacrol concentration in film-forming solution, the inhibitory activity against Listeria monocytogenes and Escherichia coli in the liquid food model system was increased by 90.3% and 66.95%, respectively. Moreover, the active wheat filter flour-based film released a considerable insecticidal activity against Sitophilus granarius and Tribolium confusum. This work offers a novel utilization of wheat filter flour as an inexpensive blend polymer to manufacture multifunctional active film, which provides a promising approach for pest management besides enhancing the safety of products.

Estimation of oxidative indices in the raw and roasted hazelnuts by accelerated shelf-life testing.

To estimate the oxidative stability of the raw and roasted hazelnuts, accelerated shelf-life testing (ASLT) was used at elevated temperatures (55, 65 and 75 °C) at water activity (aw) of 0.43. Chemical parameters, including peroxide value (PV), para-anisidine value, and total oxidation value were measured to estimate the oxidative stability of the samples using Arrhenius model. In addition, the samples were maintained for 8 months in a real condition at 20-30 °C (long-term shelf-life testing) for validating the results obtained from short-term ASLT. The maximum activation energy (Ea, 78.76 kJ/mol °K) and Q10 (1.871) was obtained for PV in raw hazelnuts, while the minimum Ea (53.36 kJ/mol °K) and Q10 (1.552) were recorded for PV in roasted hazelnuts, indicating the negative effect of roasting process on the oxidative stability of the samples. In order to validate the estimations, the values predicted by short-term ASLT for each oxidation index were plotted versus their corresponding values in actual storage. The results showed good correlation coefficients (R2 = 0.91-0.98), confirming the fitness of the Arrhenius model to predict the oxidative indices of the samples during normal storage.

Evaluation of release mechanism of catechin from chitosan-polyvinyl alcohol film by exposure to gamma irradiation.

This study was conducted to investigate the application of gamma irradiation as a means to control the release rate of catechin from polymer matrix. First, chitosan and polyvinyl alcohol (1:3) were mixed and later chitosan nanoparticles containing catechin were added to the mixture and coated on polylactic acid surface and then films were irradiated at 0, 40 and 60 kGy doses of gamma-ray. Irradiation lowered the water solubility value of the films, furthermore 60 kGy dose reduced the water vapor permeability and tensile strength without significant effect on elongation at break of films. Results of release test indicated that gamma-ray decreased release rate of catechin from polymer matrix and generally release in high-fat simulant was about 2.5 times slower than low-fat simulant. This study was undertaken to design a packaging material with controlled release ability of antioxidant compound over time for further application in oxidation-sensitive foods.

Effects of heat treatment on the phenolic compounds and antioxidant capacity of quince fruit and its tisane's sensory properties.

The quince fruit can be an alternative for producing healthy tea because it is a rich source of bioactive compounds. This study was conducted to evaluate the effects of heating (for 20, 40 and 60 min) on the bioactive compounds of quince and quality attributes its tisanes. To this aim, phenolic content of the treated sample (for 40 min according to sensory analysis) was evaluated by HPLC and antioxidant activity was measured using DPPH method. The analysis of sensory evaluation data demonstrated that the heating process significantly improved the color and taste of the quince tisanes. Thermal treatment (180 °C) for 40 min had maximum acceptability. Although, results confirmed negative effects of the heating process on phenolic content, it showed an increase in antioxidant activity. The HPLC data displayed significant reduction in total amount of five identified phenolic compounds from 460.51 to 25.49 mg/25 g extract by heat treatment. In contrast the roasted sample exhibited lower IC50 (663.88 µg/ml) than the unprocessed sample (1098.66 µg/ml). Considering the elevated antioxidant activity achieved through the heating process, the quince tisane can be considered as a healthy beverage.

Physicochemical and morphological properties of resistant starch type 4 prepared under ultrasound and conventional conditions and their in-vitro and in-vivo digestibilities.

In the present work, cross-linked resistant starch (RS4) was prepared under sonication and conventional conditions at various levels of pH (9-12) and cross-linker concentration (sodium trimetaphosphate/sodium tripolyphosphate 99:1, 5-15%). It was found that phosphorous and resistant starch content was generally increased by increasing the cross-linker concentration, pH and application of sonication. The damage to the surface of sonicated granules was revealed by scanning electron micrographs. The presence of cross-linked phosphorous groups was demonstrated by FT-IR results through the appearance of a new peak at wave numbers of 1248-1252 cm-1 that was more conspicuous in sonicated cross-linked samples. Sonicated cross-linked starches showed higher gelatinization temperatures and lower degrees of crystallinity, while no changes was detected in terms of A-type crystalline pattern. The development of viscosity was diminished prominently by the extreme cross-linking reactions in both sonicated and conventional cross-linked starches. The least glycemic index value was obtained for sonicated cross-linked starches which was negatively correlated to their higher RS content measured in-vitro. These results provide novel information on the preparation of cross-linked resistant starch under sonication conditions.

Effect of active lipid-based coating incorporated with nanoclay and orange peel essential oil on physicochemical properties of Citrus sinensis.

The aim of this study was to evaluate the different lipid-based coating on the physicochemical properties and shelf life of blood orange. In this study, four different carnauba wax coatings formula were used: carnauba wax, carnauba wax incorporated with orange peel essential oil (OPEO) (1%), carnauba wax with montmorillonite nanoclay (MMT) (2%), and carnauba wax combination by OPEO (0.5%) and MMT (1%). Physicochemical properties (total phenol content, antioxidant activity, °Brix, titratable acidity, vitamin C, color, firmness, and pH) of fruits were determined throughout the storage. According to the results, carnauba wax with MMT was better than the other treatments. The highest antioxidant activity was observed in carnauba wax coating containing MMT and total phenol and DDPH gained 733.00 ± 1.204 (mg gallic acid/100 g) and 78.327 ± 0/364%, respectively, at 100th day. Blood orange coated by carnauba wax with MMT had the least of deformation and dissolved solid and the highest acidity rather than other treatments. Moreover, time storage and coating had significant effect on vitamin C content in which maximum and minimum amount was observed in wax coating incorporated by MMT and combination with MMT and OPEO treatments, respectively. Fruits coating with MMT showed better brightness.

The cell wall compound of Saccharomyces cerevisiae as a novel wall material for encapsulation of probiotics.

Yeast cell wall is known as a food grade ingredient which is recently being used increasingly as a novel coating for encapsulation of different materials in the food industry. This application is limited to core materials smaller than yeast in size. In this study, we have tried to encapsulate larger particles by crushing yeast cells. Hence, probiotic bacteria of Lactobacillus acidophilus and Bifidobacterium bifidum were encapsulated firstly by calcium alginate using the emulsion method and these microbeads were coated again by Saccharomyces cerevisiae cell wall compound and another layer of calcium alginate. The average diameter of microcapsules for single layer microbeads (M), microbeads coated by two layers of alginate (MCA), and microbeads coated by a layer of yeast cell and two layers of alginate (MCYA) were 54.25±0.18, 77.43±8.24 and 103.66±13.33µm, respectively. In simulated gastrointestinal conditions, there was a significant (P<0.05) enhancement in resistance of L. acidophilus when applying a layer of S. cerevisiae cell wall compound. For MCA and MCYA after 2h exposure to simulated gastric juice, it was revealed a log reduction of 1.53±0.1 and 1.1±0.02 with pH1.55 and in simulated intestinal juice, 2.92±0.04 and 2.42±0.06 with 0.6% bile after previous 1h incubation in gastric conditions, respectively. It can be concluded that the cell wall compound of S. cerevisiae is a suitable protective coating for probiotics and it can improve the survival of probiotics within food products.

Evaluation of folic acid release from spray dried powder particles of pectin-whey protein nano-capsules.

Our main goal was to evaluate release kinetics of nano-encapsulated folic acid within a double W1/O/W2 emulsion. First, W1/O nano-emulsions loaded with folic acid were prepared and re-emulsified into an aqueous phase (W2) containing single whey protein concentrate (WPC) layer or double layer complex of WPC-pectin to form W1/O/W2 emulsions. Final double emulsions were spray dried and their microstructure was analyzed in terms of scanning electron microscopy (SEM), and Fourier Transform Infrared spectroscopy (FTIR). Also the release trends of folic acid were determined and fitted with experimental models of zero and first order, Higuchi, and Hixson-Crowell. It was revealed that folic acid nano-capsules made with Span as the surfactant had the lowest release rate in acidic conditions (pH=4) and highest release in the alkaline conditions (pH=11). The best model fitting for folic acid release data was observed for single layer WPC encapsulated powders with the highest R2. Our FTIR data showed there was no chemical interaction between WPC and pectin in double layered capsules and based on SEM results, single WPC layered capsules resulted in smooth and uniform particles which by incorporating pectin, some wrinkles and shrinkage were found in the surface of spray dried powder particles.

Optimization of folic acid nano-emulsification and encapsulation by maltodextrin-whey protein double emulsions.

Due to susceptibility of folic acid like many other vitamins to environmental and processing conditions, it is necessary to protect it by highly efficient methods such as micro/nano-encapsulation. Our aim was to prepare and optimize real water in oil nano-emulsions containing folic acid by a low energy (spontaneous) emulsification technique so that the final product could be encapsulated within maltodextrin-whey protein double emulsions. A non ionic surfactant (Span 80) was used for making nano-emulsions at three dispersed phase/surfactant ratios of 0.2, 0.6, and 1.0. Folic acid content was 1.0, 2.0, and 3.0mg/mL of dispersed phase by a volume fraction of 5.0, 8.5, and 12%. The final optimum nano-emulsion formulation with 12% dispersed phase, a water to surfactant ratio of 0.9 and folic acid content of 3mg/mL in dispersed phase was encapsulated within maltodextrin-whey protein double emulsions. It was found that the emulsification time for preparing nano-emulsions was between 4 to 16 h based on formulation variables. Droplet size decreased at higher surfactant contents and final nano-emulsions had a droplet size<100 nm. Shear viscosity was higher for those formulations containing more surfactant. Our results revealed that spontaneous method could be used successfully for preparing stable W/O nano-emulsions containing folic acid.

Crocin loaded nano-emulsions: Factors affecting emulsion properties in spontaneous emulsification.

Spontaneous emulsification may be used for encapsulating bioactive compounds in food and pharmaceutical industry. It has several advantages over high energy and other low energy methods including, protecting sensitive compounds against severe conditions of high energy method and its ability to minimize surfactant, removal of cosurfactant and thermal stability compared with other low energy methods. In this study, we examined possibility of encapsulating highly soluble crocin in W/O micro-emulsions using spontaneous method which further could be used for making double emulsions. Nonionic surfactants of Span 80 and polyglycerol polyricinoleate (PGPR) were used for making micro-emulsions that showed the high potential of PGPR for spontaneous method. Surfactant to water ratio (SWR%) was evaluated to find the highest amount of aqueous phase which can be dispersed in organic phase. Droplet size decreased by increasing SWR toward the SWR=100% which had the smallest droplet size and then increased at higher levels of surfactant. By increasing SWR, shear viscosity increased which showed the high effect of PGPR on rheological properties. This study shows in addition to W/O micro-emulsions, spontaneous method could be used for preparing stable O/W micro-emulsions.

A mixture design approach to optimizing low cholesterol mayonnaise formulation prepared with wheat germ protein isolate.

The aim of this study was to optimize the mixture proportion of low cholesterol mayonnaise containing wheat germ protein isolate (WGPI) and xanthan gum (XG), as emulsifying agents in mayonnaise preparation. The mayonnaise prepared with different combinations of WGPI, egg yolk (0-9 % of each component) and XG (0-0.5 %). The optimized mixture proportions of low cholesterol mayonnaise were determined by applying the optimal mixture design method to acquire the mayonnaise with proper stability, texture, rheological properties and sensory scores. Optimum values of WGPI, XG and egg yolk in the mixture were found to be 7.87 %, 0.2 % and 0.93 %, respectively (of 9 % egg yolk). The WGPI, due to unique functional properties, had the greatest effect on properties of mayonnaise samples. Moreover, combination of XG and WGPI, improved the stability, heat stability, viscosity, consistency coefficient and textural properties of product. However, the overall acceptance was maximum in a mixture contained high amount of WGPI and XG and low amount of egg yolk. The results of this research showed the feasibility of preparation a low cholesterol mayonnaise by application a desirable combination of WGPI, XG, and egg yolk, with comparable properties those of the conventional mayonnaise.

Enhanced biomass delignification and enzymatic saccharification of canola straw by steam-explosion pretreatment.

BACKGROUND: In recent decades, bioconversion of lignocellulosic biomass to biofuel (ethanol and biodiesel) has been extensively investigated. The three main chemical constituents of biomass are cellulose, hemicellulose and lignin. Cellulose and hemicellulose are polysaccharides of primarily fermentable sugars, glucose and xylose respectively. Hemicellulose also includes small fermentable fractions of arabinose, galactose and mannose. The main issue in converting lignocellulosic biomass to fuel ethanol is the accessibility of the polysaccharides for enzymatic breakdown into monosaccharides. This study focused on the use of steam explosion as the pretreatment method for canola straw as lignocellulosic biomass. RESULTS: Result showed that steam explosion treatment of biomass increased cellulose accessibility and it hydrolysis by enzyme hydrolysis. Following 72 h of enzyme hydrolysis, a maximum cellulose conversion to glucose yield of 29.40% was obtained for the steam-exploded sample while the control showed 11.60% glucose yields. Steam explosion pretreatment increased glucose production and glucose yield by 200% and 153.22%, respectively, compared to the control sample. The crystalline index increased from 57.48% in untreated canola straw to 64.72% in steam-exploded samples. CONCLUSION: Steam explosion pretreatment of biomass increased cellulose accessibility, and enzymatic hydrolysis increased glucose production and glucose yield of canola straw.

Soluble soybean polysaccharide: a new carbohydrate to make a biodegradable film for sustainable green packaging.

Biodegradable edible films based on soluble soybean polysaccharide (SSPS), a new film-forming material, and three levels of glycerol (20%, 30% and 40%, w/w) as plasticizer, were developed and evaluated in terms of physical, mechanical, barrier and optical properties as well as their microstructure. SSPS-based films with a concentration of 20% glycerol possessed the lowest water vapor permeability. Increasing the glycerol content increased (P<0.05) values for elongation at break, but decreased (P<0.05) tensile-strength values. It was found that plasticizer concentration significantly affected the films' glass-transition temperature; however, it had no significant effect on their melting point. Color measurement showed that increasing the glycerol concentration caused the b and L values to increase, while ΔE value decreased. These results were explained by the film's microstructure, which was analyzed by atomic force microscopy and scanning electron microscopy. The results indicated that SSPS could be a promising food-packaging material.