ABSTRACT
BACKGROUND: Alzheimer's disease is a devastative neurodegenerative disorder. To date, there has been no animal model that could unravel the complete disease pathology. Magnetic resonance imaging has played a pivotal role in the quantitative assessment of brain tissue atrophy for a few decades. In particular, temporal lobe atrophy and ventricular dilatation have been found to be sensitive in Alzheimer's disease. METHODS: The present study focused on the replication of these crucial pathological events to enable disease progression to be diagnosed at an early stage and stopped through the use of potential therapeutic strategies. RESULT: The objective of this study was to show temporal lobe atrophy and ventricular dilatation in aluminium maltolate-treated aged New Zealand rabbit, and our study was able to demonstrate this for the first time. CONCLUSION: The present study makes this animal model a substantial one for further molecular level studies and opens up new targets for potential therapeutic strategies.
Subject(s)
Alzheimer Disease/chemically induced , Magnetic Resonance Imaging/methods , Neurotoxins/toxicity , Organometallic Compounds/toxicity , Pyrones/toxicity , Temporal Lobe/pathology , Alzheimer Disease/metabolism , Alzheimer Disease/pathology , Animals , Atrophy/pathology , Disease Models, Animal , Female , Humans , RabbitsABSTRACT
BACKGROUND: Alzheimers disease (AD) is a devastative neurodegenerative disorder. Lack of substantial animal model that can unravel molecular underpinnings has been a major lacuna which limited the understanding of the etiology of the disease in turn limiting the employment of potential therapeutic strategies to combat the disease for a few decades. Our studies for the first time provided substantial animal model and tattered the etiology of the disease at a molecular level. METHODS: In this study DNA was isolated from Hippocampus (H), Midbrain (M) and Frontal Cortex (Fc) of control and aluminium maltolate (Al-M) treated aged New Zealand rabbit brain. DNA damage has been studied using Agarose gel electrophoresis, Ethidium Bromide (EtBr) binding and Melting temperature techniques. RESULTS: Al-M treated aged New Zealand rabbit's H and M showed higher DNA damage compared to corresponding controls, where as Fc showed mild DNA damage compared to corresponding controls. CONCLUSIONS: This study tangibly provides substantial molecular level understanding of the disease in turn providing an adequate platform to streamline potential therapeutic strategies. KEYWORDS: Alzheimer's disease; Aluminium maltolate; Animal model; DNA damage.
