ABSTRACT
Current resources to support genetic screening among the Uygur population in Xinjiang territory for atrial fibrillation (AF) have not been well established and large-scale epidemiological analyses are needed. Using patients from the Xinjiang Uygur population as subjects, and the delayed rectifier potassium channel KCNE1 and its associated polymorphism G38S (rs1805127) as the candidate gene, we analyzed the correlation between the G38S polymorphism and AF among the senior Uygur population in Xinjiang Province. Peripheral blood from AF Uygur patients (patient group) or non-AF Uygur patients (control group) from Xinjiang territory was collected (70 patients each). DNA was purified and tested by polymerase chain reaction-restriction fragment length polymorphism for the genotype and allelic distribution of KCNE1 (G38S). Correlation analysis between AF and multiple health-related factors was performed by logistic regression. Among patients with the KCNE1 G38S polymorphism, the genotypes AA, AG, and GG were present at frequencies of 17.14, 27.14, and 55.71%, respectively, in the patient group, compared with 24.29, 50, and 25.71%, respectively, in the control group. The difference between these two groups was shown to be statistically significant (P < 0.05), and the frequency of the G allele was significantly higher in the patient group (P < 0.05). Logistic regression showed that the GG genotype is correlated with the incidence of AF in Uygur seniors (P < 0.05). The incidence of AF among the senior Uygur population in Xinjiang territory was correlated with the KCNE1 (G38S) polymorphism, which may be an independent risk factor for Uygur AF patients.
Subject(s)
Alleles , Atrial Fibrillation/epidemiology , Atrial Fibrillation/genetics , Ethnicity/genetics , Genetic Association Studies , Genetic Predisposition to Disease , Polymorphism, Single Nucleotide , Potassium Channels, Voltage-Gated/genetics , Aged , Aged, 80 and over , Atrial Fibrillation/physiopathology , Case-Control Studies , China/epidemiology , Comorbidity , Female , Gene Frequency , Genotype , Humans , Male , Middle Aged , Odds RatioABSTRACT
The aim of this study was to explore the changes in gene and protein expressions of tyrosine hydroxylase (TH) and growth-associated protein 43 (GAP43) in aging atrial fibrillation patients of Xinjiang Uygur and Han nationality, and the significance of the changes. Real-time polymerase chain reaction and Western blot analysis were used to detect gene and protein expressions of TH and GAP43 in atrial tissues of 54 patients with valvular heart disease. mRNA and protein expressions of GAP43 and TH were significantly different between the sinus rhythm and atrial fibrillation groups (P < 0.05). Protein expressions of GAP43 and TH of both nationalities differed significantly between the sinus rhythm group and the atrial fibrillation group (P < 0.05), whereas there was no statistical difference between the two nationalities within each group (P > 0.05). Protein expressions of GAP43 and TH differed significantly among different age groups of different nationalities in the sinus rhythm and atrial fibrillation groups (P < 0.05); only protein expression of GAP43 differed significantly in different age groups in the atrial fibrillation group (P < 0.05). The changes of mRNA and protein expressions of TH and GAP43 played a vital role in the process of maintaining the atrial fibrillation. Therefore, increased expression of TH and GAP43 might be a molecular mechanism for left atrial myoelectricity remodeling of aging atrial fibrillation patients, which might be potential therapeutic targets of atrial fibrillation.
