ABSTRACT
Particles with the characteristic shape of enveloped retroviral particles and maximal specific reverse transcriptase (RTase) activity at buoyant density of 1.15-1.17 g/ml have been isolated from human first-trimester chorionic villous tissue. Murine monoclonal antibodies (mAbs) to these isolated particles were generated. One IgM mAb (RV3-27) showed granular staining of cytoplasmic structures within syncytiotrophoblast by immunohistochemistry. Immunoelectron microscopic studies have demonstrated focal localisation to small submembranous regions of syncytiotrophoblast, as well as reaction with detergent-disrupted isolated placental retroviral-like particles. The RV3-27 mAb did not stain other human tissues in this focal manner, although increased generalised cytoplasmic staining was not uncommon; also, this mAb did not react strongly with the surface or cytoplasm of a variety of human cell lines (including choriocarcinoma cells). Immunoblotting and HPLC analyses have indicated the reactive placental antigen to be a 17-25 kDa protein. It is suggested that the RV3-27 mAb may be reactive with a syncytiotrophoblast antigen encoded by an endogenous retroviral sequence.
Subject(s)
Antibodies, Monoclonal/immunology , Antibodies, Viral/immunology , Chorionic Villi/virology , Retroviridae/isolation & purification , Trophoblasts/immunology , Animals , Choriocarcinoma/pathology , Choriocarcinoma/virology , Extraembryonic Membranes/immunology , Female , Humans , Immunoglobulin M/immunology , Immunologic Techniques , Mice , Mice, Inbred BALB C , Microscopy, Electron , Neoplasms/pathology , Organ Specificity , RNA-Directed DNA Polymerase/analysis , Retroviridae/enzymology , Retroviridae/immunology , Retroviridae/ultrastructure , Trophoblasts/virology , Tumor Cells, Cultured , Uterine Neoplasms/pathology , Uterine Neoplasms/virology , Viral Proteins/analysisABSTRACT
When growth cartilage from rachitic chicks was cultured in the presence of the calcium-regulating hormone 1,25-dihydroxyvitamin D3 (1,25-(OH)2D3), collagen resorption was increased and collagen synthesis decreased compared to control cultures containing no hormone. The minimum concentration of the hormone that caused a statistically significant inhibition of collagen synthesis was 10(-8) mol/l. Collagen synthesis by growth cartilage from normal chicks was also reduced by 1,25-(OH)2D3, showing that it was not an abnormal response of vitamin D-depleted tissue. 25-Hydroxyvitamin D3 and 24,25-dihydroxyvitamin D3 also inhibited collagen synthesis by cultures of growth cartilage but only at higher metabolite concentrations. 1,25-Dihydroxyvitamin D3 (10(-7) mol/l) did not significantly inhibit collagen synthesis by cultures of articular fibrocartilage and of sternal cartilage, tissues that do not calcify physiologically. The minimum concentration of 1,25-(OH)2D3 (10(-9) mol/l) necessary to cause decreased collagen synthesis by embryonic chick calvaria was lower than the value obtained with growth cartilage; this suggests that bone cells may be more sensitive to the hormone in this respect than are growth cartilage chondrocytes. These findings provide evidence of a direct role of 1,25-(OH)2D3 in the control of endochondral bone formation which is consistent with its primary role in the maintenance of plasma calcium homeostasis.
Subject(s)
Cartilage/metabolism , Collagen/biosynthesis , Vitamin D Deficiency/metabolism , Vitamin D/metabolism , Animals , Calcitriol/metabolism , Cartilage/growth & development , Chickens , Dihydroxycholecalciferols/metabolism , Male , Organ Culture Techniques , Skull/metabolismABSTRACT
To determine the factors affecting cigarette smoking status after the development of ischemic heart disease, 200 patients were studied who were hospitalized with a coronary event and enrolled in a cardiac rehabilitation program. There were significantly more current smokers (55%) among the 96 patients hospitalized with an initial presentation of a coronary event as compared to the percent of current-smokers (34%) among the 104 patients hospitalized with a recurrent coronary event (p less than 0.01). In addition to the occurrence of a prior event, increasing age also significantly discriminated ex-cigarette smokers from current smokers. Among the patients with a recurrent event ex-smokers (44%) and current smokers (34%) differed significantly with respect to age, education, occupation, negative attitudes towards smoking and peak number of cigarettes smoked. Two models were developed which were able to correctly classify 61.7 and 69.1% of the patients with regard to smoking status.