ABSTRACT
In the search for effective methods of biological control of stored products insect-pests, the essential oil of Origanum vulgaris was extracted using Clevenger apparatus and tested on reproduction and mortality of flour moth Ephestia kuehniella. Bio-insecticide works with a double action mechanism; administered by inhalation in pupae, essential oil affects their pupal development. It also disrupts the reproduction of exuviated adults by extending the preoviposition period and reducing the period of egg laying and fecundity because fecundated females cannot live more than four days compared to control group. Moreover, the essential oil administered by inhalation in adults causes a significant mortality rate compared to control group by reducing their lifespan. A repellent effect against larvae and adults of this pest was noticed in this work. This allowed us ranking this oil as "moderately repulsive".
Subject(s)
Flour/parasitology , Food Preservation/methods , Insecticides/toxicity , Moths/drug effects , Oils, Volatile/toxicity , Origanum/chemistry , Animals , Female , Fertility/drug effects , Insecticides/chemistry , Insecticides/isolation & purification , Male , Moths/physiology , Oils, Volatile/chemistry , Oils, Volatile/isolation & purification , Reproduction/drug effectsABSTRACT
Objective To investigate the antioxidant and hepatoprotective properties of Juniperus phoenicea (J. phoenicea) berries against CCl
ABSTRACT
OBJECTIVE@#To investigate the antioxidant and hepatoprotective properties of Juniperus phoenicea (J. phoenicea) berries against CCl-induced oxidative damage in rats.@*METHODS@#Hepatotoxicity was induced in albino Wistar rats by single dose of CCl dissolved in olive oil (1 mL/kg BW, 1/1 in olive oil, i.p.). Aqueous extract of J. phoenicea berries (AEJP) was administered at the dose of 250 mg/kg/day by gavage for 12 days.@*RESULTS@#Obtained results revealed that administration of CCl caused a significant increase in plasma ASAT, ALAT, ALP and LDH activities and total bilirubin concentration, compared to the control group. While, albumin and total protein concentration were significantly lower. Additionally, a significant decrease in the level of hepatic GSH, GPx and GST activities associated with a significant increase of MDA content in CCl group than those of the control. However, the treatment of experimental rats with AEJP prevented these alterations and maintained the antioxidant status. The histopathological observations supported the biochemical evidences of hepatoprotection.@*CONCLUSIONS@#The results of the present investigation indicate that J. Phoenicea possesses hepatoprotective activity and this effect was may be due to its antioxidant properties.
ABSTRACT
Deltamethrin is a synthetic pyrethroid insecticide. It is known for its wide toxic manifestations. The present experiment pertains to the protective role of vitamin C against haematological and biochemical toxicity induced by deltamethrin during 4 weeks. Male Wistar rats were divided into four groups of eight each: Group I served as control rats; Group II received deltamethrin (1.28 mg/kg BW) in drinking water. Group III received both deltamethrin and vitamin C (200mg/kg BW; by i.p. injection); Group IV received vitamin C (200mg/kg BW). Exposure of rats to deltamethrin caused significant changes of some haematological parameters (red blood cells (RBC), haemoglobin (Hb), haematocrit (Ht), mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), mean corpuscular haemoglobin concentration (MCHC), platelet (Plt) and white blood cells (WBC)) in treated rats compared to controls. Significant increases in the levels of hepatic markers enzymes (alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), γ-Glutamyl transpeptidase (γ-GT)). Furthermore, renal markers such as urea and creatinine were increased in deltamethrin treated rats. Additionally, serum cholesterol and lipid peroxidation were significantly enhanced. Co-administration of vitamin C to the group III restored all the parameters cited above to near-normal values. Therefore, our investigation revealed that vitamin C appeared to be a promising agent for protection against deltamethrin-induced toxicity.
