Determination of heavy metals in selected fish species and seawater from the South Durban Industrial Basin, KwaZulu-Natal, South Africa.

The South Durban Industrial Basin (SDIB), South Africa, an economic hub that spans a long coastline, is a disaster management hotspot, which threatens the sustainability of the fishing industry in this region. This study investigated the elemental concentrations in fish and seawater from a mini-ecosystem in the SDIB, to determine if a direct relationship exists between these two matrices, and to assess for metal toxicities. The results showed As and Cr to be above the maximum permissible limits (MPLs) of the World Health Organization (WHO) for all fish species (blacktail, karanteen, five finger, mullet, and pompano), while fish from Isipingo Beach exceeded the WHO MPLs for Pb, As, and Cr. Fish did not display signs of metal toxicity indicating either a harmless form of metals or high tolerance levels. Typical concentrations of metals in seawater (mg L-1) at (Isipingo Beach, Cuttings Beach, and Amanzimtoti Beach) were found to be for Al (3, 2.7, and 16.4), As (0.17, 0.11, and 0.19), Cr (0.16, 0.17, and 0.23), Cu (0.15, 0.13, and 0.24), Fe (2.7, 3.45, and 33.9), and Zn (2, 2.1, and 2.12). Except for As, estimated daily intakes, target hazard quotients, and carcinogenic risks indicated no associated health risks due to consumption of the fish from the SDIB. However, due to target hazard quotients and carcinogenic risks for As being above 4 and 0.0001, respectively, fish from the SDIB should be consumed in moderation or not consumed to prevent long-term toxic effects of As.

Heavy metal distribution in Laportea peduncularis and growth soil from the eastern parts of KwaZulu-Natal, South Africa.

Laportea peduncularis is a medicinal plant consumed by the native communities in South Africa. Due to its oral consumption, its potential for harming the human health and the distribution of metals in the leaves of L. peduncularis as a function of soil characteristics were evaluated. Broadly, the concentrations of metals in the soil were in decreasing order of Fe > Ca > Mg > Mn > Zn > Cr > Cu > Ni > As > Co > Cd > Pb. Low-molecular-weight organic acid, calcium chloride, and ethylenediaminetetraacetic acid extraction methods were employed to assess for exchangeable forms of metals in the soil. Geoaccumulation indices and enrichment factors showed no contamination or enrichment for most of the heavy metals studied except for Cd, which showed moderate contamination and significant enrichment at Mona, KwaZulu-Natal. Principal component and cluster analyses revealed that As, Cd, Fe, and Ni in the soil came from the same source, whilst Cu, Pb, and Zn in the soil were from a common origin. Correlation analysis showed significantly positive correlation between heavy metals As, Cd, Fe, and Ni in the soil, as well as between Cu, Pb, and Zn, confirming the metals' common origin. Concentrations of metals in plants and soil were influenced by site, but the availability and uptake of the metals solely depended on the plant's inherent controls.

The distribution of macronutrients, anti-nutrients and essential elements in nettles, Laportea peduncularis susp. peduncularis (River nettle) and Urtica dioica (Stinging nettle).

Laportea peduncularis and Urtica dioica, which are popularly known as "Nettles" belong to the plant family Urticaceae and are consumed as green vegetables or used for their medicinal benefit in many countries in Africa, Asia, Europe and America. This study aimed at investigating the effect of cooking on the macronutrient, anti-nutrient and elemental composition of L. peduncularis and U. dioica leaves. The results showed a decrease in the crude fat, ash, carbohydrate and vitamin C content with cooking, but an increase in the vitamin E content. The anti-nutrient content (cyanides, phytates and saponins) increased slightly with cooking, while the oxalate content has decreased. The concentration of essential elements in cooked L. peduncularis leaves were found to be in decreasing order of Ca > Mg > Fe > Mn > Zn > Cu > Cr > Ni > Co. Both raw and cooked leaves of nettles were found to be rich sources of macronutrients and essential elements and may be used as alternatives to commercially available nutrient supplements. Statistical analyses (principal component analysis and correlations) indicated that certain elements taken up by these plants were from common sources. Both positive and negative relationships between nutrients, anti-nutrients and elements were observed in the plant leaves.