Isolation, identification and biological characterization of bacterial endophytes isolated from Gunnera Perpensa L.

In the present study, eleven endophytic bacterial strains, Herbaspirillum sp. (GP-SGM1, GP-SGM2, GP-SGM3, GP-SGM11), Pseudomonas sp. (GP-SGM4, GP-SGM5), Novosphingobium sp. GP-SGM6, Chryseobacterium sp. GP-SGM7, Labedella sp. GP-SGM8, Brevibacterium sp. GP-SGM9, and Pseudomonas sp. GP-SGM10, were isolated from the rhizomes of Gunnera perpensa L. The growth kinetics, assessed through maximum growth rates (µmax) and optical density (OD) values, revealed that GP-SGM7 exhibited highest µmax values of 0.33 ± 0.01 hr-1 with an OD of 4.20 ± 0.04. In contrast, GP-SGM11 exhibited the lowest µmax of 0.12 ± 0.05 hr-1 and the smallest OD of 1.50 ± 0.00. In addition, the endophyte crude extracts were tested for antibacterial activity against five pathogenic strains using the disk diffusion method, with GP-SGM7 crude extracts exhibiting promising antibacterial activity against Klebsiella pneumoniae, and Staphylococcus aureus. Antioxidant activity was determined by DPPH (2, 2-diphenyl-1-picrylhydrazyl) and FRAP (ferric reducing antioxidant power) assays. The crude extracts of GP-SGM1, GP-SGM7, GP-SGM9, and GP-SGM10 were the most effective at scavenging DPPH radicals, with GP-SGM7 also exhibiting high FRAP value of 0.54 ± 0.01. These findings emphasize the therapeutic potential of endophytic bacteria from Gunnera perpensa L. in addressing skin-related issues, including bacterial infections and free radicals.

Draft genome sequence of Pantoea ananatis strain MHSD5 isolated from surface sterilized leaves of medicinal plant, Pellaea calomelanos obtained in South Africa.

Pantoea ananatis strain MHSD5 is a bacterial endophyte isolated from the surface sterilized leaves of Pellaea calomelanos, which is a medicinal plant obtained in Limpopo province of South Africa. We present here the draft genome sequence and annotation of P. ananatis strain MHSD5. The genome assembly was 4.6 Mb in size with an N50 of 550,557 bp. A total of 4,350 putative protein coding sequence genes were predicted with PGAAP. This is the first draft genome of a bacterial endophyte symbiotically associated with P. calomelanos. This Whole Genome Shotgun project has been deposited at DDBJ/ENA/GenBank under the accession PUEK00000000. The version described in this paper is version PUEK01000000.