Determination of Atropine by HPLC in Plant of Datura by Liquid-Liquid Extraction and Magnet Solid-Phase Extraction.

Atropine is a tropane alkaloid found in abundance in Datura plant. We used two liquid-liquid extraction methods and magnet solid-phase extraction to compare the amount of atropine in these two types of plants (Datura innoxia and Datura stramonium). The surface magnetic nanoparticle Fe3O4 correction with an amine and dextrin, and finally, magnetic solid-phase extraction Fe3O4@SiO2-NH2-dextrin (MNPs-dextrin), was prepared. We determined the effect of significant parameters in the removal step and optimization of atropine measurements with half-fractional factorial design (25-1) and response surface methodology via central composite design. The optimum conditions are for desorption solvent = 0.5 mL methanol and desorption time of 5 min. We obtained an extraction recovery of 87.63% with a relative standard deviation of 4.73% via six frequented measurements on a 1 µg L-1 atropine standard solution based on the optimum condition. Preconcentration factors for MNPs are 81, limit of detection = 0.76 µg L-1 and limit of quantitation = 2.5 µg L-1.

Fe3 O4 and bimetal-organic framework Zn/Mg composite peroxide-like catalyze luminol chemiluminescence for specific measurement of atropine in Datura plant.

Atropine (AT) is an anticholinergic drug. AT is abundantly in Datura plant seeds. Fe3 O4 @Zn/Mg MOF (Fe3 O4 @MOF) composite was synthesized. The compound had a high peroxidase-like activity in a chemiluminescence (CL) reaction. Addition of AT quenched CL. The linear range and limit of detection were 5-600 µg L-1 and 2 × 10-2 µg L-1 . This method is fast, reversible, and selective, without biodegradability effects, high accuracy, and precision for measuring AT in the Datura plant.

Modification of bimetal Zn/ Mg MOF with nanoparticles Fe3O4 and Fe3O4@SiO2, investigation of the peroxidase-like activity of these compounds by calorimetry and fluorimetry methods.

In this article; the bimetal metal-organic framework Zn/Mg (Zn/Mg MOF) is synthesized. Then Zn/Mg MOF bimetal was combined with Fe3O4 and Fe3O4@SiO2, and composites of Fe3O4@ SiO2/MOF/Dextrin, Fe3O4@SiO2/MOF, Fe3O4@MOF/Dextrin and Fe3O4@MOF made. The peroxidase-like activity of these compounds was investigated and compared by calorimetric Resazurin (Rz) and O-phenylenediamine (OPD); (Rz-H2O2, OPD-H2O2) and fluorimetric Rz and terephtalic acid (TA); (Rz-H2O2, TA-H2O2). The Fe3O4@ MOF/Dextrin composite has the highest peroxidase-like activity. The effect factors (amount of pH (6), the values of TA (1.37 mM), H2O2 (0.025 mM), reaction time (8.15 min), and amount of Composite (116.67 mg)) to increase the catalytic activity of Fe3O4@ MOF/Dextrin measured by chemometrics method. The most suitable linear range of the calibration curve by the TA-H2O2 -Composite fluorimetric method is 1-600 µg L-1, and the detection limit is 2.27 µg L-1. The relative standard deviation (RSD%) for measuring concentration atropine 1 µg L-1 (n = 6) is 1.18%. Finally, from this system for measuring atropine extracted by the Liquid-liquid extraction (LLE) method in two types of plants, D. Innoxia north and west and D. stramonium north and west of Iran (118.25 µg L-1, 79.80 µg L-1) and (18.477 µg L-1, 9.27 µg L-1) used, respectively.