Avaliação da viabilidade do sêmen bubalino congelado pelos métodos convencional e automatizado (resultados parciais) / Evaluation of viability the buffalo semen frozen by conventional and automated methods (partial results)

In the Artificial Insemination it is essential to the use of frozen semen, which causes damage to thestructure of sperm. To avoid these cellular damage, there is a need to assess the viability of frozen semen buffaloby conventional and automated methods, and to predict which of the methods retrieve highest number of viablecells post-thawing. The aim of this study was to evaluate the efficiency of these two methods in buffalo semenfreezing. Semen was obtained from buffalo breeding and diluted in TES-TRIS. After semen freezing, the sampleswere evaluated for motility and vigor. There was no difference between the automated and conventionalmethods, respectively, for motility (67,5%±10 and 69,37±9,28), and the vigor (3,06±0,57 and 3,06±0,68).Therefore, it is concluded that the freezing methods are effective in cryopreservation the semen buffalo, however,it is suggested that more specific tests are performed to validate the protocols. (AU)

Associação entre o diâmetro folicular, no momento da retirada do implante de progesterona, e a taxa de ovulação de vacas após protocolo de sincronização / Association between follicular diameter at the time of removal of the progesterone and ovulation rate of cows after to the synchronization of ovulation protocol

The experiment was conducted at the Center of Biotechnology and Animal Reproduction (CEBRANUFPa),in the municipality of Castanhal, Pará State. The herd consisted of 24 crossbred cows with bodycondition score ranging between 2.5 and 3.5, divided randomly into 2 groups (G1 and G2) which were managedwith different protocols. G1 (D0-Be+P4 implant; D7-Pgf2; D9-measurement of the largest follicle + CE + eCG;D11- new measurement of FD and D15- verification of the presence or absence of CL and its measurement) G2(D0-Be + P4 implant, removal of implant D8 + FSH; D9- BE; D10-measurement of FD; D14- verification of thepresence of CL). Therefore, is possible the identification and selection of animals subjected to thesynchronization protocol for IATF.(AU)

Comparação do diâmetro do folículo dominante no momento da IATF e do corpo lúteo formado, em diferentes protocolos de sincronização da ovulação em vacas mestiças / Comparison of the diameter of the dominant follicle at the time of TAI and the corpus luteum formed in different synchronization protocols of ovulation in crossbred cows

The beef cattle industry in Brazil is characterized as an activity that goes through a period of growth.The experiment was conducted at the Center of Animal Reproduction Biotechnology (CEBRAN-UFPA), in themunicipality of Castanhal, Pará State. The herd consisted of 24 crossbred cows with body condition scoreranging between 2.5 and 3, 5, divided randomly into 2 groups (G1 and G2) which were managed with differentprotocols. G1 (D0-Be+P4 implant; D7-Pgf2; D9-measurement of the largest follicle + CE + eCG; D11- newmeasurement of FD and D15- verification of the presence or absence of CL and its measurement) G2 (D0-Be +P4 implant, removal of implant D8 + FSH; D9- BE; D10-measurement of FD; D14- verification of the presenceof CL). In the present study was not observed statistical difference for the size of follicular diameter at themoment of TAIF and CL formed in different synchronization protocols.(AU)

Associação entre o diâmetro folicular, no momento da retirada do implante de progesterona, e a taxa de ovulação de vacas após protocolo de sincronização / Association between follicular diameter at the time of removal of the progesterone and ovulation rate of cows after to the synchronization of ovulation protocol

The experiment was conducted at the Center of Biotechnology and Animal Reproduction (CEBRANUFPa),in the municipality of Castanhal, Pará State. The herd consisted of 24 crossbred cows with bodycondition score ranging between 2.5 and 3.5, divided randomly into 2 groups (G1 and G2) which were managedwith different protocols. G1 (D0-Be+P4 implant; D7-Pgf2; D9-measurement of the largest follicle + CE + eCG;D11- new measurement of FD and D15- verification of the presence or absence of CL and its measurement) G2(D0-Be + P4 implant, removal of implant D8 + FSH; D9- BE; D10-measurement of FD; D14- verification of thepresence of CL). Therefore, is possible the identification and selection of animals subjected to thesynchronization protocol for IATF.

Comparação do diâmetro do folículo dominante no momento da IATF e do corpo lúteo formado, em diferentes protocolos de sincronização da ovulação em vacas mestiças / Comparison of the diameter of the dominant follicle at the time of TAI and the corpus luteum formed in different synchronization protocols of ovulation in crossbred cows

The beef cattle industry in Brazil is characterized as an activity that goes through a period of growth.The experiment was conducted at the Center of Animal Reproduction Biotechnology (CEBRAN-UFPA), in themunicipality of Castanhal, Pará State. The herd consisted of 24 crossbred cows with body condition scoreranging between 2.5 and 3, 5, divided randomly into 2 groups (G1 and G2) which were managed with differentprotocols. G1 (D0-Be+P4 implant; D7-Pgf2; D9-measurement of the largest follicle + CE + eCG; D11- newmeasurement of FD and D15- verification of the presence or absence of CL and its measurement) G2 (D0-Be +P4 implant, removal of implant D8 + FSH; D9- BE; D10-measurement of FD; D14- verification of the presenceof CL). In the present study was not observed statistical difference for the size of follicular diameter at themoment of TAIF and CL formed in different synchronization protocols.

Avaliação da viabilidade do sêmen bubalino congelado pelos métodos convencional e automatizado (resultados parciais) / Evaluation of viability the buffalo semen frozen by conventional and automated methods (partial results)

In the Artificial Insemination it is essential to the use of frozen semen, which causes damage to thestructure of sperm. To avoid these cellular damage, there is a need to assess the viability of frozen semen buffaloby conventional and automated methods, and to predict which of the methods retrieve highest number of viablecells post-thawing. The aim of this study was to evaluate the efficiency of these two methods in buffalo semenfreezing. Semen was obtained from buffalo breeding and diluted in TES-TRIS. After semen freezing, the sampleswere evaluated for motility and vigor. There was no difference between the automated and conventionalmethods, respectively, for motility (67,5%±10 and 69,37±9,28), and the vigor (3,06±0,57 and 3,06±0,68).Therefore, it is concluded that the freezing methods are effective in cryopreservation the semen buffalo, however,it is suggested that more specific tests are performed to validate the protocols.