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1.
Chromosoma ; 118(4): 471-86, 2009 Aug.
Article in English | MEDLINE | ID: mdl-19396455

ABSTRACT

The Mre11-Rad50-Nbs1 (MRN) complex is required for numerous cellular processes that involve interactions with DNA double-strand breaks. For the majority of these processes, the MRN complex is thought to act as a unit, with each protein aiding the activity of the others. We have examined the relationship between Mre11 and Rad50 during meiosis in the basidiomycete Coprinus cinereus (Coprinopsis cinerea), investigating to what extent activities of Mre11 and Rad50 are interdependent. We showed that mre11-1 is epistatic to rad50-1 with respect to the time of meiotic arrest, indicating that Mre11 activity facilitates the diffuse diplotene arrest of rad50 mutants. Anti-Mre11 and anti-Rad50 antibodies were used to examine MRN complex localization in a wild-type strain and in spo11, mre11, and rad50 mutants. In wild type, numbers of Mre11 and Rad50 foci peaked at time points corresponding to leptotene and early zygotene. In the spo11-1 mutant, which is defective in meiotic double-strand break formation, foci accumulated throughout prophase I. Of seven MRN mutants examined, only two rad50 strains exhibited Mre11 and Rad50 foci that localized to chromatin, although Mre11 protein was found in the cell for all of them. Analysis of predicted mutant structures showed that stable localization of Mre11 and Rad50 does not depend upon a wild-type hook-proximal coiled coil, but does require the presence of the Rad50 ATPase/adenylate cyclase domains. We found that Mre11 and Rad50 were interdependent for binding to meiotic chromosomes. However, the majority of foci observed apparently contained only one of the two proteins. Independent Mre11 and Rad50 foci might indicate disassociation of the complex during meiosis or could reflect independent structural roles for the two proteins in meiotic chromatin.


Subject(s)
Coprinus/metabolism , DNA-Binding Proteins/metabolism , Endodeoxyribonucleases/metabolism , Exodeoxyribonucleases/metabolism , Fungal Proteins/metabolism , Mutation , Chromosomes, Fungal/metabolism , Coprinus/enzymology , Coprinus/genetics , DNA-Binding Proteins/genetics , Endodeoxyribonucleases/genetics , Epistasis, Genetic , Exodeoxyribonucleases/genetics , Fluorescent Antibody Technique , Fungal Proteins/genetics , Immunoblotting , Meiosis , Meiotic Prophase I , Protein Binding
2.
Genetics ; 180(4): 1889-907, 2008 Dec.
Article in English | MEDLINE | ID: mdl-18940790

ABSTRACT

The Mre11/Rad50/Nbs1 (MRN) complex is required for eukaryotic DNA double-strand break (DSB) repair and meiotic recombination. We cloned the Coprinus cinereus rad50 gene and showed that it corresponds to the complementation group previously named rad12, identified mutations in 15 rad50 alleles, and mapped two of the mutations onto molecular models of Rad50 structure. We found that C. cinereus rad50 and mre11 mutants arrest in meiosis and that this arrest is Spo11 dependent. In addition, some rad50 alleles form inducible, Spo11-dependent Rad51 foci and therefore must be forming meiotic DSBs. Thus, we think it likely that arrest in both mre11-1 and the collection of rad50 mutants is the result of unrepaired or improperly processed DSBs in the genome and that Rad50 and Mre11 are dispensable in C. cinereus for DSB formation, but required for appropriate DSB processing. We found that the ability of rad50 mutant strains to form Rad51 foci correlates with their ability to promote synaptonemal complex formation and with levels of stable meiotic pairing and that partial pairing, recombination initiation, and synapsis occur in the absence of wild-type Rad50 catalytic domains. Examination of single- and double-mutant strains showed that a spo11 mutation that prevents DSB formation enhances axial element (AE) formation for rad50-4, an allele predicted to encode a protein with intact hook region and hook-proximal coiled coils, but not for rad50-1, an allele predicted to encode a severely truncated protein, or for rad50-5, which encodes a protein whose hook-proximal coiled-coil region is disrupted. Therefore, Rad50 has an essential structural role in the formation of AEs, separate from the DSB-processing activity of the MRN complex.


Subject(s)
Coprinus/genetics , Fungal Proteins/genetics , Meiosis/genetics , Mutation , Recombination, Genetic/genetics , Synaptonemal Complex/metabolism , Alleles , Coprinus/metabolism , DNA Repair , DNA, Fungal/metabolism , Fungal Proteins/metabolism , Microscopy, Electron , Spores, Fungal/growth & development , Synaptonemal Complex/genetics , Synaptonemal Complex/ultrastructure
3.
J Mol Biol ; 374(2): 399-410, 2007 Nov 23.
Article in English | MEDLINE | ID: mdl-17931656

ABSTRACT

Nucleic acid polymers selected from random sequence space constitute an enormous array of catalytic, diagnostic and therapeutic molecules. Despite the fact that proteins are robust polymers with far greater chemical and physical diversity, success in unlocking protein sequence space remains elusive. We have devised a combinatorial strategy for accessing nucleic acid sequence space corresponding to proteins comprising selected amino acid alphabets. Using the SynthOMIC approach (synthesis of ORFs by multimerizing in-frame codons), representative libraries comprising four amino acid alphabets were fused in-frame to the lambda repressor DNA-binding domain to provide an in vivo selection for self-interacting proteins that re-constitute lambda repressor function. The frequency of self-interactors as a function of amino acid composition ranged over five orders of magnitude, from approximately 6% of clones in a library comprising the amino acid residues LARE to approximately 0.6 in 10(6) in the MASH library. Sequence motifs were evident by inspection in many cases, and individual clones from each library presented substantial sequence identity with translated proteins by BLAST analysis. We posit that the SynthOMIC approach represents a powerful strategy for creating combinatorial libraries of open reading frames that distils protein sequence space on the basis of three inherent properties: it supports the use of selected amino acid alphabets, eliminates redundant sequences and locally constrains amino acids.


Subject(s)
Bacteriophage lambda/genetics , Codon/genetics , DNA-Binding Proteins/genetics , DNA/metabolism , Open Reading Frames/genetics , Peptide Library , Protein Biosynthesis/genetics , Repressor Proteins/genetics , Viral Regulatory and Accessory Proteins/genetics , Amino Acid Sequence , DNA-Binding Proteins/metabolism , Molecular Sequence Data , Repressor Proteins/metabolism , Sequence Homology, Amino Acid , Viral Regulatory and Accessory Proteins/metabolism
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