[Control of plasma aluminium in hemodialyzed patients. Optimization of the method]. / Controllo dell'alluminio plasmatico nei pazienti in emodialisi. Ottimizzazione del metodo.

As concerning biological matrix, the analytic technique in AA-ETA if on one hand shows remarkable sensitivity, on the other it offers reasons that make it not much reproducible. Our aim has been to optimize and standardize the methodic for the dosage of the Al-s paying a special attention on the contamination risks and on the interference problems in reading phase. If we choose accurately consumption materials and the reagents, aluminium contamination problems may be remarkably reduced avoiding all those decontamination operations often complicated; besides it is possible optimize a thermical program which allows the dosage of the Al-s without using chemical modificator. In this work has been practiced a quality control on 103 couples of samples relative to 62 dialyzed patients and blinded compared to the operator, obtaining a CV of 5.4% on the total average of 45.2 micrograms/l. We are convinced that at the present state the high quality of reagents and the utilisation of disposable materials individually wrapped, and even less the remarkable progress of electronics instrumentation of the last generation allow the use of this technique in a widespread way also in laboratories not particularly specialized.

Primary prevention: HBV vaccination in hemodialysis unit.

The authors evaluated the response rate to HBV vaccination in 27 dialyzed patients. In the 1st stage, the response was 70.37%; nonresponder patients (n = 8) were treated with thymostimulin and revaccinated. The total percentage increased to 85.18%. In order to restore a normal response, the authors suggest to treat nonresponder patients with thymostimulin before revaccination.