ABSTRACT
A previously developed high performance chromatographic method has been modified slightly and used to determine the forms of vitamin B6 in human milk. The chromatographic traces are free of compounds that would interfere with the two principal forms found, pyridoxal and pyridoxal phosphate. The method is fast and reliable; it yields recoveries of from 90 to 106% for the vitamers and agrees with results obtained on the same samples with the standard microbiological assay.
Subject(s)
Milk, Human/analysis , Pyridoxine/isolation & purification , Biological Assay , Chromatography, High Pressure Liquid , Female , Humans , Pyridoxine/classificationABSTRACT
An extraction procedure that can be used successfully with a high performance liquid chromatography sample clean-up and analytical method is outlined for the determination of B6 vitamer and pyridoxic acid concentrations in human plasma. The method is a modification of ones developed for food and animal tissue investigations. Chromatographic traces, relatively clean of interfering compounds are obtained. The method has the advantage of speed and reproducibility and yields recoveries of from 90 to 100% for all vitamers and pyridoxic acid.
