Subject(s)
Acetylcysteine/administration & dosage , Lung/physiopathology , Pulmonary Emphysema/physiopathology , Sodium Chloride/administration & dosage , Acetylcysteine/pharmacology , Airway Resistance/drug effects , Cadaver , Humans , Pressure , Sodium Chloride/pharmacology , Spirometry , Therapeutic Irrigation , Thorax , Vital CapacitySubject(s)
Airway Resistance , Pulmonary Emphysema/physiopathology , Pulmonary Ventilation , Adult , Age Factors , Aged , Autopsy , Bronchi/physiopathology , Catheterization , Female , Humans , Male , Middle Aged , Pressure , Vital CapacitySubject(s)
Airway Resistance , Pulmonary Emphysema/physiopathology , Autopsy , Bronchitis/diagnostic imaging , Bronchitis/pathology , Bronchitis/physiopathology , Bronchography , Catheterization , Female , Humans , In Vitro Techniques , Male , Methods , Motion Pictures , Pulmonary Emphysema/diagnostic imaging , Pulmonary Emphysema/pathology , SpirometryABSTRACT
In a 4-year period, 196 lungs from patients with and without chronic obstructive pulmonary disease were examined postmortem for the presence of atrophy in segmental and subsegmental bronchi. As a result of simultaneous postmortem spirometry, cinefluorobronchography and partitioning of airways resistance, plus later assessment of anatomic emphysema, bronchial atrophy emerges as only one of at least three factors usually cooperating in production of abnormal expiratory airway collapse. In selected cases, bronchial atrophy appears to be an important contributor to expiratory airways obstruction.
Subject(s)
Bronchi/pathology , Bronchial Diseases/pathology , Lung Diseases, Obstructive/pathology , Adolescent , Adult , Aged , Airway Obstruction/etiology , Atrophy , Autopsy , Bronchial Diseases/complications , Bronchography , Chronic Disease , Cineradiography , Humans , Middle Aged , Pulmonary Emphysema/pathology , SpirometrySubject(s)
Airway Obstruction/etiology , Bronchial Diseases/complications , Electrocardiography , Pulmonary Emphysema/complications , Aged , Bronchial Diseases/physiopathology , Cardiomegaly/diagnosis , Cardiomegaly/pathology , Diagnosis, Differential , Female , Heart Ventricles , Humans , Male , Middle Aged , Myocardium/pathology , Pulmonary Emphysema/physiopathologySubject(s)
Cell Nucleolus/metabolism , Dactinomycin/pharmacology , HeLa Cells/metabolism , Neoplasm Proteins/metabolism , Amino Acids/metabolism , Binding Sites , Cytoplasm , Electrophoresis, Disc , HeLa Cells/drug effects , Humans , Methionine/metabolism , Ribosomes/metabolism , Time Factors , Tritium , Uridine/metabolismSubject(s)
Cell Nucleolus , Fluorescent Antibody Technique , Staining and Labeling , Acetone/pharmacology , Animals , Blood Proteins/analysis , Carcinoma , Cell Line , Culture Techniques , Fluoresceins , Haplorhini , HeLa Cells/analysis , Histocytochemistry , Hot Temperature , Humans , Hydrogen-Ion Concentration , Kidney , Laryngeal Neoplasms , Metals , Neoplasm Proteins/analysis , Protein Binding , Serum Globulins/metabolism , ThiocyanatesSubject(s)
Pulmonary Emphysema/diagnosis , Aged , Autopsy , Diagnosis, Differential , Female , Humans , Male , Middle Aged , Pulmonary Emphysema/epidemiology , Pulmonary Emphysema/pathologySubject(s)
Pulmonary Emphysema/epidemiology , Adult , Age Factors , Aged , Bronchitis/mortality , Colorado , Humans , Lung/pathology , Male , Middle Aged , Pulmonary Emphysema/mortality , Pulmonary Emphysema/pathologySubject(s)
Bronchi/pathology , Respiratory Tract Diseases/pathology , Aged , Atrophy , Bronchitis/pathology , Bronchography , Cineradiography , Female , Humans , Male , Middle Aged , Pulmonary Emphysema/pathology , RespirationSubject(s)
Autopsy/standards , Bronchitis/mortality , Death Certificates , Pulmonary Emphysema/mortality , Adult , Aged , Chronic Disease , Colorado , Female , Humans , Lung/pathology , Male , Middle Aged , Pulmonary Emphysema/pathologySubject(s)
Anemia, Hemolytic/complications , Mycoplasma Infections/complications , Mycoplasma/isolation & purification , Thromboembolism/complications , Adrenocorticotropic Hormone/therapeutic use , Aged , Antibiosis , Dexamethasone/therapeutic use , Female , Humans , Mycoplasma Infections/drug therapy , Mycoplasma Infections/pathology , Pneumonia/microbiology , Serum Albumin/therapeutic use , Tetracycline/therapeutic useABSTRACT
Nucleoli of animal cells cultured in vitro are modified by a component of "nonimmune" animal serum. Modified nucleoli bind fluorescein-conjugated nonimmune serum proteins, as shown by calcium ion-dependent fluorescence. Analysis of serum indicates that the nucleolar-binding component is a globulin, with an electrophoretic mobility in the same region as the slow alpha-1 component in pH 8.6 Veronal buffer. The component has a low sedimentation constant (2.4S), and appears to contain glycoprotein with relatively high sialic acid content (8.5%); the latter moiety may be essential to reaction with nucleoli. The nucleolar component reacting with this alpha globulin fraction appears to be a histonelike basic protein. Primary cultures of animal cells have been supported for 1 wk through attachment, spreading, and outgrowth from colonies to confluent monolayers in medium containing a nucleolar-reactive serum fraction as the only protein supplement.