ABSTRACT
The aim of current study was to optimize the extraction process and purification of main components (MC) to obtain high antioxidant, anti-inflammatory effective fractions from crabapple (Malus prunifolia (Willd.) Borkh.). The effects of three variables including ethanol concentration A1, solid-liquid ratio A2, extraction temperature A3 were investigated and optimized by response surface methodology (RSM) coupled with Box-Behnken design (BBD). The adsorption/desorption characteristics of MC on the five types of macroporous resins were investigated. According to batch adsorption test, HPD-300 resins were selected for kinetics. The adsorption mechanism showed that the process was appropriate by pseudo-second-order kinetics model, and purification parameters of MC were optimized through adsorption/desorption experiments with the column packed by HPD-300 resin. The effective fractions were obviously superior to other fractions according to DPPH, ABTS, COX-2 and 15-LOX radical scavenging. This work implies that the purified active fraction with high contents of antioxidants and anti-inflammatory compounds from crabapple might be potential source for natural products and food industries.
Subject(s)
Antioxidants , Malus , Antioxidants/pharmacology , Malus/chemistry , Ethanol , Plant Extracts/chemistry , Temperature , Resins, Plant/chemistryABSTRACT
Objective To establish a method for quality evaluation of Nardostachyos Radix et Rhizoma derived from Nardostachys jatamansi DC by determining the contents of nardosinone and establishing fingerprints. Methods HPLC fingerprint and content determination methods were established to evaluate ten batches of Nardostachyos Radix et Rhizoma from Nardostachys jatamansi DC.For the determination of nardosinone, the samples were separated by Phenomenex-C18 ( 4. 6 mm × 250 mm, 5 μm) with the mobile phase consisting of acetonitrile and water at the flow rate of 0. 8 mL · min-1 , the detection wavelength was set at 250 nm,and column temperature was 25℃.The gradient mobile phase system was used for the fingerprints. Results HPLC fingerprint of Nardostachyos Radix et Rhizoma was established with good chromatography separation and repeatability, which could be used for quality control of Nardostachyos Radix et Rhizoma. After similarity analysis, the results showed that there was no significantly difference between the HPLC fingerprints of samples from different origins,but the content of four major peaks were different.The amount of nardosinone from ten batches of Nardostachyos Radix et Rhizoma was determined by HPLC-DAD, which showed that the contents of nardosinone from Nardostachys jatamansi DC were obviously different with each other. Conclusion The method is sensitive, repeatable and accurate. It can be used as a method of quality control for Nardostachyos Radix et Rhizoma.
ABSTRACT
Objective To establish a method for quality evaluation of Nardostachyos Radix et Rhizoma derived from Nardostachys jatamansi DC by determining the contents of nardosinone and establishing fingerprints. Methods HPLC fingerprint and content determination methods were established to evaluate ten batches of Nardostachyos Radix et Rhizoma from Nardostachys jatamansi DC.For the determination of nardosinone, the samples were separated by Phenomenex-C18 ( 4. 6 mm × 250 mm, 5 μm) with the mobile phase consisting of acetonitrile and water at the flow rate of 0. 8 mL · min-1 , the detection wavelength was set at 250 nm,and column temperature was 25℃.The gradient mobile phase system was used for the fingerprints. Results HPLC fingerprint of Nardostachyos Radix et Rhizoma was established with good chromatography separation and repeatability, which could be used for quality control of Nardostachyos Radix et Rhizoma. After similarity analysis, the results showed that there was no significantly difference between the HPLC fingerprints of samples from different origins,but the content of four major peaks were different.The amount of nardosinone from ten batches of Nardostachyos Radix et Rhizoma was determined by HPLC-DAD, which showed that the contents of nardosinone from Nardostachys jatamansi DC were obviously different with each other. Conclusion The method is sensitive, repeatable and accurate. It can be used as a method of quality control for Nardostachyos Radix et Rhizoma.
