Periodontal Diseases in Adult and Elderly Nigerians: A National Survey.

BACKGROUND: Periodontitis has been shown to have links with general health and increase the risk of complications of some systemic diseases now showing a rising prevalence with age. The rising proportion of the elderly globally, and a high prevalence of periodontal disease among older adults may significantly impact the need for oral health care services in the near future. This national survey was carried out to highlight the trend of periodontal diseases among adult and elderly Nigerians and make evidence-based recommendations for good management outcomes. MATERIALS AND METHODS: A national cross-sectional survey of periodontal diseases (using the CPITN Index) among adult (35-44-year-old) and elderly (65-74-year-old) Nigerians selected by multistage sampling method. RESULTS: Periodontal disease is prevalent among adults (96.5%) and elderly (97.6%) Nigerians, with the zonal prevalence approximating the national prevalence. Gingivitis is the prevalent periodontal disease type in these age groups, with a national prevalence of 86.8%, 67.6% respectively, and more in the South than the North for both adults (90.7%, 83%) and elderly (75.7%, 59.7%), respectively (p<0.005). Destructive Periodontitis prevalence in adult and elderly Nigerians is 9.5%, 29.9%, respectively (p<0.05), with the North being more affected than South for both adults (13.4%, 5.6%) and elderly (37%, 22.7%), respectively (p<0.05). There is also a significant rise in the occurrence of destructive periodontitis with age, nationally and in all the zones (p<0.05). CONCLUSION: There is a high prevalence of periodontal diseases in adult and elderly Nigerians. The outcome of the management of periodontal diseases in adults and the elderly is inter-dependent on best practices in both oral and general health care service provision.

CONTEXTE: La parodontite a été liée à la santé générale et à l'augmentation du risque de complications de certaines maladies systémiques, montrant maintenant une prévalence croissante avec l'âge. La proportion croissante de personnes âgées dans le monde et une prévalence élevée des maladies parodontales chez les personnes âgées pourraient avoir un impact significatif sur le besoin de services de santé bucco-dentaire dans un avenir proche. Cette enquête nationale a été réalisée pour mettre en évidence la tendance des maladies parodontales chez les adultes et les personnes âgées nigérianes et formuler des recommandations fondées sur des preuves pour de bons résultats en matière de gestion. MATÉRIEL ET MÉTHODES: Une enquête nationale transversale sur les maladies parodontales (utilisant l'indice CPITN) parmi les adultes (35-44 ans) et les personnes âgées (65-74 ans) nigérianes sélectionnées par méthode d'échantillonnage à plusieurs niveaux. RÉSULTATS: Les maladies parodontales sont prévalentes chez les adultes (96,5 %) et les personnes âgées (97,6 %) nigérianes, la prévalence zonale approchant la prévalence nationale. La gingivite est le type de maladie parodontale prédominant dans ces groupes d'âge, avec une prévalence nationale de 86,8 %, 67,6 % respectivement, et plus dans le Sud que dans le Nord pour les adultes (90,7 %, 83 %) et les personnes âgées (75,7 %, 59,7 %), respectivement (p<0,005). La prévalence de la parodontite destructrice chez les adultes et les personnes âgées nigérianes est de 9,5 %, 29,9 %, respectivement (p<0,05), le Nord étant plus touché que le Sud pour les adultes (13,4 %, 5,6 %) et les personnes âgées (37 %, 22,7 %), respectivement (p<0,05). On observe également une augmentation significative de l'occurrence de la parodontite destructive avec l'âge, nationalement et dans toutes les zones (p<0,05). CONCLUSION: Il existe une prévalence élevée des maladies parodontales chez les adultes et les personnes âgées nigérianes. Les résultats de la gestion des maladies parodontales chez les adultes et les personnes âgées dépendent des meilleures pratiques dans la prestation de services de santé bucco-dentaire et générale. MOTS CLÉS: Prévalence, Gingivite, Parodontite, Nationale, Enquête.

Isolation and Identification of Phytocompounds from Maytenus dhofarensis and Their Biological Potentials.

Maytenus dhofarensis Sebsebe (Celestraceae) is a naturally growing shrub in Oman. It is not a reputed medicinal plant in Oman, but it is regionally endemic and causes shivering attacks on goats that graze on it. The chemical investigation of the hexane and chloroform extracts of the fruits and stems of M. dhofarensis afforded dihydro-ß-agarofuran-type sesquiterpene pyridine alkaloid (1), lupanyl myristoate (2) and lignanolactone (3). Compounds (1-3) are new isolates from M. dhofarensis. The structures of these compounds were assigned through comprehensive IR, NMR, and ESI-MS analyses, and the relative configurations of compounds 1 and 3 were deduced from density function theory (DFT) calculations and NMR experiments. Compound 1 was assayed against the kinase enzyme and showed no inhibition activity for p38 alpha and delta at a 10 µM test concentration. Compound 3 inhibited the 2,2'-diphenyl-1-picrylhydrazyl radical (DPPH) by 69.5%, compared to 70.9% and 78.0% for gallic acid and butylated hydroxyanisole, respectively, which were used as positive controls.

Prevalence of Complete Edentulism in Adults and Older Nigerian Population.

INTRODUCTION: The prevalence of complete edentulism varies from country to country and from one region to another region, and making comparisons between national data challenging because of the impact of lifestyle, socio-economic and educational factors. There is no reported national data for Nigeria. AIMS AND OBJECTIVES: The study was to determine the prevalence of complete edentulism among adult (35-44 years) and older (65-74 years) Nigerians, being part of a national oral health survey of Nigerians conducted in 2014. METHODOLOGY: The study was a cross-sectional survey of oral diseases, specifically dental caries, periodontal diseases, dental trauma, and tooth loss, among adult and older Nigerian populations using a multi-stage stratified sampling technique for all 36 states and the FCT, and clinical examination in field situations using daylight settings, with findings collected using data entry forms (WHO 1997). Ethical approvals were obtained from the FMOH, SMOH and SMLGCA. Data was analysed using SPSS-11 and nominal variables compared using chi-square. A p-value of 0.05 was considered significant. RESULTS: A total of 5,038 adults and 4,658 older Nigerians were examined, of which 4(0.08%) and 84 (1.8%), respectively had complete edentulism. Among the adults, it was reported exclusively in Northern Nigeria; whereas among the older population, 66(2.8%) and 18(0.8%) participants in Northern and Southern Nigeria respectively, were reported to be edentulous (p<0.05). 45 cases (4.7%) of edentulism in older Nigerians were reported from the North West geopolitical zone alone. Edentulism is higher in the older population than the adult population in both Northern and Southern regions (p<0.05). Socio-demographic variation was significant in the North, with a higher prevalence in the rural localities among the older population (p<0.05). CONCLUSION: The study showed that there are variations in the prevalence of edentulism in Nigeria, reflecting lower prevalence in comparison with global trends. National prevalence values did not reflect variations along geographical and socio-political divide.

INTRODUCTION: La prévalence de l'édentation complète varie d'un pays à l'autre et d'une région à l'autre, ce qui rend les comparaisons entre les données nationales difficiles en raison de l'impact des facteurs liés au mode de vie, à la situation socio- économique et à l'éducation. Il n'existe pas de données nationales pour le Nigéria. BUTS ET OBJECTIFS: L'étude visait à déterminer la prévalence de l'édentation complète chez les Nigérians adultes (35-44 ans) et plus âgés (65-74 ans), dans le cadre d'une enquête nationale sur la santé bucco-dentaire des Nigérians menée en 2014. MÉTHODOLOGIES: L'étude était une enquête transversale sur les maladies bucco-dentaires, en particulier les caries dentaires, les maladies parodontales, les traumatismes dentaires et la perte de dents, parmi les populations nigérianes adultes et âgées, en utilisant une technique d'échantillonnage stratifiée à plusieurs niveaux pour les 36 États et le FCT, et un examen clinique dans des situations de terrain utilisant des environnements de lumière du jour, avec des résultats recueillis à l'aide de formulaires de saisie de données (OMS 1997). Les autorisations éthiques ont été obtenues auprès de la FMOH, de la SMOH et de la SMLGCA. Les données ont été analysées à l'aide du logiciel SPSS -11 et les variables nominales ont été comparées à l'aide du chi-carré. Une valeur p de 0,05 a été considérée comme significative. RÉSULTATS: Au total, 5 038 adultes et 4 658 Nigérians âgés ont été examinés, dont 4 (0,08 %) et 84 (1,8 %), respectivement, souffraient d'édentation complète. Chez les adultes, l'édentation a été signalée exclusivement dans le nord du Nigeria, tandis que chez les personnes âgées, 66 (2,8 %) et 18 (0,8 %) participants du nord et du sud du Nigeria respectivement ont été déclarés édentés (p<0,05). 45 cas (4,7%) d'édentulisme chez des Nigérians âgés ont été signalés dans la seule zone géopolitique du Nord-Ouest. L'édentulisme est plus élevé dans la population âgée que dans la population adulte, tant dans les régions du Nord que du Sud (p<0,05). Les variations sociodémographiques étaient significatives dans le Nord, avec une prévalence plus élevée dans les localités rurales parmi la population âgée (p<0,05). CONCLUSION: L'étude a montré qu'il existe des variations dans la prévalence de l'édentation au Nigéria, reflétant une prévalence plus faible par rapport aux tendances mondiales. Les valeurs de prévalence nationale ne reflètent pas les variations géographiques et sociopolitiques. Mots-clés: Édentulisme, Prévalence, Adultes, Personnes âgées, Nigérians.

Visual Morbidities among Patients at a Geriatric Center in Southwest Nigeria and the Merits of Designated Geriatric Health Care Centers Incorporating Eye Care Services.

BACKGROUND: : Visual morbidities increase the burden of care and negatively impact the quality of life of older people. Few empirical reports exist on the visual status of older Nigerians. AIM: This study describes the visual morbidities and determinants of visual impairment among persons aged 60 years and above who presented at a geriatric center in southwestern Nigeria and discusses the merits of focused geriatric care at a single location. MATERIALS AND METHODS: Data were obtained from electronic health records and case files of 628 older patients (≥60 years) who attended the facility between January 1, 2014 and December 31, 2018. Data extracted included, age, sex, married status, occupational status, visual acuity, eye diagnoses, eye medications, previous surgeries, and anthropometric measurements. RESULTS: Mean age of patients was 70.1 ± 7.4 years and 378 (60.2%) were females. Two out of every three patients had more than one visual diagnosis. Significantly, glaucoma (P < 0.001) and cataract (P = 0.01) were common among men, whereas dry eye syndrome (P < 0.001) and allergic conjunctivitis (P = 0.01) were common in women. Antiglaucoma medications (55.8%) were the commonest medications used and 21.0% had previous eye surgery. Assessment of presenting visual acuity demonstrated that 28.7% of patients had moderate-severe visual impairment (MSVI) in both eyes at presentation while 10.3% were bilaterally blind. Increasing age (P < 0.001) and male sex (P = 0.01) were the factors significantly associated with blindness. CONCLUSION: MSVI and blindness were common in our setting with glaucoma and cataract being the most prevalent associated diagnosis. Information obtained should stimulate advocacy for the prompt management of preventable causes of poor vision in older Nigerians.

Dental Trauma in Adult and Elderly Nigerians: A National Survey.

BACKGROUND: Various studies on Traumatic Dental Injuries (TDI) have been carried out in different small localities and mostly among children and adolescents in Nigeria, but there has not been any national study to determine its prevalence. This national survey was carried out to understand the complexities of dental trauma epidemiology among adult and elderly Nigerians. MATERIAL AND METHODS: A national cross-sectional survey of TDI among adult and elderly Nigerians, following WHO recommendations. RESULTS: A total of 5,067 adults (35-44 years) and 4,680 elderly (65-74 years) Nigerians were examined, and the prevalence of dental trauma among the adult and elderly was 15.9 % and 22.1%, respectively. Gender comparison showed males were more affected than females in the North (p<0.05), while TDIs occurred more among females in Southern Nigeria (p<0.05). In Nigeria, Type 2 injuries were most prevalent among the adults and elderly (53.9% and 55.7%, respectively). Among the elderly, while Type 1 injuries were more common in the North, Type 2 injuries were more prevalent in Southern Nigeria (p<0.05). Types 3-10 injuries were more prevalent in the South (p<0.05). TDI 1,2 occurred more in the anterior teeth among adults (p<0.05) and in posterior teeth among the elderly, especially in Southern Nigeria (p<0.05); while TDI 3-10 affected mostly the anterior teeth in both age groups (p<0.05. CONCLUSIONS: The observed pattern of dental trauma among the adults and elderly Nigerians could reflect the diverse dietary and socio-cultural practices. Advocacy for prevention of, relevant education on, research into and improvement of access to treatments for dental trauma is recommended.

CONTEXTE: Diverses études sur les traumatismes dentaires (TDI) ont été menées dans différentes petites localités et principalement chez des enfants et des adolescents au Nigéria, mais aucune étude nationale n'a été menée pour déterminer sa prévalence. Cette enquête nationale a été menée pour comprendre les complexités de l'épidémiologie des traumatismes dentaires chez les Nigérians adultes et âgés. MATÉRIEL ET MÉTHODES: Une enquête nationale transversale sur le TDI parmi les Nigérians adultes et âgés, conformément aux recommandations de l'OMS. RÉSULTATS: Un total de 5 067 adultes (35 à 44 ans) et 4 680 personnes âgées (65 à 74 ans) Nigérians ont été examinés, et la prévalence des traumatismes dentaires chez les adultes et les personnes âgées était de 15,9% et 22,1%, respectivement. La comparaison entre les sexes a montré que les hommes étaient plus touchés que les femmes dans le Nord (p <0,05), tandis que les IDT se produisaient davantage chez les femmes du sud du Nigeria (p <0,05). Au Nigéria, les blessures de type 2 étaient les plus fréquentes chez les adultes et les personnes âgées (53,9% et 55,7%, respectivement). Chez les personnes âgées, alors que les blessures de type 1 étaient plus fréquentes dans le nord, les blessures de type 2 étaient plus fréquentes dans le sud du Nigéria (p <0,05). Les blessures de type 3 à 10 étaient plus fréquentes dans le Sud (p <0,05). Le TDI 1,2 s'est produit davantage dans les dents antérieures chez les adultes (p <0,05) et dans les dents postérieures chez les personnes âgées, en particulier dans le sud du Nigéria (p <0,05); tandis que le TDI 3-10 affectait principalement les dents antérieures dans les deux groupes d'âge (p <0,05). CONCLUSIONS: Le modèle observé de traumatisme dentaire chez les adultes et les Nigérians âgés pourrait refléter les diverses pratiques alimentaires et socioculturelles. Il est recommandé de plaider en faveur de la prévention, de l'éducation pertinente, de la recherche et de l'amélioration de l'accès aux traitements des traumatismes dentaires. MOTS CLÉS: Blessures dentaires traumatiques, types, dentaires, traumatismes, adultes, personnes âgées, Nigéria.

Macular diseases in Ibadan, Nigeria.

BACKGROUND: Retina diseases including the diseases of the macular are underreported in developing countries of sub-Sahara Africa including Nigeria. METHOD: A retrospective review of retinal register of cases presenting to the retinal clinic of the University College Hospital, Ibadan within 4 years (December 2015, to November 2019). Demographics and clinical data of all patients with macula diseases were retrieved. Data were analysed using the Statistical Package for Social Sciences IBM (SPSS-IBM), version 22 (SPSS Inc., Chicago, Illinois, USA), and reported as frequency distributions and percentages. RESULTS: A total of 1291 retinal cases were seen during the period under review, out of which 322 cases were diseases of the macula, representing 24.9% of retinal cases seen. The top 3 common causes of macular disease found in the study were dry Age-related Macular Degeneration (AMD) 63 (19.6%); Macula oedema 53 (16.5%) and Non-AMD atrophic maculopathy (from Retinitis Pigmentosa, chloroquine maculopathy and hereditary causes) 51 (15.9%) representing more than 50% of cases. Macular holes 48 (14.9%); Non-AMD macular scar (Toxoplasmosis, Trauma) 37 (11.5%) and choroidal neovascular membrane (CNVM) 26 (8.1%) are other important causes. Idiopathic Polypoidal Choroidal Vasculopathy (IPCV) 17 (5.3%) is an emerging cause of macular disease in the retina unit of the University College Hospital Ibadan. CONCLUSION: Age-related macular degeneration (AMD), Macular oedema and Non-AMD atrophic maculopathy are major causes of macular disease presentation in the retinal clinic of the University College Hospital Ibadan, Nigeria. CNVM and IPCV are emerging causes.

Forensic mental health service implications of COVID-19 infection in Nigeria.

Forensic mental health services are fledgling in most African countries including Nigeria. The current formulation of such services in Nigeria is correctional psychiatry in addition to some hospital-based services. The COVID-19 pandemic presents significant challenges to the already fragile state of forensic mental health services within the country. Its impact includes limitation of access to and continuity of mental healthcare in prison as well as reduction in referrals from other services within the criminal justice system such as the police and the courts. Further tapering of previously less prominent aspects of forensic mental health service in the country such as the preparation of psychiatric court reports has also been observed. Forensic psychiatry training and research have equally been hampered by the pandemic. The lessons learnt from these challenges should offer practitioners and policy makers insight into strategic developmental objectives for the post-COVID era within services and training programmes.

Vision-related quality of life assessment of patients attending a geriatric centre in south-west Nigeria

According to World Health Organization, health not only refers to the absence of physical diseases, but also includes psychological and social well-being of individuals.[1] Quality of life (QoL) on the other hand, lacks a single, universally accepted definition.[2] However, QoL may be defined subjectively as "an individual's perception of his/her life in the context of the culture and value system in which they live, and in relation to their goals, expectations, standards and concerns.[3] QoL also has an objective component which can be measured objectively using validated instruments (e.g. questionnaires) in which a score is assigned to specific indices or characteristics in different domains along a scale in a continuum.[4],[5],[6]Visual function is important for optimal orientation in functional and social life and has effects on physical, psychological, mental and emotional well-being of the individual.[7] Visual function therefore is a domain under which QoL may be assessed. This is referred to as vision-specific or vision-related QoL. The National Eye Institute Visual Function Questionnaire (NEI-VFQ) is an example of an instrument designed and validated for the objective assessment of vision-specific QoL.[8]Broman et al.[9] revealed that a visual acuity of at least 6/12 was found to negatively impact on n individual's quality of life. This was also reiterated by Bekibele et al.[10] The Proyecto VER study[9] observed that visual impairment was associated with a decrease in the quality of life among elderly patients, and that the severity of ocular diseases was related to the level of visual impairment. In relation to persons with no visual impairment, persons with bilateral mild and unilateral or bilateral moderate or severe visual impairment report greater difficulties in performing most vision-dependent daily activities, experience vision-related dependency and poorer vision-related mental health.[11]Ee Munn Chia et al.[12] further disclosed that the impact of visual impairment on mental domains was much greater compared to co-existing medical conditions like stroke.Therefore, data is needed on the effect of ocular diseases on vision-related quality of life among the elderly for the purpose of making recommendations for improved eye care with resultant improvement in their quality of life

UTILIZATION OF EYE CARE SERVICES AMONG STUDENTS ATTENDING SCHOOLS FOR THE HEARING IMPAIRED IN OYO STATE, SOUTH-WEST NIGERIA.

BACKGROUND: Vision screening and detection of ophthalmic disorders in hearingimpaired individuals is important to optimise their visual function and therefore, their quality of life. OBJECTIVE: To determine the utilisation of eye care services among students attending schools for the hearing impaired in Oyo State, Nigeria. METHODS: This was a descriptive cross-sectional study conducted among hearingimpaired students aged 11 years and above. Using systematic random sampling, participants were selected from four schools. An interviewer administered semistructured questionnaire was used to collect socio-demographic data and information on history of eye symptoms and utilisation of eye care services. Data analysis was performed using the IBM SPSS software version 22 and test of association done using chi-square test with level of statistical significance set at p-value <0.05. RESULTS: A total of 335 students were recruited into the study. Mean age was 17 ± 2.9 years (range 11 - 39 years). Only 147 (43.9%) respondents had utilised eye care services in the past. Some of the reasons given for non-utilisation include financial constraints and lack of an escort to the hospital. CONCLUSION: This study has demonstrated that the rate of utilisation of eye care services among the studied population is suboptimal. Therefore, it is essential for government, eye care providers and other stakeholders to design and implement policies and eye care programmes that would improve the uptake of eye care services among hearing-impaired individuals. This will reduce the compounding effect of visual impairment in such individuals.

Pomegranate seed oil: Effect on 3-nitropropionic acid-induced neurotoxicity in PC12 cells and elucidation of unsaturated fatty acids composition.

BACKGROUND: Seed oils are used as cosmetics or topical treatment for wounds, allergy, dandruff, and other purposes. Natural antioxidants from plants were recently reported to delay the onset or progress of various neurodegenerative conditions. Over one thousand cultivars of Punica granatum (Punicaceae) are known and some are traditionally used to treat various ailments. AIM: The effect of pomegranate oil on 3-nitropropionic acid- (3-NP) induced cytotoxicity in rat pheochromocytoma (PC12) neuronal cells was analyzed in this study. Furthermore, the analysis of unsaturated fatty acid composition of the seed oil of pomegranate by gas chromatography-electron impact mass spectrometry (GC-MS) was done. RESULTS: GC-MS study showed the presence of 6,9-octadecadiynoic acid (C18:2(6,9)) as a major component (60%) as 4,4-dimethyloxazoline derivative. The total extractable oil with light petroleum ether by Soxhlet from the dry seed of P. granatum was 4-6%. The oil analyzed for 48.90 ±â€Š1.50 mg gallic acid equivalents/g of oil, and demonstrated radical-scavenging-linked antioxidant activities in various in vitro assays like the DPPH (2,2-diphenyl-l-picrylhydrazyl, % IP = 35.2 ± 0.9%), ABTS (2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid), % IP 2.2 ± 0.1%), and ß-carotene bleaching assay (% IP = 26 ± 3%), respectively, which could be due the possible role of one methylene interrupted diynoic acid system for its radical-scavenging/antioxidant properties of oil. The oil also reduced lipid peroxidation, suppressed reactive oxygen species, extracellular nitric oxide, lactate/pyruvate ratio, and lactase dehydrogenase generated by 3-NP- (100 mM) induced neurotoxicity in PC12 cells, and enhanced the levels of enzymatic and non-enzymatic antioxidants at 40 µg of gallic acid equivalents. CONCLUSION: The protective effect of pomegranate seed oil might be due to the ability of an oil to neutralize ROS or enhance the expression of antioxidant gene and the exact mechanism of action yet to be elucidated.

ent-Kaurene diterpenoids from Blepharispermum hirtum.

The twigs and leaves of Blepharispermum hirtum Oliver (Asteraceae) were investigated for their larvicidal and antimicrobial activity. Fractionation of the extracts of the twigs, directed by brine shrimp test and antibacterial activities, led to the isolation of compounds 1-4; two of which are new ent-kaurene diterpenoids, blepharispins A and B (1 and 2, resp.). The structures of compounds 1 and 2 were established from spectral data. The absolute configuration at C(15) in 1 was inferred from Mosher ester analysis and relative configurations were suggested by a NOESY experiment. Compound 4 was significantly larvicidal to newly hatched naupleii of Artemia salina L. (BST LC(50)=1.3 (3.7-0.0) microg/ml), but the blepharispins were not (BST LC(50) > 500 microg/ml). Nevertheless, compound 1 inhibited the growth of Staphylococcus aureus and Bacillus subtilis at a MIC value of 62.5 microg/ml. The significance of the bioactivity results and the presence of ent-kaurene diterpenoids in B. hirtum are discussed from biosynthetic and local utilization viewpoints.

Identification of the chemotypes of Ocimum forskolei and Ocimum basilicum by NMR spectroscopy.

The chemotypes of Ocimum forskolei Benth and Ocimum basilicum L. growing wild in Oman have been established by (13)C-NMR analyses of the vegetative and floral oils of the plants. The chemotypes, estragole for O. forskolei and linalool for O. basilicum, suggested by (13)C-NMR fingerprinting were also confirmed by GC-FID and GC/MS analyses. The oil of O. forskolei demonstrated better activities against bacteria and dermatophytes. The significance of the presence of estragole and linalool in the volatile oils of plants whose fragrances are traditionally inhaled, added to food, or rubbed on the skin are discussed.

Production of phenylacetic acid derivatives and 4-epiradicinol in culture by Curvularia lunata.

Phenylacetic acid derivatives, methyl 2-acetyl-3,5-dihydroxyphenylacetate (1) and methyl 2-acetyl-5-hydroxy-3-methoxyphenylacetate (3); curvulin or ethyl 2-acetyl-3,5-dihydroxyphenylacetate (4), a known metabolite of Curvularia siddiqui, and 4-epiradicinol (5) have been isolated from the culture mycelia of Curvularia lunata grown on YMG, a medium consisting of yeast, malt extract and glucose. Compounds 1, 3 and 4 lack antimicrobial and antioxidant activity, but 4-epiradicinol (5) inhibited the growth of Escherichia coli, Staphylococcus aureus, Salmonella choleraesuis and Bacillus subtilis. The structures of compounds 1, 3-5 were determined by analyses of IR, MS, 1D and 2D NMR data, assisted by chemical shift comparison to related and model compounds. The relative stereochemistry of the vicinal diol in 5 was determined from the 1H NMR signals for the methyl protons of the resulting cyclic acetonide prepared from 5.

Chemical composition, antibacterial and antifungal activities of the essential oil of Haplophyllum tuberculatum from Oman.

The chemical composition of the essential oil of Haplophyllum tuberculatum (Forsskal) A. Juss (Rutaceae) was analyzed by gas chromatography-mass spectral (GC-MS) and 13C NMR spectroscopy. Thirty compounds, constituting about 99.7% of the total oil, were identified. The most abundant oil components are beta-phellandrene (23.3%), limonene (12.6%), (Z)-beta-ocimene (12.3%), beta-caryophyllene (11.6%), myrcene (11.3%), and alpha-phellandrene (10.9%). Ten microlitres (25 mg) of pure oil partially inhibited the growth of Escherichia coli, Salmonella choleraesuis, and Bacillus subtilis to the same extent as 0.10 microg of gentamycin sulfate. The oil also affected the mycelial growth of Curvularia lunata and Fusarium oxysporium in a dose-dependent manner but had no effect on the germination of their spores.

New sesquiterpenes from Pluchea arabica.

Two new sesquiterpenes, godotol A (1) and godotol B (2), were isolated from Pluchea arabica. Their structures were determined by analysis of NMR data. The absolute configurations of 1 and 2 were established by Mosher ester methodology. The godotols displayed weak activity against bacteria and the brine shrimp larvae. They were also inactive in the DPPH antioxidant assay.

Muscanone: a 3-O-(1", 8", 14"-trimethylhexadecanyl)naringenin from Commiphora wightii.

A new antifungal flavanone, muscanone (1), was isolated along with known naringenin (2) from Commiphora wightii (Arn.) Bhandari (Burseraceae) by directing the fractionation of an EtOH extract of the air-dried trunk of C. wightii with microbial sensitivity assay. The structures of 1 and 2 were determined from EIMS, HREIMS, DEPT, 1H-1H COSY, HSQC and HMBC spectral data. Muscanone (1) was identified as 3-O-(1", 8",14"-trimethylhexadecanyl)naringenin and was found to be active against Candida albicans. The isolation, structure elucidation, NMR spectral assignments, and bioactivities of 1 and 2 are reported.

Monoacylglycerol from Punica granatum seed oil.

The seeds of Punica granatum, known as hap roman in the Arabian Peninsula, are commonly eaten as a dessert. As part of an ongoing project to find nonnutritional natural products which have health benefits, or that can be exploited to protect crops, the chloroform-soluble extract of the fermented seeds of P. granatum was found to be rich in 1-O-trans,cis,trans-9,11,13-octadecatrienoyl glycerol (1). The seed oil is not lethal to brine shrimp larvae. 1-O-isopentyl-3-O-octadec-2-enoyl glycerol (2) and the known cis-9-octadecenoic, octadecanoic, and eicosanoic acids were also detected in small amounts in the seed oil by LC and MS. The structure of 1 was determined from NMR and MS spectral data.

Site-directed mutagenesis and X-ray crystallography of the PQQ-containing quinoprotein methanol dehydrogenase and its electron acceptor, cytochrome c(L).

Two proteins specifically involved in methanol oxidation in the methylotrophic bacterium Methylobacterium extorquens have been modified by site-directed mutagenesis. Mutation of the proposed active site base (Asp303) to glutamate in methanol dehydrogenase (MDH) gave an active enzyme (D303E-MDH) with a greatly reduced affinity for substrate and with a lower activation energy. Results of kinetic and deuterium isotope studies showed that the essential mechanism in the mutant protein was unchanged, and that the step requiring activation by ammonia remained rate limiting. No spectrally detectable intermediates could be observed during the reaction. The X-ray structure, determined to 3 A resolution, of D303E-MDH showed that the position and coordination geometry of the Ca2+ ion in the active site was altered; the larger Glu303 side chain was coordinated to the Ca2+ ion and also hydrogen bonded to the O5 atom of pyrroloquinoline quinone (PQQ). The properties and structure of the D303E-MDH are consistent with the previous proposal that the reaction in MDH is initiated by proton abstraction involving Asp303, and that the mechanism involves a direct hydride transfer reaction. Mutation of the two adjacent cysteine residues that make up the novel disulfide ring in the active site of MDH led to an inactive enzyme, confirming the essential role of this remarkable ring structure. Mutations of cytochrome c(L), which is the electron acceptor from MDH was used to identify Met109 as the sixth ligand to the heme.

Bcl10 is involved in t(1;14)(p22;q32) of MALT B cell lymphoma and mutated in multiple tumor types.

MALT B cell lymphomas with t(1;14)(p22;q32) showed a recurrent breakpoint upstream of the promoter of a novel gene, Bcl10. Bcl10 is a cellular homolog of the equine herpesvirus-2 E10 gene: both contain an amino-terminal caspase recruitment domain (CARD) homologous to that found in several apoptotic molecules. Bcl10 and E10 activated NF-kappaB but caused apoptosis of 293 cells. Bcl10 expressed in a MALT lymphoma exhibited a frameshift mutation resulting in truncation distal to the CARD. Truncated Bcl10 activated NF-kappaB but did not induce apoptosis. Wild-type Bcl10 suppressed transformation, whereas mutant forms had lost this activity and displayed gain-of-function transforming activity. Similar mutations were detected in other tumor types, indicating that Bcl10 may be commonly involved in the pathogenesis of human malignancy.