ABSTRACT
The aim of this study was to test whether reperfusion with isotonic standard polyelectrolyte solution alone has any influence on the redox balance in the lung during hemorrhagic shock, and whether reperfusion and BN 52021, used together have better protective effect on free radical lung injury. The results showed that reperfusion with electrolyte solution increased solely glutathione reductase activity (GSSG-R), and diminished the activities of Cu,Zn-superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) as well as the content of SH-groups. Thiobarbituric acid reactive substances (TBA-rs) were much more increased in comparison to hemorrhagic shock. After reperfusion with polyelectrolyte solution and the administration of BN 52021, the antioxidant parameters returned to normal values, while TBA-rs slightly decreased.
Subject(s)
Diterpenes , Free Radical Scavengers/therapeutic use , Lactones/therapeutic use , Lung Diseases/metabolism , Lung/drug effects , Reperfusion Injury/metabolism , Shock, Hemorrhagic/metabolism , Animals , Antioxidants/metabolism , Electrolytes/pharmacology , Female , Ginkgolides , Glutathione Reductase/metabolism , Lung/metabolism , Oxidation-Reduction , Rats , Rats, Wistar , Reperfusion Injury/prevention & control , Superoxide Dismutase/metabolism , Thiobarbituric Acid Reactive Substances/metabolismABSTRACT
The purpose of our experiments was to determine whether BN 52021, PAF-receptor antagonist, has any influence on the production of reactive oxygen species in the lung induced by haemorrhagic shock. The results showed, that the activities of Cu,Zn-superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in the lung after haemorrhagic shock were decreased in comparison with control and sham groups while the activity of glutathione reductase (GSSG-R) was unchanged. The content of SH-groups was decreased while thiobarbituric acid reacting substances (TBA-rs) after haemorrhagic shock were increased. Infusion of BN 52021 (5.0 mg/kg i.v.) in haemorrhagic animals caused significantly increased activities of Cu,Zn-SOD, GSH-Px, GSSG-R in the lung. The SH-group level was slightly increased while TBA-rs returned to normal values.
Subject(s)
Diterpenes , Free Radical Scavengers/pharmacology , Lactones/pharmacology , Lung/drug effects , Platelet Membrane Glycoproteins/antagonists & inhibitors , Reactive Oxygen Species/metabolism , Receptors, Cell Surface , Receptors, G-Protein-Coupled , Shock, Hemorrhagic/metabolism , Animals , Female , Ginkgo biloba/chemistry , Ginkgolides , Glutathione Peroxidase/antagonists & inhibitors , Glutathione Peroxidase/metabolism , Lung/enzymology , Lung/metabolism , Oxidoreductases/antagonists & inhibitors , Oxidoreductases/metabolism , Plant Extracts/pharmacology , Plants, Medicinal , Protective Agents/therapeutic use , Rats , Rats, Wistar , Shock, Hemorrhagic/chemically induced , Shock, Hemorrhagic/enzymologyABSTRACT
OBJECTIVE: Non-enzymatic low-molecular antioxidants are one of the important mechanisms which protect cells against the toxic effect of oxygen. The aim of the present study was to determine the content of glutathione, glutathione reductase, and ascorbic acid in the principal layers of polyester grafts. INTERVENTIONS AND MEASURES: The experiments were carried out on 24 mongrel dogs, in which polyester double velour DALLON grafts were implanted. Seven days, 1, 4, and 12 months after the operation the grafts were excised. The following were determined: glutathione content by use of a GSH-400 system, glutathione reductase activity by the method of Langdon and Mize, and ascorbic acid content by the Kyaw method. RESULTS: It was found that the glutathione content in the graft neointima was 33% lower, in the graft neomedia higher during the first 4 months, in the neoadventitia 50% lower after 4 months than in the corresponding layers of the aorta (p < 0.01). The activity of glutathione reductase was significantly higher in all the graft layers during 12 months' observation than in the normal aorta layers. The ascorbic acid content of the graft layers was lowest 7 days after the implantation, and then in time increased so that 12 months after the operation it reaches its highest values. CONCLUSIONS: Our study shows that low non-enzymatic antioxidative potential is not capable of proteoting the newly forming graft layers, particularly the neointima, against oxygen toxicity. Thus, it would be beneficial to administer antioxidants (vitamin C, vitamin E, and N-acetylcysteine).