[Using of chromatographical methods to study stability, pharmacokinetics and metabolism of propofol (review)].

Propofol (somnopol, diprivan) 2,6-diisoprophilphenol is a preparation of short-term, intravenous narcosis used for local and general anaesthesia through single injection or permanent infusion with the speed of 4-12 mg/kg in an hour. In this study we tried to summarize the experience of using of chromatographical methods (gas chromatography-GCH0, high performance liquid chromatography (HPLC), thin layer chromatography (TLCH) and combination of mass-spectrometry with GCH (GCH-MC) and HPLC (HPL-MC) to study stability, pharmacokinetics and metabolism of propofol. A labour-intensive and important work have been carried out to obtain information about propofol.

[Investigation of conjugation and metabolism of propofol with the method of high performance liquid chromatography].

Propophol (somnopol, diprivan) is widely used in medicine for local and general narcosis through single injection or permanent infusion. Study of conjugation and metabolism of medications is considered as question of great importance, especially for substances used at anaesthesia. To study the above-mentioned questions the total complex of methods of chromatographical analysis, especially, high performance liquid chromatolgraphy (HPLC), are widely used. Objects of investigation were: blood plasma and urine of surgical patients. Method of investigation - HPLC with Ultraviolet-Detector. For investigation of structures of propophol conjugates and its metabolites selective enzymatic and non-selective acid hydrolysis were used. Two metabolites: 2,6-diisoprophil-1,4-bensoxinon and 2,6-diisopropilhydroxinon were determined.

[New methods of chemical and toxicological study of clonidine (Clophelinum)].

Clophelinum (clonidine) has hypotensive and soothing effect. The literature describes cases of a poisoning with clonidine, the majority of which are of the criminal character. Symptoms of clonidine intoxication differ from poisoning with other substances. Clinical picture of poisoning with clonidine is complex enough. Pathological and histological changes in internal organs in case of poisoning with clonidine are non specific. The methods of sampling, isolation, and qualitative determination need improvement. The aim of the research was investigation of chemical and toxicological properties of Clophelinum to develop new techniques of isolation and identification of clonidine. The technique of isolation is characterized by high degree of isolation (96 %), and technique of identification by high sensitivity (0,1 mkg/ml), which proves their efficiecy.

[Clinical significance of genetically determined polymodality of the distribution of quinidine elimination rates]. / Klinicheskoe znachenie geneticheski determinirovannoi polimodal'nosti raspredeleniia skorostei èliminatsii khinidina.

A population study of quinidine half-elimination time distribution has demonstrated a trimodality of distribution that is due to different rates of metabolic quinidine oxidation. The ratio of permanent serum concentrations of 3-oxyquinidine (metabolite) to quinidine in the rapid oxidation group was elevated significantly, as compared to slow oxidation groups. The values obtained in these phenotypic groups show good correspondence to the Hardy-Weinberg law, with a 0.755 frequency of the allele controlling high monooxygenase activity, and a 0.244 frequency of the gene for the low activity. Effective daily doses should be 1.5-2 times as high in high oxidants (57% of the population) as they are in slow oxidants (37%), and 2.5-3 times as high, as compared to very slow oxidants (6%).