Degradation of Nitriles by Mixed Biofilms of Nitrile-Hydrolyzing Bacteria in Submerged Packed-Bed Reactor.

Degradation of nitriles by mixed biofilms of nitrile-hydrolyzing bacteria Alcaligenes faecalis 2 and Rhodococcus ruber gt 1 grown on basalt and carbon carriers, in a submerged packed-bed reactor was studied. It was shown the formation of a massive mixed biofilm of Al. faecalis 2 and R. ruber gt 1 and the effective removal of nitriles and products of their degradation from the reaction medium. After the accumulation of carboxylic acid and some of the unprocessed substrate, the system adapts to 600-1000 h of biofilter operation, which is expressed in a decrease in the content of substrate and reaction products in the medium. The rate of acetonitrile and acrylonitrile utilization was 0.072-0.086 and 0.039-0.215 g/h, respectively, and acrylonitrile utilization with maximum rate was realized by a mixed biofilm on carbon fibers. Biofilms grown on mixed fibers in a "sandwich"-type reactor had the best characteristics for the transformation of aceto- and acrylonitrile (removal capacity of 99.6-99.9%, nitrile utilization rate of 0.080-0.095 g/h). Biofilms grown on basalt fiber with a diameter of 4-12 µm are also well suited for the degradation of acetonitrile (removal capacity of 100%, nitrile utilization rate of 0.086 g/h). The results of metagenomic analysis showed the resistance of Al. faecalis 2 and R. ruber gt 1 mixed biofilms against leaching from a biofilter and to competitive growth in an open system, indicating the advantages of biofilms over homogeneous biomass for wastewater treatment from nitrile compounds. Biofilms of two species of nitrile hydrolyzing bacteria on basalt and carbon fibers effectively purify water from nitriles in a submerged packed-bed reactor. Supplementary Information: The online version contains supplementary material available at 10.1007/s12088-022-01030-z.

Prediction of early recurrencies of uterine body cancer based on monitoring of serum biological markers.

The aim of this work was to optimize the prognosis of early recurrences of uterine cancer by searching for informative laboratory biomarkers. The study included 343 patients with I-IV stages FIGO endometrial adenocarcinoma the ages of 53 and 76 years. In patients before surgery, as well as 3 and 6 months after the end of primary treatment, the concentration of oncological markers CA-125, HE4, DJ-1, DKK-1 was determined in blood serum by ELISA and electrochemiluminescence immunoassay. We analyzed the predictive informativeness of monitoring markers to assess the risk of early recurrences of cancer within two years after surgery. In patients with uterine cancer with the stage of the disease and the degree of tumor differentiation in the blood serum, the concentration of DJ-1 is changed. With myometrial invasion of more than 50%, increased serum levels of three markers - CA-125, HE4, and DJ-1. Depending on the risk of disease recurrence, the assessment of which was determined by intraoperative and histological characteristics, a difference was revealed in the content of HE4 and DJ-1 markers in the blood. It was found that the risk of early recurrence of uterine cancer increased (p<0,05) when the concentration of CA-125 exceeded the level of 29,3 U/ml, HE4 was above 79,3 pmol/l, DJ-1 was above 90,0 ng/ml and DKK-1 above 47,3 pg/ml 6 months after the end of primary treatment. The highest predictive information value was found for monitoring DJ-1 and HE4 in blood serum, which indicates that they are promising for use in continuous monitoring of the course of cancer.

[Differential bioinformational model for diagnostics of inflammatory and tumor diseases of the parotid salivary gland]. / Differentsial'naya bioinformatsionnaya model' diagnostiki vospalitel'nykh i opukholevykh zabolevanii okoloushnoi slyunnoi zhelezy.

THE AIM: Of the work was to develop a diagnostic algorithm for the differentiation of chronic inflammatory, benign and malignant processes in the parotid salivary gland (PSG) by the ratio of pro- and anti-inflammatory cytokines in the oral fluid. MATERIALS AND METHODS: The epidemiological group of patients with cancer of the parotid salivary gland included 140 people from the oncological register of the Rostov region with the date of diagnosis, from 1969 to 2020. The clinical part of the work was performed on 70 patients of both sexes aged 50 to 80 years: 15 patients with chronic nonspecific parenchymal sialadenitis of the PSG (ICD K11.2) (group 1), 19 patients with pleomorphic adenoma of the PSG (ICD D11.0) (2 group), 20 patients with cancer of the PSG (ICD C07) (group 3) and 16 healthy individuals without pathology of the oral cavity (control group). The concentration of interleukin-6 (IL-6) and interleukin-10 (IL-10) was determined in the oral fluid by enzyme immunoassay. RESULTS: It was found that in 58.5% of cases at the initial examination of patients with PSG cancer referred to a tertiary care hospital an erroneous opinion was formed about the inflammatory origin of the process. In inflammatory and tumor lesions of the PSG multidirectional differences are noted in the ratio between the concentrations of pro- and anti-inflammatory mediators in the oral fluid. In chronic sialadenitis of PSG in the oral fluid a moderate increase in the levels of IL-6 and IL-10 occurs, in the presence of adenoma of PSG, the concentration of IL-6 does not change while IL-10 increases threefold, and there is a sharp and unidirectional increase in the concentration of cytokines of the opposite groups in case of a malignant lesion of PSG. CONCLUSION: Comparison of the concentration of IL-6 and IL-10 in saliva and their ratio defined by the developed discriminant models helps to make an individual diagnostic decision in a specific clinical situation.

[The outcomes of different pancreatodigestive anastomoses in pancreatoduodenectomy].

AIM: To compare early results after pancreaticoduodenectomy depending on variant of pancreatico-digestive anastomosis. MATERIAL AND METHODS: It was analyzed early results of 207 pancreaticoduodenectomies for cancer which were performed for the period 2010-2014. Pancreatointestinal and pancreatogastric anastomoses were applied in 165 and 42 patients respectively. RESULTS: Complications were observed in 73 (44.2%) and 18 (38.3%) patients after pancreatointestinal and pancreatogastric anastomoses respectively. Six patients died after pancreatointestinal anastomosis. At the same time there were no deaths in the group of pancreatogastric anastomosis. Differences were significant. Postoperative hospital-stay was similar in both groups.

[Catalytic and Stereoselective Properties of the Immobilized Amidase of Rhodococcus rhodochrous 4-1].

The amidase of Rhodococcus rhodochrous 4-1 was immobilized by covalent attachment to activated chitosan by physical sorption on carbon adsorbents and by the formation of crosslinked aggregates in the absence of carrier material. Comparative analysis of particular catalytic properties of the free and chitosan-immobilized amidase was performed. It was shown that the enzyme retained 50-60% of its initial activity after covalent immobilization on chitosan and was characterized by increased temperature stability as compared to soluble amidase. Moreover, the immobilized enzyme retained more than 20% of its activity after five 24-h cycles of acrylamide deamination. The effects of different types of immobilization on amidase stereose-lective properties were studied by the model reaction of racemic lactamide hydrolysis to D-lactic acid and L-lactic acid. It was shown that crosslinked amidase aggregates possessed high D-stereoselectivity (up to 77-94%). The immobilized enzyme showed the highest enantioselectivity at 60 degrees C.

[Activated Sludge Bacteria Transforming Cyanopyridines and Amides of Pyridinecarboxylic Acids].

Species diversity of bacteria from the activated sludge of Perm biological waste treatment facilities capable of transformation of cyanopyridines and amides of pyridinecarboxylic acids was investigated. Enrichment cultures in mineral media with 3-cyanopyridine as the sole carbon and nitrogen source were used to obtain 32 clones of gram-negative heterotrophic bacteria exhibiting moderate growth on solid and liquid media with 3- and 4-cyanopyridine. Sequencing of the 16S rRNA gene fragments revealed that the clones with homology of at least 99% belonged to the genera Acinetobacte, Alcaligenes, Delftia, Ochrobactrum, Pseudomonas, Stenotrophomonas, and Xanthobacter. PCR analysis showed that 13 out of 32 isolates contained the sequences (-1070 bp) homologous to the nitrilase genes reported previously in Alcaligenes faecalis JM3 (GenBank, D13419.1). Nine clones were capable of nitrile and amide transformation in minimal salt medium. Acinetobacter sp. 11 h and Alcaligenes sp. osv transformed 3-cyanopyridine to nicotinamide, while most of the clones possessed amidase activity (0.5 to 46.3 mmol/(g h) for acetamide and 0.1 to 5.6 mmol/(g h) for nicotinamide). Nicotinamide utilization by strain A. faecalis 2 was shown to result in excretion of a secondary metabolite, which was identified as dodecyl acrylate at 91% probability.

[Coordinated aberranit expression of miRNAs in colon cancer].

Applying the method of multiple parallel sequencing on the MiSeq platform (Illumina, United States), a comparative analysis of miRNA expression in tumor and normal colon tissuie cells was performed. Forty miRNAs aberrantly expressed in cancer were detected. Among them, 15 and 25 miRNAs showed increased arid decreased expression, respectively, for all or most of the cases. Sixteen miRNA clusters were identified, which showed a coordinated or incompletely coordinated aberrant expression in colorectal cancer cells. In two (miR-183/182 and miR-106b/25) and four (miR-143/145, miR-497/195, miR-30e/30c-1, and miR-30a/30c-2) miRNA clusters, respectively, a statistically significant coordinated increase or decrease in expression was iegistered for all miRNAs withini the corresponding cluster. Three aberrantly expressed well-known miRNAs (miR-100-5p, mil-30d-5p, and miR-204-5p) were identified, which, however, had never 'before been associated with coloreictal cancer. The obtained results demonstrate the potential and promising application of 6 miRNA clusters with' coordinated aberrant expression as markers for colorectal cancer.

Extrathymic rearrangement of alphabetaT-lymphocyte antigen receptor genes during pregnancy.

The existence of alphabetaT-lymphocyte differentiation processes have been demonstrated in mouse peripheral lymphoid organs during pregnancy. Study of pregnant Swiss mice has shown that the development of the second half of gestation is accompanied by expression of RAG-1 recombinase mRNA and unrearranged TCR alpha-chain (pre-TCRalpha) preferentially in T-lymphocytes of lymph nodes involved in uterine drainage (para-aortal lymph nodes), and to a lesser extent in other lymph nodes (mainly from axillary lymph nodes). The data suggest that during pregnancy the differentiation of alphabetaT lymphocytes may occur not only in central (thymus) but also in peripheral lymphoid organs.