ABSTRACT
It was investigated the influence of pineal peptides on the aging changes both circadian rhythm of thymic serum factor (FTS) titer in male rats Wistar and circannual rhythms of FTS, the amount of stromal cells-precurcors (CFC-F), granulocyte-macrophage cells-precurcors (CFC-GM), CD4+, Mac-1+ and CD19+-cells in bone marrow of male mice CBA. Epithalamin was injected chronically beginning from 6 month in rats and 4 month in mice. In old animals the rhythmical disturbancers of the indices characterized by loss of fluctuations, displacement of seasonal acrophase, increased or diminished amplitude, inversion of rhythm and desynchronization. After epithalamin injections in rats of 11, 17, 27 months the FTS titer at night increased and in old mice of 23-24 months appeared the peak of hormone level in summer-autumn, restored the difference between the amount of CD4+, Mac-1+-cells in bone marrow in spring and autumn, increased CFC-GM amount in spring, diminished CFC-F in autumn and increased this indice in winter. Epithalon also diminished of CFC-GM and CFC-F amount in old mice in autumn. The retarding age-related disturbances of suprachiasmaticus nucleus of hypothalamus structure, the diminishing corticosterone and testosterone levels at night, the increasing hormones level in summer and the falling in winter plays role in the improvement of thymus and bone marrow rhythmical function in old animals, reseaved epithalamin. The rhythms of most indices rhythms in old animals showed a pattern of adult.
Subject(s)
Aging/physiology , Bone Marrow/physiology , Circadian Rhythm , Peptides/physiology , Thymic Factor, Circulating/metabolism , Thymus Gland/physiology , Animals , Bone Marrow/metabolism , Bone Marrow Cells/metabolism , CD4-Positive T-Lymphocytes/immunology , Circadian Rhythm/drug effects , Circadian Rhythm/physiology , Corticosterone/blood , Female , Granulocyte-Macrophage Colony-Stimulating Factor/analysis , Granulocyte-Macrophage Colony-Stimulating Factor/metabolism , Macrophages/drug effects , Macrophages/physiology , Male , Mice , Mice, Inbred CBA , Peptides/pharmacology , Pineal Gland/physiology , Rats , Rats, Wistar , Seasons , Stromal Cells/drug effects , Stromal Cells/physiology , Suprachiasmatic Nucleus/cytology , Testosterone/blood , Thymus Gland/metabolismSubject(s)
Aging/immunology , Bone Marrow Transplantation/immunology , T-Lymphocyte Subsets/immunology , Whole-Body Irradiation , Animals , CD4 Lymphocyte Count/radiation effects , Female , Immunologic Memory , Mice , Mice, Inbred CBA , T-Lymphocyte Subsets/radiation effects , Transplantation, IsogeneicABSTRACT
Femurs from both young and old mice were implanted subcutaneously to young syngeneic recipients. 2-2.5 months after surgery femoral marrow grafts were repopulated with nucleated cells and macrophage-granulocyte precursor cells irrespective of the donor's age. Marrow depletion of femurs prior to implantation caused a sharp decrease in hemopoietic repopulation of implants from old, but not young, donors. It is concluded that age-dependent functional peculiarities of stromal microenvironment can be visualized owing to the extraordinary local regenerative demand.
