ABSTRACT
AIM: To ascertain parameters of immune response significant for prognosis of the disease outcome in patients with acute hepatitis C (AHC). MATERIAL AND METHODS: Seventy two examinees were divided into three groups: 30 patients of group 1 had AHC; 29 patients of group 2 had chronic hepatitis C (CHC) with the disease history 3-10 years; 13 AHC convalescents who had the disease 3-10 years ago. The control group consisted of 10 healthy subjects. The following parameters of immune status were studied: CD3+, CD4+, CD8+, CD16+, CD20+, HLA-DR+CD3+, CD95+, O-lymphocytes. RESULTS: By clinicobiochemical picture and the results of 12-month follow-up RT-PCR investigations of blood HCV RNA, AHC patients were divided into two groups: in 18 (72%) of 25 patients AHC became chronic, 7 (28%) patients achieved convalescence. The proportion and absolute number of subpopulations of lymphocytes CD8+, CD20+, CD16+ as well as CD4/CD8 in patients with AHC with different outcomes did not differ from the controls. AHC convalescents and patients with AHC transformation into chronic hepatitis had in the acute period of the disease much higher absolute number of subpopulations of the lymphocytes CD3+, CD4+, HLA-DR+CD3+ vs the controls. In patients with chronic hepatitis C with elevated levels of AlAT, 3-10 year follow-up registered significantly higher absolute amount of CD8+, CD3+, CD4+, HLA-DR+CD3+ vs the controls. Only patients with AHC which later became chronic had for 6 months of the disease significantly elevated absolute number of O-lymphocytes vs the controls. CONCLUSION: A high absolute count of O-lymphocytes in AHC patients may indicate probable development of chronic hepatitis C.
Subject(s)
Antigens, CD/analysis , Hepacivirus/isolation & purification , Hepatitis C/diagnosis , Hepatitis C/immunology , Lymphocytes/immunology , Adolescent , Adult , Hepatitis C, Chronic/diagnosis , Hepatitis C, Chronic/immunology , Humans , Male , Middle Aged , Prognosis , RNA, Viral/bloodABSTRACT
High-specific and sensitive method of identification of mycobacteria human and bovine species by using PCR was created. The pair of primers from DNA sequence, coding protein MbaA was chosen. As a target for amplification was taken the sequence of DNA from 1796 to 2115 pb coding region of protein from 407 to 514 amino acid with lesser hydrophobicity index. That gave the possibility to choose the specific for M. tuberculosis--M. bovis part of DNA. Simple and effective method of preparing mycobacterial cells for DNA amplification was proposed.
Subject(s)
Mycobacterium bovis/isolation & purification , Mycobacterium tuberculosis/isolation & purification , DNA Primers , Epitopes , Genetic Code , Nucleic Acid Hybridization , Polymerase Chain Reaction , Sensitivity and SpecificityABSTRACT
An express method has been elaborated for identification of human and bovine species mycobacteria in the polymerase chain reaction. The high specificity of the technique and simplicity of material preparation for research make the experimental procedure simple and quick, permitting one to identify Mycobacterium tuberculosis and Mycobacterium bovis directly in pathogenic material.
