ABSTRACT
Sixty-seven therapeutic fibrobronchoscopies (FBS) were carried out in burnt patients with suspected inhalation injuries at Center for Burns at the Janelidze Institute of Emergency Care in 1996-1998. The severity of inhalation injury was assessed from the extent and severity of mucosal edema, spot hematomas and their type, erosions and ulcers, discharge, and ventilation obstruction. An original score is developed for evaluating the severity of inhalation injuries in burnt patients. Inhalation injuries were diagnosed in 63 patients. FBS was performed with tracheobronchial lavage with antibiotics, proteolysis enzymes, and repair stimulants. Mortality among burnt patients with inhalation injuries was decreased to 38%.
Subject(s)
Burns, Inhalation , Adjuvants, Immunologic/therapeutic use , Adolescent , Adult , Aged , Aged, 80 and over , Anti-Bacterial Agents/therapeutic use , Bronchoscopy , Burns, Inhalation/diagnosis , Burns, Inhalation/therapy , Female , Humans , Male , Middle Aged , Models, Theoretical , Peptide Hydrolases/therapeutic use , Prognosis , Respiratory Function Tests , Treatment OutcomeABSTRACT
Flow cytometry was used to demonstrate that cultured human melanoma BRO cells expressed membrane-bound tumour necrosis factor-alpha (TNF-alpha) and were able to release TNF-alpha upon treatment with glucosaminylmuramyl dipeptide (GMDP). The released TNF-alpha was shown to prime melanoma cells, previously unable to respond to GMDP by increasing expression of melanoma-associated antigens, making them sensitive to GMDP treatment.
Subject(s)
Acetylmuramyl-Alanyl-Isoglutamine/analogs & derivatives , Adjuvants, Immunologic/pharmacology , Melanoma/metabolism , Tumor Necrosis Factor-alpha/physiology , Acetylmuramyl-Alanyl-Isoglutamine/pharmacology , Animals , Antigens, Neoplasm/biosynthesis , Antigens, Neoplasm/drug effects , Cell-Free System/physiology , Culture Media, Conditioned/pharmacology , Flow Cytometry , Humans , Melanoma/immunology , Mice , Tumor Cells, CulturedSubject(s)
Acetylmuramyl-Alanyl-Isoglutamine/pharmacology , Adjuvants, Immunologic/pharmacology , Melanoma, Experimental/genetics , Neoplasm Metastasis/genetics , Phenotype , Animals , Antigens, Neoplasm/immunology , Antigens, Surface/immunology , Cytotoxicity, Immunologic , Humans , Melanoma, Experimental/immunology , Melanoma, Experimental/pathology , Mice , Mice, Nude , Neoplasm Metastasis/immunology , Neoplasm Transplantation , Transplantation, Heterologous , Tumor Cells, Cultured , Tumor EscapeSubject(s)
Melanoma/pathology , Mice, Nude/genetics , Animals , Disease Susceptibility/immunology , Genetic Predisposition to Disease , Humans , Melanoma/genetics , Melanoma/immunology , Mice , Mice, Nude/immunology , Neoplasm Metastasis , Neoplasm Transplantation , Neoplastic Cells, Circulating , Species Specificity , Tumor Cells, CulturedSubject(s)
Coronary Disease/diagnosis , Aged , Aged, 80 and over , Antihypertensive Agents/therapeutic use , Calcium Channel Blockers/therapeutic use , Cardiomyopathies/diagnosis , Coronary Disease/therapy , Diagnosis, Differential , Electrocardiography , Humans , Nitroglycerin/therapeutic use , RheologySubject(s)
Coronary Disease/diagnosis , Electrocardiography , Monitoring, Physiologic , Adult , Aged , Female , Humans , Male , Middle AgedABSTRACT
In 48 patients urgent operative interventions on organs of the abdominal cavity were performed against the background of the adequate central analgesia by phentanyl (0,02 mg/kg). The authors think the method to be inexpedient in operative interventions shorter than 60 min.
Subject(s)
Abdomen, Acute/surgery , Abdominal Injuries/surgery , Analgesia/methods , Anesthesia, Intravenous , Fentanyl/administration & dosage , Adolescent , Adult , Aged , Diazepam/administration & dosage , Droperidol/administration & dosage , Emergencies , Humans , Middle Aged , Preanesthetic MedicationABSTRACT
A comparative study of properties of soluble urease and urease immobilized by its binding to cellulose derivatives was carried out. Upon chemical binding of urease to cellulose derivatives the Mikhaelis constant and pH optimum for the enzyme action changed insignificantly. Maximum enzymic activity of soluble and immobilized urease occurred at 50 degrees C. The energy of activation of urea hydrolysis by urease during enzyme immobilization also changed insignificantly.
Subject(s)
Urease , Catalysis , Cellulose/analogs & derivatives , Enzymes, Immobilized , Hydrogen-Ion Concentration , KineticsABSTRACT
Effect of temperature, pH and univalent cation on kinetics of self-activation of B12-dependent glycerol dehydratase (GD) from Aerobacter aerogenes with Co alpha-[alpha-(5,6-dimethylbenzimidazolyl]-Co beta-adenosylcobamide (AdoCbl) was investigated. The activation energy of the process of GD inactivation is found to be 3.9 kkal/M, the effect of pH on GD inactivation being insignificant. Monovalent cation is not required for the formation of GD-AdoCbl complex, but it protects the complex from selfinactivation. The rate of GD inactivation greatly depends on concentration of monovalent cations. Effect of K+, Rb+, Cs+, Tl+ and NH4+ cations, which are enzyme cofactors, qualitatively differs from the effect of Na+ and Li+, which are inactive in a catalytic reaction. The presence of at least two cation-binding sites in GD molecule is suggested. Possible mechanism of the effect of environmental factors in self-inactivation of GD-AdoCbl complex is discussed.
