ABSTRACT
The level of glycated hemoglobin (GH) measured by the Diabetic test colorimetric method did not depend on the compensation. On the other hand, its level was increased in diabetes mellitus decompensation in comparison with subcompensation, if isoelectrofocussing of the studies hemolysates was carried out prior to colorimetry. Study of colorimetric migration of hemolysates showed that they were represented by a background which could distort the results.
Subject(s)
Glycated Hemoglobin/analysis , Calorimetry/methods , Calorimetry/standards , Diabetes Mellitus, Type 1/blood , Diabetes Mellitus, Type 1/diagnosis , Diabetes Mellitus, Type 2/blood , Diabetes Mellitus, Type 2/diagnosis , Evaluation Studies as Topic , Humans , Sensitivity and SpecificityABSTRACT
135 patients with diabetes mellitus were studied for the level of glycated hemoglobin (GH) with the aid of the colorimetric method diabetes test involving the use of 0.3 N oxaloacetic acid, 0.06 M thiobarbituric acid, colorimetry on the spectrophotometer with the wavelength of 443 nm. No correlation was established or only weak relationship between the level of GH and chief clinical manifestations of diabetes mellitus, such as indices for metabolism, clinical presentation of the illness, degree of microangiopathy. There was no correlation between the above method and isoelectrofocusing in capillaries. Little value can be attached to GH level in controlling diabetes mellitus.