ABSTRACT
The new synthesized Cu(II) chelates of amino acids Schiff bases were studied as a potential radioprotectors. Male albino rats of Wistar strain were exposed to X-ray whole-body irradiation at 4.8 Gy. This dose caused 30% mortality of the animals (LD30). The survival of animals exposed to radiation after preliminary administration of 10 mg/kg Cu(II)(Nicotinyl-L-Tyrosinate)2 or Cu(II)(Nicotinyl-L-Tryptophanate)2 prior to irradiation was registered about 80 and 100% correspondingly. Using spectrophotometric melting and agarose gel electrophoresis methods, the differences between the DNA isolated from irradiated rats and rats pretreated with Cu(II) chelates were studied. The fragments of DNA with different breaks were revealed in DNA samples isolated from irradiated animals. While, the repair of the DNA structure was observed for animals pretreated with the Cu(II) chelates. The results suggested that pretreatment of the irradiated rats with Cu(II)(Nicotinyl-L-Tyrosinate)2 and Cu(II)(Nicotinyl-L-Tryptophanate)2 compounds improves the liver DNA characteristics.
Subject(s)
Chelating Agents/chemistry , Copper/chemistry , DNA/chemistry , Radiation-Protective Agents/chemistry , Amino Acids/chemistry , Animals , Chelating Agents/administration & dosage , Copper/administration & dosage , Radiation , Rats , Schiff Bases/chemistryABSTRACT
The research deals with the effect of low-intensity 900 MHz frequency electromagnetic radiation (EMR), power density 25 µW/cm2, on the following rat brain and blood serum enzyme activities: creatine kinase (CK), playing a central role in the process of storing and distributing the cell energy, as well as alanine aminotransferase (ALT) and aspartate aminotransferase (AST) that play a key role in providing the conjunction of carbohydrate and amino acid metabolism. The comparative analysis of the changes in the enzyme activity studied at different times following the two-hour single, as well as fractional, radiation equivalent of the total time showed that the most radiosensitive enzyme is the brain creatine kinase, which may then be recommended as a marker of the radio frequency radiation impact. According to the analysis of the changing dynamics of the CK, ALT and AST activity level, with time these changes acquire the adaptive character and are directed to compensate the damaged cell energy metabolism.
Subject(s)
Alanine Transaminase/metabolism , Aspartate Aminotransferases/metabolism , Brain/radiation effects , Creatine Kinase/metabolism , Electromagnetic Radiation , Energy Metabolism/radiation effects , Adaptation, Physiological , Alanine Transaminase/blood , Animals , Aspartate Aminotransferases/blood , Brain/enzymology , Brain/metabolism , Creatine Kinase/blood , Dose-Response Relationship, Radiation , Male , Rats , Whole-Body IrradiationABSTRACT
To reveal protective role of the new Mn(II) complexes with Nicotinyl-L-Tyrosinate and Nicotinyl-L-Tryptophanate Schiff Bases against ionizing radiation. The DNA of the rats liver was isolated on 7, 14, and 30 days after X-ray irradiation. The differences between the DNA of irradiated rats and rats pre-treated with Mn(II) complexes were studied using the melting, microcalorimetry, and electrophoresis methods. The melting parameters and the melting enthalpy of rats livers DNA were changed after the X-ray irradiation: melting temperature and melting enthalpy were decreased and melting interval was increased. These results can be explained by destruction of DNA molecules. It was shown that pre-treatment of rats with Mn(II) complexes approximates the melting parameters to norm. Agarose gel electrophoresis data confirmed the results of melting studies. The separate DNA fragments were revealed in DNA samples isolated from irradiated animals. The DNA isolated from animals pre-treated with the Mn(II) chelates had better electrophoretic characteristics, which correspond to healthy DNA. Pre-treatment of the irradiated rats with Mn(II)(Nicotinil-L-Tyrosinate) and Mn(II)(Nicotinil-L-Tryptophanate)2 improves the DNA characteristics.
Subject(s)
Amino Acids/chemistry , DNA/metabolism , Liver/drug effects , Radiation, Ionizing , Schiff Bases/pharmacology , Animals , Calorimetry , Coordination Complexes/chemistry , Coordination Complexes/pharmacology , DNA/chemistry , DNA Damage/drug effects , Liver/metabolism , Liver/radiation effects , Male , Manganese/chemistry , Nicotinic Acids/chemistry , Rats , Rats, Wistar , Schiff Bases/chemistry , Tryptophan/analogs & derivatives , Tryptophan/chemistry , Tyrosine/analogs & derivatives , Tyrosine/chemistryABSTRACT
The protective effects of novel synthesized derivatives of some amino acids--nicotinyl-L-tyrosinate and nicotinyl-L-tryptophanate schiff bases and their Cu(II) and Mn(II) chelates on growth, survival and membrane-associated ATPase activity of E. coli under X-ray irradiation were investigated. The specific growth rate and survival of E. coli were decreased at 10, 20 and 30 Gy doses. However, as 30 Gy was found to be the most effective irradiation dose, it was chosen for studying the radio-protective properties of different compounds. These compounds could increase the bacterial cell protection against X-ray irradiation in concentration-dependent manner. They had a role in stimulation of synthesis or regulation of activity of metal-dependent enzymes, required for reversing the X-ray irradiation damage. The study may prove useful for further estimation of the effectiveness of different compounds as radio-protectors on bacteria and other cells, especially mammalian cells under X-ray irradiation.
