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J Struct Biol ; 206(1): 99-109, 2019 04 01.
Article in English | MEDLINE | ID: mdl-30502494

ABSTRACT

Solid state NMR (SSNMR) has earned a substantial success in the characterization of paramagnetic systems over the last decades. Nowadays, the resolution and sensitivity of solid state NMR in biological molecules has improved significantly and these advancements can be translated into the study of paramagnetic biomolecules. However, the electronic properties of different metal centers affect the quality of their SSNMR spectra differently, and not all systems turn out to be equally easy to approach by this technique. In this review we will try to give an overview of the properties of different paramagnetic centers and how they can be used to increase the chances of experimental success.


Subject(s)
Magnetic Resonance Spectroscopy/methods , Metalloproteins/chemistry , Metals/chemistry , Nuclear Magnetic Resonance, Biomolecular/methods , Organometallic Compounds/chemistry , Copper/chemistry , Iron/chemistry , Models, Molecular , Nickel/chemistry , Protein Conformation
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