ABSTRACT
A bacterial strain was isolated from an oil-contaminated site and on its' further characterization, exhibited the potential of synthesising metabolites and the ability to degrade crude oil. Its' morphological, biochemical and 16S rRNA analysis suggested that the bacterium belongs to Dietzia maris AURCCBT01. This strain rapidly grew in the medium supplemented with n-alkanes C14, C18, C20, C28 and C32 utilizing them as a sole carbon source and produced a maximum canthaxanthin pigment of 971.37 µg/L in the n-C14 supplemented medium and produced the lowest pigment yield of 389.48 µg/L in the n-C-32 supplemented medium. Moreover, the strain effectively degraded 91.87% of crude oil in 7 days. The emulsification activity of the strain was 25% with the highest cell surface hydrophobicity (70.26%) and it showed a decrease in surface tension, indicating that the biosurfactant production lowers the surface tension. This is the first report on the characterization of the strain, Dietzia maris AURCCBT01 and its' novelty of alkane degradation and simultaneous production of canthaxanthin pigment. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s13205-021-02807-7.
ABSTRACT
Novel benzimidazoquinoline derivative (AVT) was synthesized through a substitution reaction and characterized by various spectral techniques. Analyzing the optical properties of AVT under absorption and emission spectral studies in different environments exclusively with respect to solvents and pH, intriguing characteristics viz. aggregation induced emission enhancement (AIEE) in the THF solvent and 'On-Off' pH sensing were found at neutral pH. Sensing nature of AVT with diverse metal ions and bovine serum albumin (BSA) was also studied. Among the metal ions, Fe3+ ion alone tunes the fluorescence intensity of AVT probe in aqueous medium from "turn-on" to "turn-off" through ligand (probe) to metal charge transfer (LMCT) mechanism. The probe AVT in aqueous medium interacts strongly with BSA due to Fluorescence Resonance Energy Transfer (FRET) and the conformational change in BSA was further analyzed using synchronous fluorescence techniques. Docking study of AVT with BSA reveals that the active site of binding is tryptophan residue which is also supported by the experimental results. Interestingly, fluorescent AVT probe in cells was examined through cellular imaging studies using BT-549 and MDA-MB-231 cells. Thus, the single molecule probe based detection of multiple species and stimuli were described.
Subject(s)
Cytological Techniques/methods , Ferric Compounds/analysis , Fluorescence Resonance Energy Transfer/methods , Fluorescent Dyes/chemistry , Molecular Probes/chemistry , Quinolines/chemistry , Cell Line, Tumor , Ferric Compounds/chemistry , Fluorescent Dyes/analysis , Fluorescent Dyes/toxicity , Humans , Hydrogen-Ion Concentration , Molecular Probes/analysis , Molecular Probes/toxicity , Protein Binding , Quinolines/analysis , Quinolines/toxicity , Serum Albumin, Bovine/analysis , Serum Albumin, Bovine/chemistry , Spectrophotometry, UltravioletABSTRACT
In this work, the synthesis of silver nanoparticles from a pigment produced by a recently-discovered bacterium, Chryseobacterium artocarpi CECT 8497, was achieved, followed by an investigation of its anticancer properties. The bacterial pigment was identified as flexirubin following NMR ((1)H NMR and (13)C NMR), UV-Vis, and LC-MS analysis. An aqueous silver nitrate solution was treated with isolated flexirubin to produce silver nanoparticles. The synthesised silver nanoparticles were subsequently characterised by UV-Vis spectroscopy, Scanning Electron Microscopy (SEM), Energy Dispersive X-ray Spectroscopy (EDX), X-Ray Diffraction (XRD), and Fourier Transform Infrared (FTIR) Spectroscopy methodologies. Furthermore, the anticancer effects of synthesised silver nanoparticles in a human breast cancer cell line (MCF-7) were evaluated. The tests showed significant cytotoxicity activity of the silver nanoparticles in the cultured cells, with an IC50 value of 36µgmL(-1). This study demonstrates that silver nanoparticles, synthesised from flexirubin from C. artocarpi CECT 8497, may have potential as a novel chemotherapeutic agent.
Subject(s)
Antineoplastic Agents , Breast Neoplasms/drug therapy , Chryseobacterium/chemistry , Metal Nanoparticles/chemistry , Polyenes/chemistry , Antineoplastic Agents/chemistry , Antineoplastic Agents/pharmacology , Breast Neoplasms/metabolism , Female , Humans , MCF-7 CellsABSTRACT
Two independent mol-ecules of the title compound, C(17)H(12)ClN(3), are present in the structure. The angle between the planes defined by the atoms of the benzimidazole unit and the quinoline unit are 45.2â (3) and 44.0â (3)°, indicating an essentially identical conformation for both mol-ecules. Each of the independent mol-ecules is linked with a symmetry equivalent by an inter-molecular N-Hâ¯N hydrogen bond involving the two benzimidazole N atoms, to form chains in the crystallographic c direction.
ABSTRACT
In the title compound, C(20)H(19)N(3)O(3), the inter-planar angle between the benzimidazole unit and the quinoline unit is 25.1â (2)°. Two different hydrogen bonds involving the hydr-oxy group and the imidazole unit are present. An intra-molecular N-Hâ¯O hydrogen bond links the hydr-oxy group of the side chain with the imidazole unit, forming a 12-membered ring, and an inter-molecular O-Hâ¯N hydrogen bond links the mol-ecules, forming chains in the crystallographic b direction.
