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1.
Nat Commun ; 9(1): 3889, 2018 09 24.
Article in English | MEDLINE | ID: mdl-30250205

ABSTRACT

Granular aluminum (grAl) is a promising high kinetic inductance material for detectors, amplifiers, and qubits. Here we model the grAl structure, consisting of pure aluminum grains separated by thin aluminum oxide barriers, as a network of Josephson junctions, and we calculate the dispersion relation and nonlinearity (self-Kerr and cross-Kerr coefficients). To experimentally study the electrodynamics of grAl thin films, we measure microwave resonators with open-boundary conditions and test the theoretical predictions in two limits. For low frequencies, we use standard microwave reflection measurements in a low-loss environment. The measured low-frequency modes are in agreement with our dispersion relation model, and we observe self-Kerr coefficients within an order of magnitude from our calculation starting from the grAl microstructure. Using a high-frequency setup, we measure the plasma frequency of the film around 70 GHz, in agreement with the analytical prediction.

2.
Adv Gerontol ; 20(2): 106-15, 2007.
Article in Russian | MEDLINE | ID: mdl-18306698

ABSTRACT

Improving the status of elderly patients with diabetes mellitus is an important issue of gerontology and geriatrics. This work was aimed at the study of the effect of thymomimetic Vilon on the immune status and coagulation hemostasis in elderly patients with type I diabetes mellitus. It was found that the administration of Vilon as an addition to the complex therapy for this cohort of patients resulted in optimization of coagulation hemostasis, which was manifested in the increased content of natural anticoagulants: antithrombin III and protein C, as well as in the stimulation of fibrinolysis. In most cases Vilon reduced the dose of insulin necessary for the stabilization of carbohydrate metabolism. The content of T-helpers, T-dependent and non-T-dependent NK cells was reduced, and the level of active T-lymphocytes, B-lymphocytes and IgA was normalized, which points out the stabilizing effect of Vilon on the immune system and hemostasis.


Subject(s)
Aging , Blood Coagulation/drug effects , Diabetes Mellitus, Type 1 , Dipeptides/therapeutic use , Hypoglycemic Agents/therapeutic use , Immunity, Innate/drug effects , Insulin/therapeutic use , Adolescent , Adult , Aged , Aging/blood , Aging/immunology , Blood Glucose/analysis , Diabetes Mellitus, Type 1/blood , Diabetes Mellitus, Type 1/drug therapy , Diabetes Mellitus, Type 1/immunology , Dipeptides/administration & dosage , Drug Therapy, Combination , Female , Humans , Hypoglycemic Agents/administration & dosage , Insulin/administration & dosage , Male , Middle Aged , Severity of Illness Index , Treatment Outcome
5.
Genetika ; 25(11): 1925-36, 1989 Nov.
Article in Russian | MEDLINE | ID: mdl-2625199

ABSTRACT

Construction of a human cortex cDNA bank is described as well as the isolation from this bank of pBH71 and pBH3 clones with preferential expression in nervous and in tumor cells. The clones can be included into the third class of cDNA according to Sutcliff's classification. The mRNA corresponding to this cDNA class is considered to play the key role in determination of specificity of nervous tissue. Expression of the pBH71 sequence was revealed in human cortex and in tissues of different genesis (from neuroblastoma to uterus myoma), a 2 kb mRNA which corresponds to one and the same cDNA chain having been found in all tissues under analysis. The nucleotide sequence of cDNA insertion into the pBH71 clone of 447 n.p. was determined, and particular features of cDNA nucleotide composition and possible schemes of its translation were analysed. Weak homology was found between the 3'-end of cDNA insertion of the pBH71 clone and the 3'-end region of human proopiomelanocortine. The cDNA of the pBH3 clone hybridizes with the 0.8 kb mRNA revealed in human cortex and neuroendocrine tumors of different nature. No homology was revealed between the cDNA sequence of the pBH3 clone and any genes deciphered.


Subject(s)
Brain Chemistry , DNA/genetics , Gene Expression , Neoplasms/genetics , Nerve Tissue , Base Sequence , Gene Library , Humans , Molecular Sequence Data , Nucleic Acid Hybridization , Sequence Homology, Nucleic Acid
6.
Genetika ; 25(6): 1119-21, 1989 Jun.
Article in Russian | MEDLINE | ID: mdl-2509287

ABSTRACT

Expression of the c-fos proto-oncogene has been associated with mitosis or differentiation in a number of cultured cells or tissues in vivo. Expression of the c-fos proto-oncogene in adult rat brain cells was studied in the process of learning with the use of Northern hybridization techniques. Our results demonstrate that high levels of c-fos mRNA are detectable in brains of all animals treated. Therefore, c-fos is likely to play an important role in the process of learning.


Subject(s)
Cerebral Cortex/analysis , Gene Expression , Protein-Tyrosine Kinases/genetics , Proto-Oncogene Proteins/genetics , Proto-Oncogenes , Animals , Blotting, Northern , Cerebral Cortex/radiation effects , Male , Nucleic Acid Hybridization , Proto-Oncogene Proteins c-fos , RNA, Messenger/analysis , Rats
7.
FEBS Lett ; 212(2): 208-12, 1987 Feb 23.
Article in English | MEDLINE | ID: mdl-2434358

ABSTRACT

Two clones have been selected from a human fibroblast cDNA bank. By DNA sequencing the clones were shown to contain Alu elements located near the ends of the cDNA inserts. DNA of the clones was used for Northern blot hybridization analysis of a number of poly(A)-containing RNAs from normal human tissues (brain, stomach, uterus, spleen, fibroblasts) and tumors (neurinoma, glioma, neuroblastoma, liposarcoma, adrenal cortex adenocarcinoma). All RNA samples reveal a heterodisperse distribution of Alu transcripts with discrete bands in the region of 7-12 S RNA. The majority of these small poly(A)+ Alu+ RNAs contain Alu sequences only in one (canonical) orientation with functional signals including the split promoter for RNA polymerase III.


Subject(s)
Cloning, Molecular , DNA/analysis , DNA/metabolism , Poly A/genetics , RNA/genetics , Base Sequence , Cell Line , DNA Transposable Elements , Fibroblasts/metabolism , Humans , Neoplasms , Nucleic Acid Hybridization , Plasmids , RNA, Messenger , Transcription, Genetic
8.
Mol Biol (Mosk) ; 20(5): 1377-89, 1986.
Article in Russian | MEDLINE | ID: mdl-2430173

ABSTRACT

Alu containing cDNA clones were isolated from a human fibroblast cDNA library. The nucleotide sequences of two clones were determined. In both cases Alu repeats appeared to be situated in the regions presumably corresponding to the 3'-end of mRNA. The comparison of the unique sequences of those clones did not show any homology between them. Both cDNA clones contained large open reading frames, extending into the Alu regions. DNA of one clone was used as a probe for Northern blot-hybridization analysis of poly(A)+ cytoplasmic RNA from some normal and tumor human tissues. All RNA samples contained Alu-homologous transcripts, mainly in 7-12S fractions and only in one (canonical) orientation. This orientation contained functional regions including promotor region for RNA-polymerase III.


Subject(s)
DNA/genetics , Repetitive Sequences, Nucleic Acid , Cloning, Molecular , DNA, Neoplasm/genetics , Fibroblasts , Gene Expression Regulation , Humans , Nucleic Acid Hybridization , Plasmids , RNA/genetics , Tissue Distribution
16.
Biokhimiia ; 42(10): 1783-90, 1977 Oct.
Article in Russian | MEDLINE | ID: mdl-922066

ABSTRACT

Two general methods for the isolation of DNA from various sources based on the use of cetyltrimethylammonium bromide (cetavlon, CTA-Br) are described. Cetavlon is a strong cationic detergent precipitating DNA from diluted salt solutions. Cells are lysed and cellular components are dissolved in the presence of cetavlon, 5 M urea, 0.1 M EDTA and 2 M NaCl (KCl). In the first method pure DNA is precipitated in the form of CTA-salt by direct dilution of the lysate to bring the concentration of NaCl (KCl) down to 0.5 M after the removal of the main part of proteins by deproteinization with chloroform. In the first method pure DNA is precipitated in the form of CTA-salt by direct dilution of the lysate to bring the concentration of NaCl (KCl) down to 0.5 M after the removal of the main part of proteins by deproteinization with chloroform. In the second method DNA is purified on the hydroxyapatite column after cell lysis and the removal of cell debris by centrifugation. Both methods are suitable for rapid isolation of pure DNA from various sources with recovery about 80% and average molecular weight 20-10(6) and higher without use of ribonuclease, pronase and amylase.


Subject(s)
DNA/isolation & purification , Cetrimonium Compounds , Chemical Precipitation , Chromatography/methods , Hydroxyapatites , Methods
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