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1.
Micromachines (Basel) ; 14(6)2023 Jun 11.
Article in English | MEDLINE | ID: mdl-37374816

ABSTRACT

The article represents the design of two port-based printed MIMO antenna structures that have the advantages of low profile, simple structure, good isolation, peak gain, directive gain, and reflection coefficient. The performance characteristics are observed for the four design structures by cropping the patch region, loading the slits near the hexagonal-shaped patch, and adding and removing the slots in the ground area. The antenna provides a least reflection coefficient of -39.44 dB, a maximum electric field of patch region of 33.3 V/cm, a total gain of 5.23 dB, and good values of total active reflection coefficient and diversity gain. The proposed design provides nine bands' response, a peak bandwidth of 2.54 GHz, and a peak bandwidth of 26.127 dB. The four proposed structures are fabricated using a low-profile material to support mass production. The comparison among simulated and fabricated structures is included to check the authenticity of the work. The performance assessment of the proposed design with other published articles is carried out for the performance observation. The suggested technique is analyzed over the wideband of frequency region 1 GHz to 14 GHz. The multiple band responses make the proposed work suitable for wireless applications in S/C/X/Ka bands.

2.
R Soc Open Sci ; 10(6): 230282, 2023 Jun.
Article in English | MEDLINE | ID: mdl-37351487

ABSTRACT

Organic compound-based sensors are used in a variety of significant fields, including medical research, azeotropic calibration, vegetable oil extraction, the shoe industry and geothermal power plants. Here, a high-performance, two-dimensional material-based organic compound sensor has been proposed using a surface plasmon resonance (SPR) nanostructure consisting of a BK7 glass prism, Ag, BaTiO3, Ag, graphene and sensing layer. The reflectivity curves of the SPR device have been investigated when the sensing media are Pentane, n-Hexane, n-Heptane and n-Octane. The thickness of the BaTiO3 layer and the number of graphene sheets have been optimized to maximize the sensitivity. The highest sensitivity attained is 220.83 deg/RIU for n-Octane with 45 nm silver/10 nm BaTiO3/8 nm silver and four layers of graphene. We believe that the SPR-based sensors are simple and can replace the spectrometry, chromatography and electrochemical based sensors. The proposed design is extremely effective for diverse applications in biological, industrial and chemical detection because of its simple structure and great performance.

3.
Plasmonics ; : 1-8, 2023 May 10.
Article in English | MEDLINE | ID: mdl-37360049

ABSTRACT

Coronavirus disease (COVID-19) pandemic outbreak is being investigated by severe respirational syndrome coronavirus-2 (SARS-CoV-2) as a global health issue. It is crucial to propose sensitive and rapid coronavirus detectors. Herein, we propose a biosensor based on surface plasmon resonance (SPRE) for the detection of SARS-CoV-2 virus. To achieve improved sensitivity, a BiFeO3 layer is inserted between a metal (Ag) thin film and a graphene layer in the proposed SPRE device so that it has the structure BK7 prism/ Ag/ BiFeO3/ graphene/ analyte. It has been demonstrated that a small variation in the refractive index of the analyte can cause a considerable shift in the resonance angle caused by the remarkable dielectric properties of the BiFeO3 layer, which include a high index of refraction and low loss. The proposed device has shown an extremely high sensitivity of 293 deg/RIU by optimizing the thicknesses of Ag, BiFeO3, and the number of graphene sheets. The proposed SPRE-based sensor is encouraging for use in various sectors of biosensing because of its high sensitivity.

4.
Micromachines (Basel) ; 14(2)2023 Jan 31.
Article in English | MEDLINE | ID: mdl-36838056

ABSTRACT

We proposed a novel approach based on a complementary split-ring resonator metamaterial in a two-port MIMO antenna, giving high gain, multiband results with miniature size. We have also analyzed a circular disk metasurface design. The designs are also defected using ground structure by reducing the width of the ground plane to 8 mm and etching all other parts of the ground plane. The electric length of the proposed design is 0.5λ × 0.35λ × 0.02λ. The design results are also investigated for a different variation of complementary split-ring resonator ring sizes. The inner and outer ring diameters are varied to find the optimized solution for enhanced output performance parameters. Good isolation is also achieved for both bands. The gain and directivity results are also presented. The results are compared for isolation, gain, structure size, and the number of ports. The compact, multiband, high gain and high isolation design can apply to WiMAX, WLAN, and satellite communication applications.

5.
Micromachines (Basel) ; 14(1)2023 Jan 08.
Article in English | MEDLINE | ID: mdl-36677224

ABSTRACT

The manuscript represents a novel square tooth-enabled superstrate metamaterial loaded microstrip patch antenna for the multiple frequency band operation. The proposed tooth-based metamaterial antenna provides better gain and directivity. Four antenna structures are numerically investigated for the different geometry of the patch and tooth. These proposed structures are simulated, fabricated, measured, and compared for the frequency range of 3 GHz to 9 GHz. The electrical equivalent model of the split-ring resonator is also analyzed in the manuscript. The comparative analysis of all of the proposed structures has been carried out, in terms of several bands, reflectance response, VSWR, gain and bandwidth. The results are compared with previously published works. The effects are simulated using a high-frequency structure simulator tool with the finite element method. The measured and fabricated results are compared for verification purposes. The proposed structure provides seven bands of operation and 8.57 dB of gain. It is observed that the proposed design offers the multiple frequency band operation with a good gain. The proposed tooth-based metamaterial antenna suits applications, such as the surveillance radar, satellite communication, weather monitoring and many other wireless devices.

6.
Biosensors (Basel) ; 12(7)2022 Jul 18.
Article in English | MEDLINE | ID: mdl-35884338

ABSTRACT

MXene (Ti3C2Tx) has emerged very recently as an interacting material for surface plasmon resonance (SPR) configuration. It was discovered that Ti3C2Tx can facilitate the adsorption of biomolecules due to its higher binding energies, stronger interaction between matter and light, and larger surface area. In this work, a two-dimensional Ti3C2Tx and silicon layer-based SPR refractometric sensor is proposed for the sensitive and fast detection of milk fat concentration due to the high significance of this issue to people all over the world. The proposed SPR structure employs BK7 (BK7 is a designation for the most common Borosilicate Crown glass used for a variety of applications in the visible range) as a coupling prism and silver as a metal layer. The layer thicknesses and the number of Ti3C2Tx sheets are optimized for the highest performance. The highest reached sensitivity is 350 deg./RIU with 50 nm silver and 4 nm silicon with a monolayer of Ti3C2Tx, which is ultra-high sensitivity compared to the latest work that utilizes SPR configuration. The proposed SPR-based sensor's ultra-high sensitivity makes it more attractive for usage in a variety of biosensing applications.


Subject(s)
Silver , Surface Plasmon Resonance , Animals , Humans , Milk , Silicon/chemistry , Silver/chemistry , Surface Plasmon Resonance/methods
7.
Lab Chip ; 22(12): 2364-2375, 2022 06 14.
Article in English | MEDLINE | ID: mdl-35551303

ABSTRACT

There is a growing interest in developing personalized treatment strategies for each cancer patient, especially those with non-small cell lung carcinoma (NSCLC) which annually accounts for the majority of cancer related deaths in the US. Yet identifying the optimal NSCLC treatment strategy for each cancer patient is critical due to a multitude of mutations, some of which develop following initial therapy and can result in drug resistance. A key difficulty in developing personalized therapies in NSCLC is the lack of clinically relevant assay systems that are suitable to evaluate drug sensitivity using a minuscule amount of patient-derived material available following biopsies. Herein we leverage 3D printing to demonstrate a platform based on miniature microwells in agarose to culture cancer cell spheroids. The agarose wells were shaped by 3D printing molds with 1000 microwells with a U-shaped bottom. Three NSCLC cell lines (HCC4006, H1975 and A549) were used to demonstrate size uniformity, spheroid viability, biomarker expressions and drug response in 3D agarose microwells. Results show that our approach yielded spheroids of uniform size (coefficient of variation <22%) and high viability (>83% after 1 week-culture). Studies using epidermal growth factor receptor (EGFR) tyrosine kinase inhibitor (TKIs) drugs gefitinib and osimertinib showed clinically relevant responses. Based on the physical features, cell phenotypes, and responses to therapy of our spheroid models, we conclude that our platform is suitable for in vitro culture and drug evaluation, especially in cases when tumor sample is limited.


Subject(s)
Carcinoma, Non-Small-Cell Lung , Lung Neoplasms , Carcinoma, Non-Small-Cell Lung/pathology , Cell Line, Tumor , Drug Evaluation , Drug Resistance, Neoplasm , ErbB Receptors/metabolism , Humans , Lung Neoplasms/pathology , Mutation , Protein Kinase Inhibitors/therapeutic use , Sepharose
8.
Bioresour Technol ; 348: 126810, 2022 Mar.
Article in English | MEDLINE | ID: mdl-35131455

ABSTRACT

Design for fermentation bioreactor controllers is challenged by the nonlinear process kinetics and the lack of online measurements for key variables. This work developed a multi-input, multi-output advanced nonlinear control structure for a continuous, non-isothermal, constant volume fermentation bioreactor. Utilizing feedback linearization control for the bioreactor feed to regulate glucose concentration, and backstepping control for the cooling jacket feed to regulate reactor temperature. A developed novel estimator for biomass concentration was incorporated to provide online estimates for the unmeasurable state variable. Simulation results showed the control structure ability in efficiently establishing a combination of dynamic and fixed set points, despite disturbances in the bioreactor feed temperature and glucose concentration. Expanded bioreactor control authority increased operational flexibility and enhanced the potential for performance improvements. This work illustrated the effectiveness of feedback linearization and backstepping control in designing controllers for biological systems with nonlinear dynamics, complex interactions, and input disturbances.


Subject(s)
Bioreactors , Nonlinear Dynamics , Computer Simulation , Feedback , Fermentation
9.
J Biophotonics ; 15(5): e202200001, 2022 05.
Article in English | MEDLINE | ID: mdl-35103409

ABSTRACT

As a result of the risks that waterborne bacteria bring to the human body, identifying them in drinking water has become a global concern. In this article, a highly sensitive surface plasmon resonance (SPR) biosensor consisting of prism, Ag, graphene, affinity layer and sensing medium is proposed for rapid detection of the waterborne bacteria. Four SPR-based sensors are first studied with the structures prism/Ag/sensing medium, prism/Ag/affinity layer/sensing medium, prism/Ag/graphene/sensing medium, and prism/Ag/graphene/affinity layer/sensing medium. The latter structure is found to have the highest sensitivity so it is considered for further investigations. Four different commonly used prisms are then demonstrated which are N-FK51A, 2S2G, SF10 and BK7. The structure with the prism N-FK51A is found to correspond to the highest sensitivity so it is considered for further investigations. The structure parameters are then optimized. The proposed SPR sensor can achieve high sensitivity of about 221.63 °/RIU for Escherichia coli and 178.12 °/RIU for Vibrio cholera bacteria with an average value of 199.87 °/RIU. We believe that the proposed structure will open a new window in the field of microorganism detections.


Subject(s)
Biosensing Techniques , Graphite , Bacteria , Graphite/chemistry , Humans , Surface Plasmon Resonance
10.
Pediatr Cardiol ; 42(5): 993-1001, 2021 Jun.
Article in English | MEDLINE | ID: mdl-34047809

ABSTRACT

Unicuspid aortic valve (UAV) stenosis is a rare condition accounting for 5% of non-rheumatic aortic stenosis. The diagnosis can be difficult to make prior to surgical intervention and transesophageal echocardiography has been demonstrated to be more accurate in making the diagnosis compared to transthoracic echocardiography. The presence of a posteriorly located aortic orifice on the short-axis views, with one or two visible raphe anteriorly; the absence of commissures (acommissural); or the presence of a lone commissure (unicommissural) between the left and noncoronary, or the left and right cusps suggests the diagnosis. Patients with UAV are predominantly males and present with stenosis about a decade earlier than those with the more prevalent bicuspid aortic valves (BAV). They more commonly present with aortic annular dilatation and have fewer comorbidities at presentation compared to patients with BAV. Surgical management of UAV stenosis includes aortic valve replacement through standard open heart surgery or percutaneous transcatheter aortic valve replacement (TAVR), aortic valve repair either by bicuspidization, tricuspidization or trileaflet reconstruction, or the Ross procedure. Patients with UAV stenosis require less concomitant coronary or other cardiac procedures when they need surgical intervention, but are about a decade younger at the time of their death. UAV stenosis is a distinct congenital anomaly with a different natural course than BAV. Surgical management should be individualized based on the patient's age at presentation, aortoannular anatomy, and associated cardiac conditions.


Subject(s)
Constriction, Pathologic/surgery , Heart Defects, Congenital/surgery , Heart Valve Diseases/surgery , Transcatheter Aortic Valve Replacement/methods , Adult , Constriction, Pathologic/diagnostic imaging , Constriction, Pathologic/pathology , Echocardiography , Echocardiography, Transesophageal , Female , Heart Defects, Congenital/diagnosis , Heart Valve Diseases/diagnostic imaging , Heart Valve Diseases/pathology , Heart Valve Prosthesis , Humans , Male , Middle Aged
11.
Clin Cancer Res ; 26(16): 4339-4348, 2020 08 15.
Article in English | MEDLINE | ID: mdl-32430478

ABSTRACT

PURPOSE: Low-dose CT screening can reduce lung cancer-related mortality. However, CT screening has an FDR of nearly 96%. We sought to assess whether urine samples can be a source for DNA methylation-based detection of non-small cell lung cancer (NSCLC). EXPERIMENTAL DESIGN: This nested case-control study of subjects with suspicious nodules on CT imaging obtained plasma and urine samples preoperatively. Cases (n = 74) had pathologic confirmation of NSCLC. Controls (n = 27) had a noncancer diagnosis. We detected promoter methylation in plasma and urine samples using methylation on beads and quantitative methylation-specific real-time PCR for cancer-specific genes (CDO1, TAC1, HOXA7, HOXA9, SOX17, and ZFP42). RESULTS: DNA methylation at cancer-specific loci was detected in both plasma and urine, and was more frequent in patients with cancer compared with controls for all six genes in plasma and in CDO1, TAC1, HOXA9, and SOX17 in urine. Univariate and multivariate logistic regression analysis showed that methylation detection in each one of six genes in plasma and CDO1, TAC1, HOXA9, and SOX17 in urine were significantly associated with the diagnosis of NSCLC, independent of age, race, and smoking pack-years. When methylation was detected for three or more genes in both plasma and urine, the sensitivity and specificity for lung cancer diagnosis were 73% and 92%, respectively. CONCLUSIONS: DNA methylation-based biomarkers in plasma and urine could be useful as an adjunct to CT screening to guide decision-making regarding further invasive procedures in patients with pulmonary nodules.


Subject(s)
Carcinoma, Non-Small-Cell Lung/genetics , Cysteine Dioxygenase/genetics , Homeodomain Proteins/genetics , SOXF Transcription Factors/genetics , Tachykinins/genetics , Biomarkers, Tumor/blood , Biomarkers, Tumor/genetics , Biomarkers, Tumor/urine , Carcinoma, Non-Small-Cell Lung/blood , Carcinoma, Non-Small-Cell Lung/pathology , Carcinoma, Non-Small-Cell Lung/urine , Cysteine Dioxygenase/blood , Cysteine Dioxygenase/urine , DNA Methylation/genetics , Early Detection of Cancer , Female , Homeodomain Proteins/blood , Homeodomain Proteins/urine , Humans , Male , Middle Aged , Promoter Regions, Genetic/genetics , SOXF Transcription Factors/blood , SOXF Transcription Factors/urine , Tachykinins/blood , Tachykinins/urine
12.
Semin Thorac Cardiovasc Surg ; 29(3): 428-430, 2017.
Article in English | MEDLINE | ID: mdl-29195579

ABSTRACT

Leiomyosarcoma (LMS) is a mesenchymal tumor originating from the smooth muscle cells. LMS of the great vessels accounts for 60% of cases, with inferior vena cava being the most common site. Pulmonary vein LMS is an extremely rare subset that was first reported in 1939. LMS is an aggressive tumor, making surgical resection the treatment of choice. Herein, we present a rare case of pulmonary vein LMS extending into the left atrium, which was resected.


Subject(s)
Heart Atria/pathology , Leiomyosarcoma/pathology , Pulmonary Veins/pathology , Vascular Neoplasms/pathology , Echocardiography , Female , Heart Atria/diagnostic imaging , Heart Atria/surgery , Humans , Leiomyosarcoma/diagnostic imaging , Leiomyosarcoma/surgery , Magnetic Resonance Imaging , Middle Aged , Neoplasm Invasiveness , Pulmonary Veins/diagnostic imaging , Pulmonary Veins/surgery , Tomography, X-Ray Computed , Treatment Outcome , Vascular Neoplasms/diagnostic imaging , Vascular Neoplasms/surgery
14.
Ann Thorac Surg ; 103(4): e323-e325, 2017 Apr.
Article in English | MEDLINE | ID: mdl-28359488

ABSTRACT

We report a rare case of left atrial paraganglioma with a synchronous carotid body paraganglioma in a 30-year-old man with succinate dehydrogenase B gene mutation. The patient initially presented with a neck mass and palpitations. Laboratory test results showed elevated catecholamine levels. A cardiac paraganglioma was identified by computed tomography, meta-iodobenzylguanidine scintigraphy, and magnetic resonance imaging. Surgical resection of both paragangliomas were performed on two separate occasions. Serum and urine catecholamine levels returned to normal range. On follow-up, there was no recurrence of the cardiac paraganglioma. Radiotherapy was subsequently initiated for recurrence in the carotid body paraganglioma.


Subject(s)
Carotid Body Tumor/diagnosis , Carotid Body Tumor/therapy , Heart Neoplasms/diagnosis , Heart Neoplasms/therapy , Adult , Heart Atria , Humans , Male
15.
Pediatr Cardiol ; 38(5): 1080-1083, 2017 Jun.
Article in English | MEDLINE | ID: mdl-28214965

ABSTRACT

The technique for successful surgical correction of an anomalous origin of the right coronary artery from the opposite aortic cusp with an aberrant course between the aorta and pulmonary artery is illustrated in a symptomatic 62-year-old woman. The intramural course of the right coronary artery traversed the tip of the commissure between the anterior and posterior leaflets, and its repair entailed unroofing of the intramural segment from inside the aortic intima. This technique required resuspension of the overlying commissure to maintain optimal aortic valve leaflet coaptation and prevent aortic insufficiency. Modifications of this technique have been utilized by us whenever the intramural segment traversed behind the commissure. In these cases, partial or subtotal unroofing of the intramural segment was performed to preserve the integrity of the intima behind the overlying commissure. More recently, we have performed the surgical correction by probing the intramural segment within the aortic wall to its most anterior location and then performing a wide anterior unroofing in the aortic intima, and marsupializing the aortic and coronary intima to avoid dissection or intimal flap development. We favor utilizing these techniques of anatomic correction of the anomalous coronary to other techniques involving coronary artery bypass grafting of the anomalous coronary, especially in adult patients, as unroofing provides more lasting results.


Subject(s)
Aortic Valve/surgery , Cardiac Surgical Procedures/methods , Coronary Vessel Anomalies/surgery , Female , Humans , Middle Aged
16.
J Microsc Ultrastruct ; 4(4): 167-174, 2016.
Article in English | MEDLINE | ID: mdl-30023224

ABSTRACT

Helicobacter pylori (Hp) inhabits the stomach of > 50% of humans and has been established as a major etiological factor in the pathogenesis of chronic gastritis, gastric atrophy, peptic ulcer disease, gastric adenocarcinoma, and gastric mucosa-associated lymphoid tissue lymphoma. The aim of this study was to provide unequivocal information about Hp-associated gastritis grading according to the Sydney grading system and to compare the histopathological features with the endoscopic findings and anti-Hp immunoglobulin (Ig)G serological status. This analytical study was conducted on 157 patients with dyspeptic gastritis. All patients underwent esophagogastroduodenoscopy, and antrum and corpus biopsies were taken. Blood samples were obtained from all participants. Different stains were performed on formalin-fixed, paraffin-embedded tissue blocks that included hematoxylin and eosin and Giemsa stain for histopathological interpretation. The endoscopic findings of gastritis were observed in 120 patients and most of them showed hyperemia (80 patients), whereas seven patients had normal appearing gastric mucosa. Histologically variable numbers of mononuclear inflammatory cellular infiltrates were seen in 150 cases (95.5%). Most of them showed Grade 1 gastritis (80 patients), whereas Grades 2 and 3 were found in 43 and 27 biopsies, respectively. Hp colonization was observed in most of the examined biopsies (93.7%). Hp-IgG seropositivity was found in 80.9% of cases and 19.1% were seronegative. The relationship between endoscopic and histological findings was significant (p < 0.001).

17.
Bioresour Technol ; 170: 385-394, 2014 Oct.
Article in English | MEDLINE | ID: mdl-25156875

ABSTRACT

Strategies for the transition of municipal sludge anaerobic digestion from mesophilic to thermophilic were assessed and the long-term stability and performance of thermophilic digesters operated at a solids retention time of 30days were evaluated. Transition from 36°C to 53.3°C at a rate of 3°C/day resulted in fluctuation of the daily gas and volatile fatty acids (VFAs) production. Steady-state was reached within 35days from the onset of temperature increase. Transitions from either 36 or 53.3°C to 60°C resulted in relatively stable daily gas production, but VFAs remained at very high levels (in excess of 5000mg COD/L) and methane production was lower than that of the mesophilic reactor. It was concluded that in order to achieve high VS and COD destruction and methane production, the temperature of continuous-flow, suspended growth digesters fed with mixed municipal sludge should be kept below 60°C.


Subject(s)
Bioreactors , Digestion/physiology , Fatty Acids, Volatile/biosynthesis , Methane/biosynthesis , Methanosarcinales/metabolism , Sewage/microbiology , Temperature , Anaerobiosis , Biological Oxygen Demand Analysis , Methanosarcinales/genetics , Polymerase Chain Reaction , RNA, Ribosomal, 16S/genetics , Species Specificity , Time Factors
18.
J Trauma Acute Care Surg ; 76(2): 273-7; discussion 277-8, 2014 Feb.
Article in English | MEDLINE | ID: mdl-24458033

ABSTRACT

BACKGROUND: Studies reporting on penetrating thoracic trauma in the pediatric population have been limited by small numbers and implied differences with the adult population. Our objectives were to report on a large cohort of pediatric patients presenting with penetrating thoracic trauma and to determine age-related impacts on management and outcome through comparison with an adult cohort. METHODS: A Level I trauma center registry was queried between 2006 and 2012. All patients presenting with penetrating thoracic trauma were identified. Patient demographics, injury mechanism, injury severity, admission physiology, and outcome were recorded. Patients were compared, and outcomes were analyzed based on age at presentation, with patients 17 years or younger defining our pediatric cohort. RESULTS: A total of 1,423 patients with penetrating thoracic trauma were admitted during the study period. Two hundred twenty patients (15.5%) were pediatric, with 205 being adolescents (13-17 years) and 15 being children (≤ 12 years). In terms of management for the pediatric population, tube thoracostomy alone was needed in 32.7% (72 of 220), whereas operative thoracic exploration was performed in 20.0% (44 of 220). Overall mortality was 13.6% (30 of 220). There was no significant difference between the pediatric and adult population with regard to injury mechanism or severity, need for therapeutic intervention, operative approach, use of emergency department thoracotomy, or outcome. Stepwise logistic regression failed to identify age as a predictor for the need for either therapeutic intervention or mortality between the two age groups as a whole. However, subgroup analysis revealed that being 12 years or younger (odds ratio, 3.84; 95% confidence interval, 1.29-11.4) was an independent predictor of mortality. CONCLUSION: Management of traumatic penetrating thoracic injuries in terms of the need for therapeutic intervention and operative approach was similar between the adult and pediatric populations. Mortality from penetrating thoracic trauma can be predicted based on injury severity, the use of emergency department thoracotomy, and admission physiology for adolescents and adults. Children may be at increased risk for poor outcome independent of injury severity. LEVEL OF EVIDENCE: Epidemiologic study, level III.


Subject(s)
Hospital Mortality/trends , Thoracic Injuries/mortality , Thoracic Injuries/therapy , Wounds, Penetrating/mortality , Wounds, Penetrating/therapy , Adolescent , Adult , Age Factors , Cause of Death , Child , Child, Preschool , Cohort Studies , Combined Modality Therapy , Confidence Intervals , Female , Humans , Injury Severity Score , Logistic Models , Male , Middle Aged , Odds Ratio , Predictive Value of Tests , Prognosis , Registries , Retrospective Studies , Risk Assessment , Survival Analysis , Thoracic Injuries/diagnosis , Trauma Centers , Treatment Outcome , Wounds, Penetrating/diagnosis , Young Adult
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