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1.
J Sci Food Agric ; 97(14): 4721-4726, 2017 Nov.
Article in English | MEDLINE | ID: mdl-28369962

ABSTRACT

BACKGROUND: Lichens provide a large array of compounds with the potential for pharmaceutical development. In the present study, extracts from three previously undescribed North American lichen species were examined for antioxidant, antibacterial and anticancer activities. RESULTS: The results from this study demonstrated the following: (i) Acarospora socialis ethanol extract exhibited significant DPPH antioxidant scavenging activities, which were concentration dependent; (ii) acetone and ethyl acetate extracts of Xanthoparmelia mexicana inhibited Gram-positive bacteria but had no effect on Gram-negative bacteria; X. mexicana acetone extract yielded a minimum inhibitory concentration (MIC) of 20.9 µg mL-1 against Staphylococcus aureus, and 41.9 µg mL-1 against Enterococcus faecalis; (iii) acetone extract of Lobothallia alphoplaca inhibited growth of cultured breast cancer MCF-7 cells with an effective concentration (EC50 ) of 87 µg mL-1 ; the MCF-7 cell cycle appears arrested in the G2 phase, whereas the DNA synthesis cell cycle (S) may be inhibited. CONCLUSION: New lichen species that possess strong biological activities have been identified. These lichens comprise secondary metabolites that possess antioxidant, antibacterial and anticancer properties. © 2017 Society of Chemical Industry.


Subject(s)
Anti-Bacterial Agents/pharmacology , Antineoplastic Agents/pharmacology , Antioxidants/pharmacology , Lichens/chemistry , Plant Extracts/pharmacology , Anti-Bacterial Agents/analysis , Antineoplastic Agents/analysis , Antioxidants/analysis , Cell Proliferation/drug effects , Gram-Negative Bacteria/drug effects , Gram-Positive Bacteria/drug effects , Humans , Lichens/metabolism , MCF-7 Cells , North America
2.
Enzyme Microb Technol ; 73-74: 44-50, 2015 Jun.
Article in English | MEDLINE | ID: mdl-26002503

ABSTRACT

Demethylation of industrial lignin has been for long coveted as a pathway to the production of an abundant natural substitute for fossil-oil derived phenol. In an attempt to possibly identify a novel Kraft lignin-demethylating enzyme, we surveyed a collection of fungi by using selected ion flow tube-mass spectrometry (SIFT-MS). This method readily identifies methanol resulting from lignin demethylation activity. Absidia cylindrospora, and unidentified Cylindrocladium sp. and Aspergillus sp. were shown to metabolize lignin via different pathways, based on the HPLC analysis of lignin fragments. Of these three, Cylindrocladium and Aspergillus were shown to retain most of the lignin intact after 3 weeks in culture, while removing about 40% of the available methoxy groups. Our results demonstrate that after optimization of culture and lignin recovery methods, biological modification of Kraft lignin may be a feasible pathway to obtaining demethylated lignin for further industrial use.


Subject(s)
Fungal Proteins/metabolism , Fungi/metabolism , Lignin/metabolism , Mass Spectrometry/methods , Chromatography, High Pressure Liquid , Methanol/analysis , Methylation , Mycology/methods , Species Specificity , Ultrafiltration
3.
J Microbiol Methods ; 112: 40-5, 2015 May.
Article in English | MEDLINE | ID: mdl-25771342

ABSTRACT

Selected Ion Flow Tube Mass Spectrometry (SIFT-MS) was used to quantify methanol and other volatile compounds in the headspace of one bacterial and 12 fungal lignin-degrading microbial cultures. Cultures were grown in 250 mL Erlenmeyer flasks capped with aluminum foil containing 40 mL of nutrient media using Kraft lignin (0.3% w/v) as the sole carbon source. Analysis was done using SIFT-MS with H3O(+) and NO(+) precursors. Product ions were identified with multiple ion mode (MIM). Full scan (FS) mode was used to identify other compounds of interest. Absidia cylindrospora, Ischnoderma resinosum and Pholiota aurivella increased headspace methanol concentration by 136 ppb, 1196 ppb and 278 ppb, respectively, while Flammulina velutipes and Laetiporus sulphureus decreased concentration below ambient levels. F. velutipes and L. sulphureus were found to produce products of methanol oxidation (formaldehyde and formic acid) and were likely metabolizing methanol. Some additional unidentified compounds generated by the fungal cultures are intriguing and will require further study. SIFT-MS can be used to quantify methanol and other volatile compounds in the headspace of microbial cultures and has the potential to be a rapid, sensitive, non-invasive tool useful in elucidating the mechanisms of lignin degradative pathways.


Subject(s)
Bacteria/metabolism , Fungi/metabolism , Lignin/metabolism , Mass Spectrometry/methods , Volatile Organic Compounds/analysis , Bacteria/growth & development , Carbon/metabolism , Culture Media/chemistry , Fungi/growth & development
4.
Rapid Commun Mass Spectrom ; 24(6): 815-22, 2010 Mar.
Article in English | MEDLINE | ID: mdl-20187085

ABSTRACT

The reactions of H(3)O(+), NO(+), and O(2) (+) with nineteen ester compounds occurring naturally in plants, and having important flavourant properties, were examined using selected ion flow tube mass spectrometry (SIFT-MS). The H(3)O(+) reactions primarily generate [R(1)COOR(2).H](+), and may also produce [R(2)](+) fragment ions and/or fragmentation within the ester linkage. Collisional association/adduct ions, [R(1)COOR(2).NO](+), are the main products formed in the NO(+) reactions, although the carboxyl fragment ion is also detected frequently. The identification of the parent compound may be made more easily in the H(3)O(+) and NO(+) reactions. The inclusion of O(2) (+) reactions in the analysis provides additional information, which may be applied when the identity of a parent compound cannot be determined solely from the H(3)O(+) and NO(+) analysis. Consideration of the product ions generated with the three precursors suggests that SIFT-MS can differentiate between many of the esters investigated, including isomers, although the product ions generated in the reactions with some esters are too similar to allow independent quantification. Our data therefore suggest that SIFT-MS may be a useful tool to rapidly analyse and quantify flavourant esters in complex gas mixtures.


Subject(s)
Esters/chemistry , Mass Spectrometry/methods , Nitrogen Oxides/chemistry , Onium Compounds/chemistry , Oxygen/chemistry , Gases/chemistry , Kinetics , Molecular Weight , Volatile Organic Compounds/chemistry
5.
Plant Physiol ; 128(3): 1149-62, 2002 Mar.
Article in English | MEDLINE | ID: mdl-11891269

ABSTRACT

The 20S proteasome (multicatalytic proteinase) was purified from maize (Zea mays L. cv DEA 1992) roots through a five-step procedure. After biochemical characterization, it was shown to be similar to most eukaryotic proteasomes. We investigated the involvement of the 20S proteasome in the response to carbon starvation in excised maize root tips. Using polyclonal antibodies, we showed that the amount of proteasome increased in 24-h-carbon-starved root tips compared with freshly excised tips, whereas the mRNA levels of alpha 3 and beta 6 subunits of 20S proteasome decreased. Moreover, in carbon-starved tissues, chymotrypsin-like and caseinolytic activities of the 20S proteasome were found to increase, whereas trypsin-like activities decreased. The measurement of specific activities and kinetic parameters of 20S proteasome purified from 24-h-starved root tips suggested that it was subjected to posttranslational modifications. Using dinitrophenylhydrazine, a carbonyl-specific reagent, we observed an increase in carbonyl residues in 20S proteasome purified from starved root tips. This means that 20S proteasome was oxidized during starvation treatment. Moreover, an in vitro mild oxidative treatment of 20S proteasome from non-starved material resulted in the activation of chymotrypsin-like, peptidyl-glutamyl-peptide hydrolase and caseinolytic-specific activities and in the inhibition of trypsin-like specific activities, similar to that observed for proteasome from starved root tips. Our results provide the first evidence, to our knowledge, for an in vivo carbonylation of the 20S proteasome. They suggest that sugar deprivation induces an oxidative stress, and that oxidized 20S proteasome could be associated to the degradation of oxidatively damaged proteins in carbon starvation situations.


Subject(s)
Carbon/deficiency , Cysteine Endopeptidases/metabolism , Multienzyme Complexes/metabolism , Plant Roots/enzymology , Zea mays/enzymology , Carbon/pharmacology , Chymotrypsin/metabolism , Cysteine Endopeptidases/genetics , Cysteine Endopeptidases/isolation & purification , Gene Expression Regulation, Enzymologic/drug effects , Gene Expression Regulation, Plant/drug effects , Hydrazines/pharmacology , Hydrogen-Ion Concentration , Multienzyme Complexes/genetics , Multienzyme Complexes/isolation & purification , Oxidation-Reduction , Oxidative Stress , Plant Roots/genetics , Proteasome Endopeptidase Complex , RNA, Messenger/drug effects , RNA, Messenger/genetics , RNA, Messenger/metabolism , Substrate Specificity , Temperature , Trypsin/metabolism , Zea mays/genetics
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