Therapeutic potentials of the caffeine in polycystic ovary syndrome in a rat model: Via modulation of proinflammatory cytokines and antioxidant activity.

Recent studies have shown that polycystic ovary syndrome (PCOS) affects about 6% of women worldwide. It is associated with reproductive and metabolic dysfunction. Caffeine is naturally found in tea, cocoa, and coffee. It has been shown that caffeine can change hormonal profiles, stimulate ovulation, and enhance fertility. Therefore, in this study, the effects of caffeine on rats with PCOS were investigated. For this purpose, 40 female rats were divided into five groups: (1) control group (without any intervention), (2) sham group (administration of olive oil as a caffeine solvent), (3) PCOS group (injection of 2 mg of estradiol valerate for each rat), (4) caffeine group (administration of 37.5 mg/kg caffeine for each rat), and (5) PCOS + caffeine group. After 21 days of treatment, the ovaries of rats were removed and prepared for further evaluations, including hematoxylin and eosin staining, TUNEL assay, real-time PCR, and biochemical analysis. Administration of caffeine in PCOS mice considerably reduced both the volume of the ovary (P < 0.05) and follicular clusters (P < 0.01). However, superoxide dismutase and glutathione peroxidase were dramatically active in the PCOS + caffeine group compared to others (P < 0.05). Besides, caffeine treatment in PCOS mice led to Bax reduction and increased Bcl-2 expression. On the other hand, the expression of IL-6 and TNF-α in PCOS + caffeine group was high compared to other groups. We found that caffeine can reduce apoptosis and inflammation in PCOS ovaries and enhance the unpleasant symptoms of PCOS.

The effect of natural infection with different Leptospira interrogans serovars on oxidative stress biomarkers and acute-phase responses in horses and cattle.

Leptospirosis is one of the important zoonotic bacterial diseases with a worldwide distribution that is often subclinical. We aimed to investigate the oxidant/antioxidant balance and acute-phase response in naturally infected horses and cattle with Leptospira interrogans. A total of 600 serum samples from horses and cattle were examined for L. interrogans antibodies using the microscopic agglutination test to determine anti-Leptospira IgG antibodies against a panel of eight important Leptospira antigens in Iran. Then, serum total antioxidant capacity, catalase, glutathione peroxidase, and malondialdehyde activities, and nitric oxide, total protein, serum amyloid A, haptoglobin, and albumin concentrations were measured in seropositive and seronegative samples. Serum catalase activities and malondialdehyde, serum amyloid A, and haptoglobin |concentrations in seropositive cattle and horses were significantly higher (P < .05) than in those that were seronegative. Antibody titers increased from 1:100 to ≥ 1:200 in cattle with L. interrogans infection, resulting in a decrease in the serum total antioxidant capacity (P < .05), an increase in serum glutathione peroxidase (P < .01) activity and nitric oxide (P < .05) , serum amyloid A (P < .01), and haptoglobin (P < .05) concentrations. Following the increase in the agglutinating antibody titers in horses infected with L. interrogans, the serum total antioxidant capacity (P < .01) decreased, and serum nitric oxide (P < .05), malondialdehyde (P < .05), and serum amyloid A (P < .01) concentrations were increased. In this study, horses and cattle had extensive changes in oxidant/antioxidant equilibrium and acute-phase protein concentrations when infected with L. interrogans. We also demonstrated a direct link between antibody titers and the type of leptospiral serovar using serum oxidative and inflammatory markers.

Temporospatial variation and health risk assessment of trihalomethanes (THMs) in drinking water (northwest Iran).

Trihalomethanes (THMs) are one of the most common classes of disinfection by-products. In this study, the temporospatial trends and health risks due to exposure to THMs in the Tabriz water distribution network were investigated. THM series were analyzed using gas chromatography equipped with electron capture detector. The non-carcinogenic and carcinogenic risks due to exposure to THMs were calculated using Monte Carlo simulations. Mean concentrations of THMs in winter and spring were 10.2 ± 9.3 µg/l and 252 ± 185.9 µg/l, respectively. More than 80% of THMs identified were bromodichloromethane. The mean values of lifetime cancer risk (LTCR) of THMs were calculated as 4.23E-06 and 2.38E-04 for winter and spring, respectively. This study showed that there were noticeable levels of THMs in Tabriz water distribution network, especially in the center of the city. Although the non-cancer risk through THMs was below permissible recommended levels, the cancer risk likely remains due to high levels of THMs in some locations.

First indoor radon mapping and assessment excess lifetime cancer risk in Iran.

Radon (222Rn) is believed to be the main contributor to lung cancer second to smoking. The first national indoor radon map derived from some scattered regional radon surveys in Iran. The arithmetic mean of indoor radon concentration was calculated to 117.4 ±â€¯97.7 Bq/m3. The mean excess life time cancer risk (ELCR) values were found to be in the range of 0.1%-4.26%, with an overall average value of 1.01%. The mean radon-induced lung cancer risk was 46.8 per million persons. Absence of sufficient indoor radon data showed that national wide monitoring programs should be activated in uncovered areas. Meanwhile, in order to provide further baseline values for radon mapping, we attempted to survey the radon levels inside 50 dwellings of Shabestar County in northwest of Iran. The investigation was also focused on the effects of some buildings related variables. The radon levels recorded varied from 3.92 to 520.12 Bq/m3, with a mean value of 56.19 ±â€¯45.96 Bq/m3. In 9% of dwellings radon concentration exceeded 100 Bq/m3, the limit recommended by the World Health Organization. The average annual effective dose received by the residents of studied area was calculated to be 1.4 mSv. The ELCR was estimated to be 0.54%.

Investigation of aerosols pollution across the eastern basin of Urmia lake using satellite remote sensing data and HYSPLIT model.

BACKGROUND: Desiccation of the world's second hypersaline lake, Lake Urmia, has drawn attention toward the feasibility of particle emissions from the lakebed to downwind regions. Therefore, this research was conducted to study spatiotemporal variations of aerosols across the eastern basin of the lake during 2001 to 2016. METHODS: The Aerosol Optical Depth (AOD) products of Moderate-resolution Imaging Spectroradiometer (MODIS), extracted from Terra platform for 999 rural and urban areas and compared over adjacent (Shabestar, Osku, Azarshahr, Ajabshir, Bonab, Malekan), middle (Tabriz and Maragheh) and far (Bostanabad, Heris, and Sarab) counties. Number of dusty days and direction of high wind speeds (≥ 11 m/s) were acquired from the East Azerbaijan Meteorological Organization and analyzed. Moreover, performing the backward trajectory model, the origin and distribution of aerosols were determined at altitudes of 500, 1000 and 2000 m. RESULTS: The spatiotemporal variations of AOD provided statistically significant correlations (R2 ≥ 0.5 and p < 0.05) against the number of dusty days. AOD value was higher between 2009 and 2016 and estimated to be 0.36, 0.33 and 0.31 over adjacent, middle and far areas, respectively. Analysis of wind direction and trajectory plots implied that the particulate matter (PM) of study area was mainly transported from Iraq and Syria, especially in April, May and June months. CONCLUSIONS: PM has followed an increasing trend, while the adjacent areas have experienced higher pollution compared to far counties. The southwestern winds can play an important role in transportation of aerosols from either lakebed or western countries to the study area.