Comparison of bacterial microleakage of three bioactive endodontic sealers in simulated underwater diving and aviation conditions.

BACKGROUND: Bacterial microleakage is an important cause of apical periodontitis and endodontic treatment failure. This study aimed to assess the bacterial microleakage of nano-mineral trioxide aggregate (nano-MTA) as a sealer, Endoseal MTA, and GuttaFlow Bioseal sealers in atmospheric pressure, and simulated underwater diving and aviation conditions. METHODS: In this in vitro, experimental study, 180 extracted single-rooted teeth were cleaned and shaped, and were then randomly divided into three groups for single-cone obturation using Endoseal MTA, GuttaFlow Bioseal, or nano-MTA as a sealer. Each group was then randomly divided into three subgroups, and subjected to ambient atmospheric pressure, 2 atm pressure (to simulate underwater diving), and 0.5 atm pressure (to simulate aviation) using a custom-made pressure chamber. The teeth then underwent microbial leakage test using Streptococcus mutans (S. mutans), and the percentage of samples showing microleakage was recorded for up to 1 month, and analyzed using the Chi-square test. RESULTS: The three sealer groups were significantly different regarding bacterial microleakage (P < 0.05). The nano-MTA group showed significantly higher microleakage after 15 days than the other two groups (P = 0.006). The effect of pressure on bacterial microleakage was not significant in any sealer group (P > 0.05). CONCLUSION: Within the limitations of this in vitro study, it may be concluded that single-cone obturation technique using nano-MTA as a sealer results in lower resistance to bacterial microleakage compared with the use of GuttaFlow Bioseal, and Endoseal MTA. Pressure changes in simulated underwater diving and aviation conditions had no significant effect on bacterial microleakage. Trial Registration Number This is not a human subject research.

Intraoperative Blood Loss in Maxillofacial Trauma Surgery.

BACKGROUND AND OBJECTIVES: This study is aimed to assess the intraoperative blood loss of patients with maxillofacial bone fracture surgical intervention and to assess their need for blood transfusion. MATERIALS AND METHODS: In this retrospective study, intraoperative blood loss of 206 patients with facial bone fractures, who underwent surgery between 2017 and 2018, was retrieved. The patient's demographic information, the amount of blood loss, the type of facial bone fracture, and the patient's requirement for blood products were evaluated. RESULTS: The average amount of intraoperative blood loss was 77.6 ml, and none of the patients required a blood transfusion during the operation in this group of patients. maxillofacial bone fracture was most common in young males. The leading cause of fractures was motor vehicle accident. There was a reverse correlation between the patient's age and surgical blood loss, but there was no statistically significant relationship between the patient's gender and intraoperative surgical blood loss. CONCLUSION: The results of the present study show that preparation for blood transfusion in patients with maxillofacial traumatic bone fracture requiring surgery is uncommon if patients have no systemic disease or specific blood dyscrasias preoperatively.