ABSTRACT
BACKGROUND: Type II diabetes is on the rise while obesity is one of the strongest risk factors of type II diabetes. The search for a drug for type II that can equally mitigate obesity related complication is desired. METHODS: The acetone leaf extract of Senna italica was evaluated for its cytotoxic, antiglycation, and lipolytic effect, glucose uptake, and GLUT4 translocation and expression using published methods, while that for adipogenesis and protein expression levels of obesity related adipokines was assessed using adipogenesis assay and mouse adipokine proteome profiler kit, respectively. The possible mechanism of glucose uptake was assessed through the inhibition of PI3K pathway. RESULTS: The extract had no adverse effect on 3T3-L1 cell viability (CC50 > 1000 µg/ml). High antiglycation effect was attained at 10 mg/ml, while at 25-200 µg/ml it showed no significant increase in adipogenesis and lipolysis. The extract at 100 µg/ml was shown to decrease the expression levels of various adipokines and minimal glucose uptake at 50-100 µg/ml with a nonsignificant antagonistic effect when used in combination with insulin. GLUT4 translocation and expression were attained at 50-100 µg/ml with an increase in GLUT4 expression when in combination with insulin. CONCLUSION: The acetone leaf extract of S. italica stimulates glucose uptake through the PI3K-dependent pathway and can serve as a source of therapeutic agent for the downregulation of obesity-associated adipokines in obesity and antiglycation agents.
