Aluminium stress modulates the osmolytes and enzyme defense system in Fagopyrum species.

The present investigation describes aluminum-induced changes in the leaves of two buckwheat species using both physiological and biochemical indices. With increasing levels of Al (viz. 100, 200 and 300 µM), the mean length of root, shoot as well as their biomass accumulation decreased linearly with respect to control. Tolerance test of F. kashmirianum revealed that it was more tolerant to Al-stress than F. tataricum as revealed by higher accumulation of Al in its roots without any significant damage. Translocation factor (TF) values of both species were found to be < 1, indicating more Al is restrained in roots. Total chlorophyll showed a non-significant increase in F. tataricum while as decreased in F. kashmirianum at 300 µM concentration besides, the carotenoid content exhibited inclined trend in F. tataricum and showed a concomitant decrease in F. kashmirianum. The anthocyanin level showed a non-significant decline in F. kashmirianum. Exposure to different Al-treatments enhances malondialdehyde (MDA), H2O2 and membrane stability index (MSI) in both species, with increases being greater in F. kashmirianum than F. tataricum as also revealed by DAB-mediated in vivo histo-chemical detection method. The osmolyte level in general were elevated in both buckwheat species however, enhancement was more in F. tataricum than F. kashmirianum. The activities of antioxidant enzymes viz. superoxide dismutase (SOD), ascorbate peroxidase (APX), guaiacol peroxidase (POD), glutathione reductase (GR), glutathione-S-transferase (GST) were positively correlated with Al-treatment except catalase (CAT) which exhibits a reverse outcome in F. kashmirianum. The present investigation could play an essential role to better understand the detoxification mechanisms of Al in plants.

Metagenomic analysis of uncultured microorganisms and their enzymatic attributes.

Although second generation biofuel technology is a sustainable route for bioethanol production it is not currently a robust technology because of certain hindrances viz., unavailability of potential enzyme resources, low efficiency of enzymes and restricted availability of potent enzymes that work under harsh conditions in industrial processes. Therefore, bioprospecting of extremophilic microorganisms using metagenomics is a promising alternative to discover novel microbes and enzymes with efficient tolerance to unfavourable conditions and thus could revolutionize the energy sector. Metagenomics a recent field in "omics" technology enables the genomic study of uncultured microorganisms with the goal of better understanding microbial dynamics. Metagenomics in conjunction with NextGen Sequencing technology facilitates the sequencing of microbial DNA directly from environmental samples and has expanded, and transformed our knowledge of the microbial world. However, filtering the meaningful information from the millions of genomic sequences offers a serious challenge to bioinformaticians. The current review holds the opinion tool 'know- how' to unravel the secrets of nature while expediting the bio-industrial world. We also discuss the novel biocatalytic agents discovered through metagenomics and how bioengineering plays a pivotal role to enhance their efficiency.

Characterization of mercury-induced stress biomarkers in Fagopyrum tataricum plants.

The effect of mercury stress on antioxidant enzymes, lipid peroxidation, photosynthetic pigments, hydrogen peroxide content, osmolytes, and growth parameters in Tartary buckwheat were investigated. The effect of Hg-exposure was found to be time (15 and 30 days) and concentration (0, 25, 50, and 75 µM) dependent. Hg was readily absorbed by seedlings with higher content in roots and it resulted in reduction of root and shoot length. The root and shoot Hg uptakes were significantly and directly correlated with each other. However, the fresh mass and biomass increased up to 50 µM Hg-treatment at both time periods. A significant positive correlation was observed between biomass accumulation with relative water content. Hg levels were positively correlated with the production of hydrogen peroxide in leaves as evidenced by 3, 3-diaminobenzidine (DAB)-mediated tissue fingerprinting. The osmolyte levels in general were elevated except for proline and protein which showed a decline at 75 µM Hg-treatment at 30-days. Amongst the photosynthetic pigments, chlorophyll showed a decline while as carotenoid and anthocyanin levels were elevated. The activity of antioxidant enzymes such as ascorbate peroxidase (APX), guaiacol peroxidase (POD), glutathione reductase (GR), Glutathione-s-transferase (GST) and superoxide dismutase (SOD) were positively correlated with Hg-treatment except SOD, which declined at 75 µM Hg-treatment in 30-days old seedlings. Catalase (CAT) activity showed a positive correlation up to 50 µM Hg-treatment but at 75 µM Hg-stress it decreases at both 15 and 30 days.