Octopamine is involved in TRP-induced thermopreference responses in American cockroach.

Insects' thermoregulatory processes depend on thermosensation and further processing of thermal information in the nervous system. It is commonly known that thermosensation involves thermoreceptors, including members of the TRP receptor family, but the involvement of neurotransmitters in thermoregulatory pathways remains unstudied. We conducted test to determine whether octopamine, a biogenic amine that acts as a neurotransmitter and neurohormone in insects, is involved in TRP-induced thermoregulatory responses in Periplaneta americana. We used capsaicin, an activator of the heat-sensitive TRP channel, Painless, to induce thermoregulatory response in cockroaches. Then, we evaluated the behavioural (thermal preferences and grooming), physiological (heart rate) and biochemical responses of insects to capsaicin, octopamine and phentolamine - octopaminergic receptor blocker. Capsaicin, similar to octopamine, increased cockroaches' grooming activity and heart rate. Moreover, octopamine level and protein kinase A (PKA) activity significantly increased after capsaicin treatment. Blocking octopaminergic receptors with phentolamine diminished cockroaches' response to capsaicin - thermoregulatory behaviour, grooming and heart rate were abolished. The results indicate that octopamine is a neurotransmitter secreted in insects after the activation of heat receptors.

On the Efficiency of Laser Alloying of Grey Cast Iron with Tungsten and Silicon Carbides.

Cast iron is widely used in engineering production and in the surface alloying of workpieces, which is exploited to improve the properties of the material. Research on cast iron is still valid and needed for the manufacturing processes throughout the product life cycle. In this study, the gray, cast iron GJL 200 laser processing is described based on surface alloying with WC and SiC particulates. SEM analysis and XRD analysis, as well as microhardness testing and tribological behavior studies, were employed. It was revealed that laser alloying with carbide particulates affects structural, mechanical, and operational properties compared to cast iron in its initial state. Most importantly, the right choice of laser processing conditions can increase the wear resistance of the cast iron base. The wear resistance after WC alloying was 4-24 times higher compared to the initial material, while after SiC alloying, it was 2-18 times lower than that of the initial material.

Sublethal biochemical, behavioral, and physiological toxicity of extremely low dose of bendiocarb insecticide in Periplaneta americana (Blattodea: Blattidae).

Insecticides are dedicated to impair the insect organisms, but also have an impact on other, non-target organisms, including humans. In this way, they became important risk factor for disturbance of physiological homeostasis and can be involved in the development of diseases or in deterioration of existing conditions. The influence of sublethal doses of various insecticides on vertebrates' and invertebrates' organisms has been previously observed. In this paper, we have evaluated the impact of exposure to extremely low dose of neurotoxin, bendiocarb (0.1 nM), a commonly used carbamate insecticide on a model organism in neurobiology-Periplaneta americana. The assessment was performed on all levels of animal organism from molecular (oxidative stress parameters: phosphorylation level of proteins, cAMP level, protein kinase A and C levels, and octopamine) to physiological (heart beat and gas exchange tests) and behavioral (motor skills assay, grooming test). Exposure to such a low level of bendiocarb did not cause direct paralysis of insects, but changed their grooming behavior, decreased heart rate, and increased gas exchange. We also observed the increased parameters of oxidative stress as well as stressogenic response to 0.1 nM bendiocarb exposure. Exposure to a trace amount of bendiocarb also increased sensitivity to effective doses of the same insecticide, thus acts as preconditioning. These results force us to reconsider the possible risk from frequent/continuous exposure to traces of pesticide residues in the environment to human health.

Inertial Sensor-Based Sport Activity Advisory System Using Machine Learning Algorithms.

The aim of this study was to develop a physical activity advisory system supporting the correct implementation of sport exercises using inertial sensors and machine learning algorithms. Specifically, three mobile sensors (tags), six stationary anchors and a system-controlling server (gateway) were employed for 15 scenarios of the series of subsequent activities, namely squats, pull-ups and dips. The proposed solution consists of two modules: an activity recognition module (ARM) and a repetition-counting module (RCM). The former is responsible for extracting the series of subsequent activities (so-called scenario), and the latter determines the number of repetitions of a given activity in a single series. Data used in this study contained 488 three defined sport activity occurrences. Data processing was conducted to enhance performance, including an overlapping and non-overlapping window, raw and normalized data, a convolutional neural network (CNN) with an additional post-processing block (PPB) and repetition counting. The developed system achieved satisfactory accuracy: CNN + PPB: non-overlapping window and raw data, 0.88; non-overlapping window and normalized data, 0.78; overlapping window and raw data, 0.92; overlapping window and normalized data, 0.87. For repetition counting, the achieved accuracies were 0.93 and 0.97 within an error of ±1 and ±2 repetitions, respectively. The archived results indicate that the proposed system could be a helpful tool to support the correct implementation of sport exercises and could be successfully implemented in further work in the form of web application detecting the user's sport activity.

New View on the Impact of the Low-Frequency Electromagnetic Field (50 Hz) on Stress Responses: Hormesis Effect.

INTRODUCTION: Low-frequency electromagnetic field (50 Hz) (EMF) can modify crucial neuronal processes. Existing data indicate that exposure to EMF may represent a mild stressor and contribute to disturbances of the hypothalamic-pituitary-adrenal (HPA) axis. The important regulatory pathways controlling HPA axis activity include two types of corticosteroid receptors: mineralocorticoid receptors (MRs) and glucocorticoid receptors. They are particularly abundant in the hippocampus, a key locus of HPA axis feedback control. The research aimed at determining whether (1) EMF exhibits hormesis, it means bidirectional action depending on EMF intensity (1 or 7 mT) and (2) repeated EMF exposure changes stress response to subsequent stress factors. METHODS: The exposure (7 days, 1 h/day) of adult rats to EMF (1 mT and 7 mT) was repeated 3 times. HPA axis hormones and their receptors were analysed after each following exposure. Moreover, the impact of EMF exposure on hormonal and behavioural responses to subsequent stress factor - open-field test was evaluated. RESULTS: Our data suggest that exposure to EMF can establish a new "set-point" for HPA axis activity. The direction and dynamics of this process depend on the intensity of EMF and the number of exposures. EMF of 1 mT induced an adaptive stress response, but 7 mT EMF caused sensitization. Consequently, EMF changed the vulnerability of the organism to a subsequent stress factor. We have also shown the increase in MR mRNA abundance in the hippocampus of 1 mT EMF-exposed rats, which can represent the possible neuroprotective response and suggest therapeutic properties of EMFs.

Bidirectional Effect of Repeated Exposure to Extremely Low-Frequency Electromagnetic Field (50 Hz) of 1 and 7 mT on Oxidative/Antioxidative Status in Rat's Brain: The Prediction for the Vulnerability to Diseases.

Studies reported evidence for opposite effects of extremely low-frequency electromagnetic field (EMF): harmful, including the oxidative stress induction, and beneficial, such as the activation of antioxidant defense. People's exposure to EMF is often repeated or prolonged, and it is important to consider the cumulative effect of such kind of exposure on the organism. If changes evoked by repeated exposure to EMF are permanent, responsiveness to other stress factors can be modified. The aims of our study were (1) to evaluate changes in the levels of oxidative stress and antioxidant defense markers in the prefrontal cortex of adult rats after repeated exposure to 1 and 7 mT EMF and (2) to assess whether repeated EMF exposure can modify oxidative/antioxidative status in response to other stress factors. Rats were exposed to EMF 1 h/day for 7 days, one, twice, or three times. After each exposure, 8-isoprostanes, protein carbonyl groups, and the total antioxidant capacity were assessed. Part of the animals, after EMF treatment, was exposed to another stress factor-open field. Results showed that repeated exposure changed the oxidative/antioxidative status depending on the intensity of the EMF and the number of exposures. 1 mT EMF created weak changes in the oxidative status in the brain; however, 7 mT EMF moved the balance to a clearly higher level. The changes in the oxidative status after 1 mT EMF were enough to reduce, and after 7 mT EMF to intensify oxidative processes in response to the next stress. We concluded that the organism might adapt to "weak" EMF, while "strong" EMF exceeds the adaptive capacity of the organism and sensitizes it to subsequent stress, and thus may modulate vulnerability to diseases. Our results also provide new insights into the possible therapeutic properties of the magnetic field, as 1 mT EMF appears to have a potentially protective impact on the brain.

On the Features of Composite Coating, Based on Nickel Alloy and Aluminum-Iron Bronze, Processed by Direct Metal Deposition.

In recent years, additive manufacturing technologies have become increasingly widespread with the most intensive development being direct metal deposition (DMD), alloys, and ceramic materials on a metal substrate. This study shows the possibilities of the effective formation of coatings, based on heterogeneous metal alloys (Ni-based alloy and Fe-Al bronze) deposited onto 1045 structural steel. Changes in the microhardness, the microstructure, and the tribological properties of the composite coating, depending on the laser spot speed and pitch during DMD processing, have been considered. It was revealed that if the components of the composite coating are chosen correctly, there are possible DMD conditions ensuring reliable and durable connection between them and with the substrate.

On Investigating the Microstructural, Mechanical, and Tribological Properties of Hybrid FeGr1/SiC/Gr Metal Matrix Composites.

In recent years, studies of different properties of hybrid metal matrix composites, as well as very detailed issues, have been published. In this article, ready-made iron, graphite, and silicon carbide powders were used to produce the base material and composites. An analysis of some microstructural and mechanical properties, as well as the tribological behavior of metal matrix composites (MMCs), based on FeGr1 sintered material with the single and hybrid addition of a silicon carbide and graphite was undertaken. During the study, the flexural and compressive strength of MMCs were analyzed and changes of the momentary coefficient of friction, the temperature of friction, as well as wear rates of the MMCs tested were monitored. Based on the results, it was revealed that wear rates decreased 12-fold in comparison to the base material when SiC or SiC + Gr were added. Further research into MMCs with ceramic particle additives is proposed.

A Deep Learning-Based Model for the Automated Assessment of the Activity of a Single Worker.

Nowadays, it is necessary to verify the accuracy of servicing work, undertaken by new employees, within a manufacturing company. A gap in the research has been observed in effective methods to automatically evaluate the work of a newly employed worker. The main purpose of the study is to build a new, deep learning model, in order to automatically assess the activity of the single worker. The proposed approach integrates the methods known as CNN, CNN + SVM, CNN + R-CNN, four new algorithms and a piece of work from a selected company, using this as an own-created dataset, in order to create a solution enabling assessment of the activity of single workers. Data were collected from an operational manufacturing cell without any guided or scripted work. The results reveal that the model developed is able to accurately detect the correctness of the work process. The model's accuracy mostly exceeds current state-of-the-art methods for detecting work activities in manufacturing. The proposed two-stage approach, firstly, assigning the appropriate graphic instruction to a given employee's activity using CNN and then using R-CNN to isolate the object from the reference frames, yields 94.01% and 73.15% accuracy of identification, respectively.

Single Tracks as a Key Factor in Additive Manufacturing Technology-Analysis of Research Trends and Metal Deposition Behavior.

In recent years, general studies on Selective Laser Melting (SLM)/Selective Laser Sintering (SLS)/direct metal deposition (DMD) technologies, as well as studies on detailed issues in this area, have been carried out. However, a research gap is observed in investigations into the features of single tracks in the above-mentioned technologies. On the basis of data published in 2016-2019, an approach was adopted for a preliminary quantitative analysis of the knowledge base and also trends observed in the development of new technologies. This study demonstrates the effectiveness of the data mining technique based on the Bayes algorithm for analyzing trends in processes of additive manufacturing and the practical application of the knowledge received using the Bayes algorithm. After the analyses referred to above were completed, single and double layers of a composite material based on the Ni-based alloy and Fe-Al bronze were analyzed under different processing conditions. The effects of laser spot speeds and pitches on microhardness, microstructure, and interlayers' features were described. So, the innovative approach, namely, the combination of the analysis of the scientific database of the phenomenon under study and the subsequent experimental investigation of its features, is the scientific novelty of the present study.

Thermal conditions during neonatal anoxia affect the endogenous level of brain-derived neurotrophic factor.

Anoxia during delivery is a complication that can disturb infant brain development leading to various types of neurological disorders. Our studies have shown that increased body temperature of newborn rats of both sexes intensifies the postanoxic oxidative stress and prevents triggering the endogenous adaptive response such as HIF-1α activation. Currently, brain-derived neurotrophic factor-BDNF is considered to be a modulator of neuronal plasticity. In the developing brain, mature BDNF and its precursor exhibit prosurvival action through the TrkB receptor and proapoptotic functions binding to p75NTR , respectively. The aim of our experiments was to check the effects of body temperature on the postanoxic level of BDNF and on the expression of its receptors as well as on the marker of apoptosis-caspase-3 in the rat brain. Two-day-old Wistar Han rats (male/female ratio, 1:1) were exposed to anoxia in 100% nitrogen atmosphere for 10 min in different thermal conditions, which allowed them to regulate their rectal temperature at the following levels: normothermic-33°C; hyperthermic-37°C; and extremely hyperthermic-39°C. Thermal conditions during neonatal anoxia affected the level of proBDNF, BDNF as well as their receptors and caspase-3 in the forebrain. The increased BDNF protein level followed by decreased caspase-3 protein level was probably dependent on body temperature under anoxic conditions and was observed only in rats maintaining decreased body temperature. The positive effect of BDNF was not observed under hyperthermic conditions. Moreover, BDNF level changes correlated with body temperature probably affected the learning and spatial memory in juvenile rats.

Capsaicin-induced dysregulation of acid-base status in the American cockroach.

Members of TRP receptor family are involved in response to acidification. Here, we determined the effect of capsaicin, one of the TRP receptor activators, on hemolymph acid-base status in the American cockroach. Periplaneta americana adult individuals were injected with lactic acid (5% or 10%) and exposed to 100 µM capsaicin solution. Hemolymph pH was measured 15 min, 1, 4, 8 and 24 h after lactic acid and capsaicin application with a glass microelectrode. The results demonstrated that cockroaches recover from acidosis within 4 h from acid injection. Capsaicin impaired the buffering capacity of insects' hemolymph, resulting in significant drop of hemolymph pH observed even 24 h after application. Joint action of capsaicin and acidosis reveals new insight into possible mechanism of capsaicin action on TRP receptors in insects.

Effect of Capsaicin and Other Thermo-TRP Agonists on Thermoregulatory Processes in the American Cockroach.

Capsaicin is known to activate heat receptor TRPV1 and induce changes in thermoregulatory processes of mammals. However, the mechanism by which capsaicin induces thermoregulatory responses in invertebrates is unknown. Insect thermoreceptors belong to the TRP receptors family, and are known to be activated not only by temperature, but also by other stimuli. In the following study, we evaluated the effects of different ligands that have been shown to activate (allyl isothiocyanate) or inhibit (camphor) heat receptors, as well as, activate (camphor) or inhibit (menthol and thymol) cold receptors in insects. Moreover, we decided to determine the effect of agonist (capsaicin) and antagonist (capsazepine) of mammalian heat receptor on the American cockroach's thermoregulatory processes. We observed that capsaicin induced the decrease of the head temperature of immobilized cockroaches. Moreover, the examined ligands induced preference for colder environments, when insects were allowed to choose the ambient temperature. Camphor exposure resulted in a preference for warm environments, but the changes in body temperature were not observed. The results suggest that capsaicin acts on the heat receptor in cockroaches and that TRP receptors are involved in cockroaches' thermosensation.

Electromagnetic field exposure (50 Hz) impairs response to noxious heat in American cockroach.

Exposure to electromagnetic field (EMF) induces physiological changes in organism that are observed at different levels-from biochemical processes to behavior. In this study, we evaluated the effect of EMF exposure (50 Hz, 7 mT) on cockroach's response to noxious heat, measured as the latency to escape from high ambient temperature. We also measured the levels of lipid peroxidation and glutathione content as markers of oxidative balance in cockroaches exposed to EMF. Our results showed that exposure to EMF for 24, 72 h and 7 days significantly increases the latency to escape from noxious heat. Malondialdehyde (MDA) levels increased significantly after 24-h EMF exposure and remained elevated up to 7 days of exposure. Glutathione levels significantly declined in cockroaches exposed to EMF for 7 days. These results demonstrate that EMF exposure is a considerable stress factor that affects oxidative state and heat perception in American cockroach.

Altered heat nociception in cockroach Periplaneta americana L. exposed to capsaicin.

Some natural alkaloids, e.g. capsaicin and camphor, are known to induce a desensitization state, causing insensitivity to pain or noxious temperatures in mammals by acting on TRP receptors. Our research, for the first time, demonstrated that a phenomenon of pharmacological blockade of heat sensitivity may operate in American cockroach, Periplaneta americana (L.). We studied the escape reaction time from 50°C for American cockroaches exposed to multiple doses of different drugs affecting thermo-TRP. Capsaicin, capsazepine, and camphor induced significant changes in time spent at noxious ambient temperatures. Moreover, we showed that behavioral thermoregulation in normal temperature ranges (10-40°C) is altered in treated cockroaches, which displayed a preference for warmer regions compared to non-treated insects. We also measured the levels of malondialdehyde (MDA) and catalase activity to exclude the secondary effects of the drugs on these processes. Our results demonstrated that increase in time spent at 50°C (five versus one trial at a heat plate) induced oxidative stress, but only in control and vehicle-treated groups. In capsaicin, capsazepine, menthol, camphor and AITC-treated cockroaches the number of exposures to heat had no effect on the levels of MDA. Additionally, none of the tested compounds affected catalase activity. Our results demonstrate suppression of the heat sensitivity by repeated capsazepine, camphor and capsaicin administration in the American cockroach.

Pyrethroid residue dynamics in insects depends on the circadian clock.

Many factors may affect pesticide effectiveness against pests. One of the factors that should be considered is circadian rhythmicity. In this study, we evaluated daily variations in pyrethroid susceptibility in the house cricket, Acheta domesticus L. Crickets were exposed to a standard dose of ß-cyfluthrin at different times of a day, and pesticide residue levels were evaluated using gas chromatography. Results demonstrate that the time of pyrethroid disappearance is correlated with the circadian clock, with the highest decomposition rate at night. Furthermore, crickets also showed the highest resistance to the insecticide at night, expressed as a high survival rate. Moreover, ß-cyfluthrin induced significant changes in thermal preferences of intoxicated crickets. This is the first report showing that pyrethroid residue levels in the crickets' body depend on its circadian clock.