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2.
Nat Biomed Eng ; 4(1): 69-83, 2020 01.
Article in English | MEDLINE | ID: mdl-31844155

ABSTRACT

Exosomes are attractive as nucleic-acid carriers because of their favourable pharmacokinetic and immunological properties and their ability to penetrate physiological barriers that are impermeable to synthetic drug-delivery vehicles. However, inserting exogenous nucleic acids, especially large messenger RNAs, into cell-secreted exosomes leads to low yields. Here we report a cellular-nanoporation method for the production of large quantities of exosomes containing therapeutic mRNAs and targeting peptides. We transfected various source cells with plasmid DNAs and stimulated the cells with a focal and transient electrical stimulus that promotes the release of exosomes carrying transcribed mRNAs and targeting peptides. Compared with bulk electroporation and other exosome-production strategies, cellular nanoporation produced up to 50-fold more exosomes and a more than 103-fold increase in exosomal mRNA transcripts, even from cells with low basal levels of exosome secretion. In orthotopic phosphatase and tensin homologue (PTEN)-deficient glioma mouse models, mRNA-containing exosomes restored tumour-suppressor function, enhanced inhibition of tumour growth and increased survival. Cellular nanoporation may enable the use of exosomes as a universal nucleic-acid carrier for applications requiring transcriptional manipulation.


Subject(s)
Brain Neoplasms/drug therapy , Drug Delivery Systems , Electroporation/methods , Exosomes/metabolism , Glioma/drug therapy , RNA, Messenger/therapeutic use , Animals , Cells, Cultured , Disease Models, Animal , HEK293 Cells , Humans , Mice, Inbred C57BL , MicroRNAs/metabolism , MicroRNAs/therapeutic use , Nanotechnology , RNA, Messenger/metabolism , Signal Transduction
3.
Nat Nanotechnol ; 12(10): 974-979, 2017 10.
Article in English | MEDLINE | ID: mdl-28785092

ABSTRACT

Although cellular therapies represent a promising strategy for a number of conditions, current approaches face major translational hurdles, including limited cell sources and the need for cumbersome pre-processing steps (for example, isolation, induced pluripotency). In vivo cell reprogramming has the potential to enable more-effective cell-based therapies by using readily available cell sources (for example, fibroblasts) and circumventing the need for ex vivo pre-processing. Existing reprogramming methodologies, however, are fraught with caveats, including a heavy reliance on viral transfection. Moreover, capsid size constraints and/or the stochastic nature of status quo approaches (viral and non-viral) pose additional limitations, thus highlighting the need for safer and more deterministic in vivo reprogramming methods. Here, we report a novel yet simple-to-implement non-viral approach to topically reprogram tissues through a nanochannelled device validated with well-established and newly developed reprogramming models of induced neurons and endothelium, respectively. We demonstrate the simplicity and utility of this approach by rescuing necrotizing tissues and whole limbs using two murine models of injury-induced ischaemia.


Subject(s)
Cellular Reprogramming Techniques/methods , Fibroblasts/metabolism , Nanoparticles/chemistry , Transfection/methods , Animals , Cell Line , Endothelial Cells/metabolism , Endothelial Cells/pathology , Fibroblasts/pathology , Humans , Hypoxia/metabolism , Hypoxia/pathology , Hypoxia/therapy , Mice , Mice, Transgenic , Neurons/metabolism , Neurons/pathology
4.
Small ; 12(43): 5971-5980, 2016 Nov.
Article in English | MEDLINE | ID: mdl-27648733

ABSTRACT

While electroporation has been widely used as a physical method for gene transfection in vitro and in vivo, its application in gene therapy of cardiovascular cells remains challenging. Due to the high concentration of ion-transport proteins in the sarcolemma, conventional electroporation of primary cardiomyocytes tends to cause ion-channel activation and abnormal ion flux, resulting in low transfection efficiency and high mortality. In this work, a high-throughput nanoelectroporation technique based on a nanochannel array platform is reported, which enables massively parallel delivery of genetic cargo (microRNA, plasmids) into mouse primary cardiomyocytes in a controllable, highly efficient, and benign manner. A simple "dipping-trap" approach was implemented to precisely position a large number of cells on the nanoelectroporation platform. With dosage control, our device precisely titrates the level of miR-29, a potential therapeutic agent for cardiac fibrosis, and determines the minimum concentration of miR-29 causing side effects in mouse primary cardiomyocytes. Moreover, the dose-dependent effect of miR-29 on mitochondrial potential and homeostasis is monitored. Altogether, our nanochannel array platform provides efficient trapping and transfection of primary mouse cardiomyocyte, which can improve the quality control for future microRNA therapy in heart diseases.


Subject(s)
Myocytes, Cardiac/metabolism , Nanoparticles/chemistry , Nanotechnology/methods , Transfection/methods , Animals , Cells, Cultured , Computer Simulation , Electroporation , Mice , MicroRNAs/metabolism
5.
Nano Lett ; 16(9): 5326-32, 2016 09 14.
Article in English | MEDLINE | ID: mdl-27420544

ABSTRACT

Enhanced glioma-stem-cell (GSC) motility and therapy resistance are considered to play key roles in tumor cell dissemination and recurrence. As such, a better understanding of the mechanisms by which these cells disseminate and withstand therapy could lead to more efficacious treatments. Here, we introduce a novel micro-/nanotechnology-enabled chip platform for performing live-cell interrogation of patient-derived GSCs with single-clone resolution. On-chip analysis revealed marked intertumoral differences (>10-fold) in single-clone motility profiles between two populations of GSCs, which correlated well with results from tumor-xenograft experiments and gene-expression analyses. Further chip-based examination of the more-aggressive GSC population revealed pronounced interclonal variations in motility capabilities (up to ∼4-fold) as well as gene-expression profiles at the single-cell level. Chip-supported therapy resistance studies with a chemotherapeutic agent (i.e., temozolomide) and an oligo RNA (anti-miR363) revealed a subpopulation of CD44-high GSCs with strong antiapoptotic behavior as well as enhanced motility capabilities. The living-cell-interrogation chip platform described herein enables thorough and large-scale live monitoring of heterogeneous cancer-cell populations with single-cell resolution, which is not achievable by any other existing technology and thus has the potential to provide new insights into the cellular and molecular mechanisms modulating glioma-stem-cell dissemination and therapy resistance.


Subject(s)
Brain Neoplasms/pathology , Cell Movement , Glioblastoma/pathology , Neoplastic Stem Cells/cytology , Animals , Apoptosis , Humans , Mice , Tumor Cells, Cultured
6.
Nanoscale ; 8(1): 243-52, 2016 Jan 07.
Article in English | MEDLINE | ID: mdl-26309218

ABSTRACT

Of great interest to modern medicine and biomedical research is the ability to inject individual target cells with the desired genes or drug molecules. Some advances in cell electroporation allow for high throughput, high cell viability, or excellent dosage control, yet no platform is available for the combination of all three. In an effort to solve this problem, here we show a "3D nano-channel electroporation (NEP) chip" on a silicon platform designed to meet these three criteria. This NEP chip can simultaneously deliver the desired molecules into 40,000 cells per cm(2) on the top surface of the device. Each 650 nm pore aligns to a cell and can be used to deliver extremely small biological elements to very large plasmids (>10 kbp). When compared to conventional bulk electroporation (BEP), the NEP chip shows a 20 fold improvement in dosage control and uniformity, while still maintaining high cell viability (>90%) even in cells such as cardiac cells which are characteristically difficult to transfect. This high-throughput 3D NEP system provides an innovative and medically valuable platform with uniform and reliable cellular transfection, allowing for a steady supply of healthy, engineered cells.


Subject(s)
Electroporation/instrumentation , Electroporation/methods , Transfection/instrumentation , Transfection/methods , Animals , Cell Line , Cell Survival , Humans , Mice
7.
Nanomedicine ; 12(2): 399-409, 2016 Feb.
Article in English | MEDLINE | ID: mdl-26711960

ABSTRACT

Safety concerns and/or the stochastic nature of current transduction approaches have hampered nuclear reprogramming's clinical translation. We report a novel non-viral nanotechnology-based platform permitting deterministic large-scale transfection with single-cell resolution. The superior capabilities of our technology are demonstrated by modification of the well-established direct neuronal reprogramming paradigm using overexpression of the transcription factors Brn2, Ascl1, and Myt1l (BAM). Reprogramming efficiencies were comparable to viral methodologies (up to ~9-12%) without the constraints of capsid size and with the ability to control plasmid dosage, in addition to showing superior performance relative to existing non-viral methods. Furthermore, increased neuronal complexity could be tailored by varying BAM ratio and by including additional proneural genes to the BAM cocktail. Furthermore, high-throughput NEP allowed easy interrogation of the reprogramming process. We discovered that BAM-mediated reprogramming is regulated by AsclI dosage, the S-phase cyclin CCNA2, and that some induced neurons passed through a nestin-positive cell stage. FROM THE CLINICAL EDITOR: In the field of regenerative medicine, the ability to direct cell fate by nuclear reprogramming is an important facet in terms of clinical application. In this article, the authors described their novel technique of cell reprogramming through overexpression of the transcription factors Brn2, Ascl1, and Myt1l (BAM) by in situ electroporation through nanochannels. This new technique could provide a platform for further future designs.


Subject(s)
Basic Helix-Loop-Helix Transcription Factors/genetics , Cellular Reprogramming , DNA-Binding Proteins/genetics , DNA/administration & dosage , Nerve Tissue Proteins/genetics , Neurons/cytology , POU Domain Factors/genetics , Transcription Factors/genetics , Transfection/methods , Animals , Cell Line , DNA/genetics , Electroporation/methods , Fibroblasts/cytology , Fibroblasts/metabolism , Humans , Mice , Neurons/metabolism , Plasmids/administration & dosage , Plasmids/genetics , Up-Regulation
8.
Lab Chip ; 15(15): 3147-53, 2015 Aug 07.
Article in English | MEDLINE | ID: mdl-26105628

ABSTRACT

Current transfection technologies lead to significant inter-clonal variations. Previously we introduced a unique electrotransfection technology, Nanochannel-Electroporation (NEP), which can precisely and benignly transfect small cell populations (~100-200 cells) with single-cell resolution. Here we report on the development of a novel 3D NEP system for large scale transfection. A properly-engineered array of nanochannels, capable of handling/transfecting ~60 000 cells cm(-2), was fabricated using cleanroom technologies. Positive dielectrophoresis was used to selectively position cells on the nanochannels, thus allowing highly efficient transfection. Single-cell dosage control was demonstrated using both small and large molecules, and different cell types. The potential clinical relevance of this system was tested with difficult-to-transfect natural killer cell suspensions, and plasmids encoding for the chimeric antigen receptor (CAR), a model of high relevance for adoptive immunotherapy. Our results show significantly higher CAR transfection efficiencies for the DEP-NEP system (>70% vs. <30%), as well as enhanced cell viabilities.


Subject(s)
Electroporation/methods , Immunotherapy, Adoptive/methods , Nanotechnology/methods , Transfection/methods , Animals , Cell Line , Electroporation/instrumentation , Equipment Design , Humans , Immunotherapy, Adoptive/instrumentation , Killer Cells, Natural/cytology , Nanotechnology/instrumentation , Rats , Transfection/instrumentation , Transgenes
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