ABSTRACT
Luminol-dependent chemiluminescence (CL) was used to assay the effect of three antituberculous drugs [isoniazid (INH), pyrazinamide (PZA), rifampicin (RM)] on the production of reactive oxygen species (ROS) by polymorphonuclear leukocytes (PMN) isolated from blood of 5 healthy donors. Drugs were added directly to the medium in concentrations; INH 5 micrograms/ml, PZA 40 micrograms/ml, RM 7 micrograms/ml. These concentrations correspond to peak serum concentrations after usual doses. RM was found to inhibit FMLP (N-formyl-methionyl-leucyl -phenylalanine)-induced CL response of PMN, PZA did not influence the metabolic activity of neutrophils, and INH stimulated PMN CL.
Subject(s)
Antitubercular Agents/pharmacology , Isoniazid/pharmacology , Neutrophils/drug effects , Pyrazinamide/pharmacology , Reactive Oxygen Species/metabolism , Rifampin/pharmacology , Humans , Luminescent Measurements , Neutrophils/metabolismABSTRACT
Tolpa Peat Preparation (TPP) administered in various doses does not influence the ability of mononuclear cells (MNC) obtained from diabetic patients with proliferative retinopathy to induce neovascularization response in H-LIA test. Methylxanthines (theophylline, theobromine) significantly decrease the angiogenic activity of these cells. The therapeutic value of these drugs in proliferative retinopathy should be evaluated.
Subject(s)
Amino Acids/pharmacology , Carbohydrates/pharmacology , Diabetic Retinopathy/pathology , Humic Substances/pharmacology , Leukocytes, Mononuclear/drug effects , Neovascularization, Pathologic/etiology , Theobromine/pharmacology , Theophylline/pharmacology , Uronic Acids/pharmacology , Adolescent , Adult , Animals , Child , Drug Combinations , Humans , Leukocytes, Mononuclear/physiology , Mice , Mice, Inbred BALB C , SoilABSTRACT
The influence of vit. E treatment on metabolic and bactericidal granulocyte activity in patients with recurrent infections was studied by chemiluminescence and phagocytosis assays. Vit. E serum level was significantly diminished before therapy. Six weeks supplementation with 200-300 mg/day of vit. E resulted in improvement of clinical status and normalisation of alpha-tocopherol serum level. Bactericidal activity of granulocytes was lower in patients than in the respective controls and it slightly rose after vit. E therapy. Before, therapy, patients granulocytes showed metabolic activity above or within the control range. Vit. E treatment increased, had no effect or decreased granulocyte metabolic activity, but never below the control range.
Subject(s)
Bacterial Infections/drug therapy , Granulocytes/drug effects , Vitamin E/pharmacology , Adult , Aged , Bacterial Infections/etiology , Bacterial Infections/physiopathology , Blood Bactericidal Activity/drug effects , Female , Free Radicals , Granulocytes/physiology , Humans , Luminescent Measurements , Male , Middle Aged , Oxygen/metabolism , Recurrence , Vitamin E/blood , Vitamin E Deficiency/complications , Vitamin E Deficiency/physiopathologyABSTRACT
T-cells subpopulations were determined with theophylline test in 42 patients with the ischemic heart disease, including 31 patients with the history of myocardial infarction, and in 78 healthy volunteers. The patients' age was between 20 and 40 years and between 60 and 80 years. Defect of theophylline-sensitive fraction (suppressor T-cells and cytotoxically not specific lymphocytes in young patients has been observed. This defect gradually diminished with patients' age. Moreover, and increase in the percentage of theophylline-resistant fraction of the delayed rosettes, containing among other helper T-cells for the production of antibodies, and a decrease in the percentage of early rosette fraction containing precursor cells have been noted in the group of young patients. This defect is normalized in the group of older patients.
Subject(s)
Coronary Disease/blood , T-Lymphocytes/immunology , Adult , Aged , Aged, 80 and over , Aging/blood , Humans , Middle Aged , Rosette Formation , TheophyllineABSTRACT
Using the lymphocyte-induced angiogenesis test a comparison of angiogenic activity of some mononuclear cell fractions was performed. It is shown that the highest angiogenic activity was expressed by cells bearing both the receptor for the Fe portion of IgG and a CD4 surface antigen.
Subject(s)
Immunity, Cellular , Leukocytes, Mononuclear/immunology , Neovascularization, Pathologic , Adult , Animals , Antigens, Differentiation/immunology , Antigens, Differentiation, T-Lymphocyte/immunology , Cell Separation , Humans , Leukocytes, Mononuclear/classification , Leukocytes, Mononuclear/transplantation , Mice , Mice, Inbred BALB C , Receptors, Fc/immunology , Receptors, IgGABSTRACT
The clinical course of 38 patients with Hodgkin's disease in the stage of remission was evaluated. The material consisted of two groups of patients: 1. submitted to immunotherapy and 2. control group. In 16 cases immunostimulation was performed with BCG vaccine, including four cases treated with BCG vaccine and levamisole and 3 patients were given levamisole only. In some patients, immunotherapy restored normal immunologic reactivity, as shown by the reversion of negative tests of delayed hypersensitivity. In all patients treated with levamisole caused normal blastic transformation of PHA-stimulated lymphocytes. In the group of patients treated with immunotherapy relapses of malignant lymphogranulomatosis were observed in 10.6% of cases, and in the control group--in 15.7%. The patients of both groups remain under continuous clinical surveillance.
Subject(s)
BCG Vaccine/therapeutic use , Hodgkin Disease/therapy , Levamisole/therapeutic use , Adolescent , Adult , Cell Migration Inhibition , Female , Hodgkin Disease/immunology , Humans , Immunotherapy , Leukocyte Count , Lymphocyte Activation , Lymphocytes/immunology , Male , Middle Aged , Rosette FormationABSTRACT
In the lymphocytes of 20 healthy subjects and 17 patients with chronic lymphatic leukaemia (CLL) the cAMP level was determined before and at different time periods during cell incubation with mitogens (phytohaemagglutinin--PHA, concanavalin A--Con A, and pokeweed mitogen PWM). The experiments demonstrated that PHA-induced lymphocyte proliferation was associated in healthy subjects during the first 10--20 minutes of incubation with a rise in the intracellular cAMP concentration, while the level of cAMP decreased systematically during 30 minutes of incubation with Con A and PWM. Incubation of lymphocytes of the patients with CLL during 30 minutes with PHA, Con A and PWM caused no changes in the low cAMP level in these cells. The obtained results suggest that PHA activation of lymphocytes in healthy subjects is connected with changes in the intracellular concentration of cAMP.
Subject(s)
Body Fluids/metabolism , Cyclic AMP/metabolism , Intracellular Fluid/metabolism , Leukemia, Lymphoid/blood , Lymphocytes/metabolism , Mitogens/pharmacology , Adult , Aged , Cells, Cultured , Concanavalin A/pharmacology , Female , Humans , Male , Middle Aged , Phytohemagglutinins/pharmacology , Pokeweed Mitogens/pharmacology , Stimulation, ChemicalABSTRACT
In 40 cases of Hodgkin's disease (24 untreated patients and 16 patients in remission) the immunological humoral and cell-mediated competence was studied using test of hypersensitivity of DNCB and PPD, blastic transformation and the ability of 3H-DNA synthesis in lymphocytes stimulated with PHA and PWM, and in some cases, with PPD. In all patients the concentration of serum immunoglobulins was determined as well. Impairment of immunological reactivity was found in about 40% of patients, on the average, in various stages of the disease and during remissions following treatment with cytostatic agents and radiotherapy. The most frequent abnormality found in 52.5% of cases was impairment of blastic transformation of lymphocytes stimulated with PHA in vitro. In 60% of cases negative results of intradermal test with PPD and in 75% of patients the DNCB test was negative. No correlation could be found between impairment of the immune competence in this disease and the clinical stage and/or type of histological changes in lymph nodes.
Subject(s)
Hodgkin Disease/immunology , T-Lymphocytes/immunology , Cells, Cultured , Child , Child, Preschool , DNA/biosynthesis , Dinitrochlorobenzene/pharmacology , Female , Humans , Immune Tolerance , In Vitro Techniques , Lymphocyte Activation , Male , Phytohemagglutinins/pharmacology , Pokeweed Mitogens/pharmacology , Stimulation, ChemicalABSTRACT
Deoxyribonucleic acid (DNA) synthesis was investigated in vitro in T and B lymphocyte populations isolated from peripheral blood. The synthesis was determined by measurements of 3H-thymidine incorporation. Only T lymphocytes isolated from peripheral blood of healthy subjects as well as patients with chronic lymphocytic leukemia (cll) showed proliferative ability after stimulation with phytohemagglutinin (PHA). DNA synthesis rate in normal T lymphocytes was enhanced by exogenous cyclic adenosine monophosphate (cAMP) added to lymphocyte culture together with PHA in concentrations of 10(-5) and 10(-4)M. Higher concentrations (10(-3) and 10(-2)M) of this nucleotide inhibited DNA synthesis in the lymphocytes of healthy subjects as well as cell patients. Impairment of 3H-thymidine incorporation observed in cll (in non-isolated cell populations) seems to be due to the presence of non-proliferating lymphocyte B population.
