Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 2 de 2
Filter
Add more filters










Database
Type of study
Language
Publication year range
1.
J Food Prot ; 70(11): 2617-9, 2007 Nov.
Article in English | MEDLINE | ID: mdl-18044444

ABSTRACT

Resistance of Escherichia coli O157 to inactivation by high-pressure processing, heat, and UV and gamma radiation was associated with the allele of the prophage-encoded antiterminator Q gene present upstream of the Shiga toxin gene stx2. Increased processing may be required to kill certain strains of E. coli O157, and the choice of strains used as surrogate markers for processing efficiency is critical.


Subject(s)
Bacterial Proteins/genetics , Escherichia coli O157/growth & development , Food Handling/methods , Food Microbiology , Prophages , RNA-Binding Proteins/genetics , Alleles , Colony Count, Microbial , Escherichia coli O157/drug effects , Escherichia coli O157/radiation effects , Gamma Rays , Hot Temperature , Pressure , Ultraviolet Rays
2.
Appl Environ Microbiol ; 72(4): 2661-71, 2006 Apr.
Article in English | MEDLINE | ID: mdl-16597971

ABSTRACT

Seventeen Escherichia coli O157:H7 strains were treated with ultrahigh pressure at 500 MPa and 23 +/- 2 degrees C for 1 min. This treatment inactivated 0.6 to 3.4 log CFU/ml, depending on the strain. The diversity of these strains was confirmed by pulsed-field gel electrophoresis (PFGE) analysis, and there was no apparent association between PFGE banding patterns and pressure resistance. The pressure-resistant strain E. coli O157:H7 EC-88 (0.6-log decrease) and the pressure-sensitive strain ATCC 35150 (3.4-log decrease) were treated with a sublethal pressure (100 MPa for 15 min at 23 +/- 2 degrees C) and subjected to DNA microarray analysis using an E. coli K-12 antisense gene chip. High pressure affected the transcription of many genes involved in a variety of intracellular mechanisms of EC-88, including the stress response, the thiol-disulfide redox system, Fe-S cluster assembly, and spontaneous mutation. Twenty-four E. coli isogenic pairs with mutations in the genes regulated by the pressure treatment were treated with lethal pressures at 400 MPa and 23 +/- 2 degrees C for 5 min. The barotolerance of the mutants relative to that of the wild-type strains helped to explain the results obtained by DNA microarray analysis. This study is the first report to demonstrate that the expression of Fe-S cluster assembly proteins and the fumarate nitrate reductase regulator decreases the resistance to pressure, while sigma factor (RpoE), lipoprotein (NlpI), thioredoxin (TrxA), thioredoxin reductase (TrxB), a trehalose synthesis protein (OtsA), and a DNA-binding protein (Dps) promote barotolerance.


Subject(s)
Bacterial Proteins/metabolism , Escherichia coli O157/physiology , Gene Expression Profiling , Heat-Shock Response , Hydrostatic Pressure , Bacterial Proteins/genetics , Electrophoresis, Gel, Pulsed-Field , Escherichia coli O157/genetics , Escherichia coli O157/metabolism , Food Preservation/methods , Gene Expression Regulation, Bacterial , Humans , Oligonucleotide Array Sequence Analysis
SELECTION OF CITATIONS
SEARCH DETAIL
...