Toxigenic Escherichia coli with high antibiotic resistance index recovered from sands of recreational beaches of Mumbai, India.

Mumbai, India's seven-island city, is known for its sandy beaches as a major tourist attraction, but urbanization and industrialization have weakened the environment. Unregulated sewage disposal and untreated effluents off the coast have made the beach environment vulnerable. Therefore, monitoring water and sand quality at beaches should be mandatory. This study was thus designed to determine the microbiological status of selected sandy beaches, viz. Versova, Juhu, and Girgaon. The study found fecal coliforms in the sand, with stx1 and stx2 genes specific for Shiga toxin-producing E. coli pathotypes in 5.5 % of isolates, whereas the presence of eaeA gene specific for enteropathogenic E. coli pathotype was detected in 12.2 % of isolates, and the presence of the LT and ST genes specific for enterotoxigenic E. coli pathotype was detected in 6.6 % of isolates. Multiple antibiotic-resistant indices indicated high-risk contamination sources. The study suggests routine monitoring of pollution levels at coastal cities' beaches.

Ecobiology and distribution of Streptomyces species from distinctive metal polluted sediment of tropical estuary, Northwest coast of India.

The present study aims to isolate diverse actinomycetes communities from the estuarine sediments of Patalganga located at Northwest coast of India. Total 40 actinomycetes were isolated from 24 sediment samples by dilution plating on six different isolation media. Among them, morphologically 18 distinct selected isolates of actinomycetes were verified by 16S rRNA gene sequencing and identified as Streptomyces spp. The relationship between diversity of total actinomycetes population (TAP) and its antagonistic behaviour with the physico-chemical characteristics of sediment samples were investigated. Multiple regression analysis revealed that the influencing physico-chemical factors comprises of sediment temperature, sediment pH, organic carbon and heavy metals. The results of statistical analysis showed that TAP is positively correlated (p < 0.01) with sediment organic carbon whereas, negatively correlated with Cr (p < 0.05) and Mn (p < 0.01). Based on Principle component Analysis (PCA) and cluster analysis results, the obtained six stations can be divided into three groups. Among them mobile fraction of metals, TAP could be the main factor reflecting the lower and middle estuary. The recovery of large number of actinomycete isolates suggests that Patalganga Estuary could be potential source of bioactive compounds with biosynthetic capabilities.

Pathogenic multiple antimicrobial resistant Escherichia coli serotypes in recreational waters of Mumbai, India: a potential public health risk.

Globally, coastal waters have emerged into a pool of antibiotic resistance genes and multiple antibiotic resistant microorganisms, and pathogenicity of these resistant microorganisms in terms of serotypes and virulence genes has made the environment vulnerable. The current study underscores the presence of multiple antibiotic resistant pathogenic serotypes and pathotypes of Escherichia coli, the predominant faecal indicator bacteria (FIB), in surface water and sediment samples of famous recreational beaches (Juhu, Versova, Mahim, Dadar, and Girgaon) of Mumbai. Out of 65 faecal coliforms (FC) randomly selected, 38 isolates were biochemically characterized, serotyped (for 'O' antigen), antibiogram-phenotyped (for 22 antimicrobial agents), and genotyped by polymerase chain reaction (for virulence factors). These isolates belonged to 16 different serotypes (UT, O141, O2, O119, O120, O9, O35, O126, O91, O128, O87, O86, R, O101, O118, and O15) out of which UT (18.4%), O141 (15.7%), and O2 (13.1%) were predominant, indicating its remarkable diversity. Furthermore, the generated antibiogram profile revealed that 95% of these isolates were multiple antibiotic resistant. More than 60% of aminoglycoside-sensitive E. coli isolates exhibited resistance to penicillin, extended penicillin, quinolone, and cephalosporin classes of antibiotic while resistance to other antibiotics was comparatively less. Antibiotic resistance (AR) indexing indicated that these isolates may have rooted from a high-risk source of contamination. Preliminary findings revealed the presence of enterotoxin-encoding genes (stx1 and stx2 specific for enterohaemorrhagic E. coli and Shiga toxin-producing E. coli, heat-stable toxin enterotoxin specific for enterotoxigenic E. coli) in pathogenic serotypes. Thus, government authorities and environmental planners should create public awareness and adopt effective measures for coastal management to prevent serious health risks associated with these contaminated coastal waters.