ABSTRACT
Catalytic signal amplification was used for the colorimetric detection of CN(-) in aqueous media by using the enzyme catalase in tandem with mesoporous silica nanoparticle based synthetic HRP enzyme mimic Fe-MSNs. Signal amplification up to a maximum of eight fold was observed for the reporter "oxidized TMB" with respect to the added CN(-) ion.
Subject(s)
Amides/chemistry , Biuret/chemistry , Catalase/chemistry , Cyanides/analysis , Ferric Compounds/chemistry , Nanoparticles/chemistry , Silicon Dioxide/chemistry , Catalase/metabolism , Catalysis , Colorimetry , Molecular Structure , Porosity , Surface PropertiesABSTRACT
Trypsin has been encapsulated in the mesopores of a hierarchical mesoporous silica material synthesized via Cu(I) catalyzed azide-alkyne click reaction between azide functionalized large spherical SBA-15 particles and alkyne functionalized mesoporous silica nanoparticles (MSNs). Encapsulated trypsin functions as an efficient biocatalyst and can be recycled several times.
Subject(s)
Trypsin/chemistry , Alkynes/chemistry , Azides/chemistry , Catalysis , Click Chemistry , Copper/chemistry , Nanoparticles/chemistry , Porosity , Silicon Dioxide/chemistry , Trypsin/metabolismABSTRACT
An [Fe(III)(biuret-amide)] complex has been immobilized onto mesoporous silica nanoparticles via Cu(I) catalyzed azide-alkyne click chemistry. This hybrid material functions as an efficient peroxidase mimic and was successfully used for the quantitative determination of hydrogen peroxide and glucose via a one-pot colorimetric assay.
