ABSTRACT
Improper handling of jet fuel at abandoned military bases has resulted in heavy pollution of the soil and groundwater. Experimental research of photocatalytical oxidation (PCO) of jet fuel aqueous extract and aqueous solutions of de-icing agents was undertaken. The influence of different parameters - pH, concentration of substances to be oxidised, presence of inorganic admixtures, effect of OH. radical generators--on the PCO of solutions of de-icing agents and jet fuel aqueous extract was determined. The role of OH. radicals was found to be less important in determining the PCO rate. The PCO of organic pollutants was also investigated using a catalyst immobilised onto the surface of buoyant hollow glass microspheres. Attached titanium dioxide (TiO2) showed lower photocatalytical activity than when suspended in slurry, although it allows waters to be treated in simple shallow ponds without intensive stirring. The biodegradability of aqueous solutions of de-icing agents and jet fuel aqueous extract increased as PCO proceeded.
Subject(s)
Ethylene Glycol/chemistry , Hydrocarbons/chemistry , Soil Pollutants/analysis , Water Pollutants/analysis , Catalysis , Coloring Agents/chemistry , Oxidation-Reduction , Photochemistry , Titanium/chemistryABSTRACT
The interaction of deoxyribonucleoside-5'-mono-, di- and triphosphates with E. coli DNA polymerase I Klenow fragments was examined. Dissociation constants of the enzyme complex with nucleotides were determined from the data on the enzyme inactivation by adenosine 2',3'-riboepoxide 5'-triphosphate. The role of nucleotide bases, phosphate groups and sugar moieties in the complex formation of nucleotides with the enzyme was elucidated. The necessity of complementary interaction of nucleotides with templates for template-controlled 'adjusting' of complementary dNTP to its reactive state was found. The crucial role of the interaction of dNTP gamma-phosphate with the enzyme in this process is discussed.
Subject(s)
DNA-Directed DNA Polymerase/metabolism , Deoxyribonucleotides/metabolism , Binding Sites , Escherichia coli/enzymology , Kinetics , Substrate Specificity , Templates, Genetic , ThermodynamicsABSTRACT
A case of fungiform mycosis with involvement of the liver, kidneys, and the skin in a female patient aged 56 is described. Duration of the illness--2 months. Lesions of the skin localized on the face and upper extremities. The anamnesis revealed chronic bronchitis and bronchial asthma of a 15-year duration. The corticosteroid therapy resulted in destruction of tumour-like nodes on the skin, but the patient died because of a concomitant bilateral fibrous-purulent pneumonia. The diagnosis was established on clinical data (on bioptic specimens of the skin) and corroborated by sectional material.
